1887

Abstract

Virus-like particles were purified from banana plants with banana bunchy top disease. These particles were isometric with a diameter of 18 to 20 nm and a density of 1.28 to 1.30 g/ml in caesium sulphate. Associated with these particles were an ssDNA of about 1 kb and one major protein of 20100. DsDNA was synthesized from nucleic acid extracts from these particles and cloned. One clone, pBT338, hybridized specifically (i) with sap extracts from plants infected with banana bunchy top virus (BBTV) but not with sap extracts from healthy plants and (ii) with the small ssDNA in nucleic acid extracts from infected plants and virus-like particles. Banana bunchy top disease was transmitted from infected to healthy bananas by aphid inoculation and it was demonstrated that the small ssDNA was transmitted with the disease. It is probable that these particles represent the virions of BBTV containing small ssDNA and that the virus resembles subterranean clover stunt virus more than any other known virus.

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/content/journal/jgv/10.1099/0022-1317-72-2-225
1991-02-01
2024-04-24
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References

  1. Blum H., Beier H., Gross H. J. 1987; Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 8:93–99
    [Google Scholar]
  2. Chu P. W. G., Helms K. 1988; Novel virus-like particles containing circular single-stranded DNA associated with subterranean clover stunt disease. Virology 167:38–49
    [Google Scholar]
  3. Dale J. L. 1987; Banana bunchy top: an economically important tropical plant virus disease. Advances in Virus Research 33:301–325
    [Google Scholar]
  4. Dale J. L., Phillips D. A., Parry J. N. 1986; Double-stranded RNA in banana plants with bunchy top disease. Journal of General Virology 67:371–375
    [Google Scholar]
  5. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  6. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. Journal of Molecular Biology 166:557–580
    [Google Scholar]
  7. Iskra M. L., Garnier M., Bove J. M. 1989; Purification of banana bunchy top virus. Fruits 44:63–66
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. McMaster G. K., Carmichael G. G. 1977; Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A 74:4835–4838
    [Google Scholar]
  10. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–199
    [Google Scholar]
  12. Murant A. F., Waterhouse P. M., Raschké J. H., Robinson D. J. 1985; Carrot red leaf and carrot mottle viruses: observations on the composition of the particles in single and mixed infections. Journal of General Virology 66:1575–1579
    [Google Scholar]
  13. Su H. J., Wu R. Y. 1989; Characterisation and monoclonal antibodies of the virus causing banana bunchy top. ASPAC Food and Fertiliser Technology Center, Taipei, Technical Bulletin no. 115, May, 1989 10 pp
    [Google Scholar]
  14. Thomas J. E., Dietzgen R. G. 1991; Purification, characterization and serological detection of virus-like particles associated with banana bunchy top disease in Australia. Journal of General Virology 72:217–224
    [Google Scholar]
  15. Upcroft P., Healey A. 1987; Rapid and efficient method for cloning of blunt-ended DNA fragments. Gene 51:69–75
    [Google Scholar]
  16. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  17. Wu R. Y., Su H. J. 1990; Purification and characterization of banana bunchy top virus. Journal of Phytopathology 128:153–160
    [Google Scholar]
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