1887

Abstract

The bovine herpesvirus type 1 (BHV-1) protein VP8 is present, in large amounts, in the tegument of virions. As a preliminary step towards determining the function of VP8 and the biological relevance for its abundant presence, we describe the mapping of the location of its gene and determination of its nucleotide sequence. The gene for VP8 was located between 0.088 and 0.108 map units on the BHV-1 genome and contained a 2226 bp reading frame encoding a 742 amino acid protein. The protein, produced by transcribing and translating the reading frame, was precipitated by monoclonal antibodies and polyclonal serum directed against VP8. The primary structure of VP8 showed considerable homology with the product of the UL47 reading frame of herpes simplex virus type 1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-12-3077
1991-12-01
2022-06-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/12/JV0720123077.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-12-3077&mimeType=html&fmt=ahah

References

  1. Bandyopadhyay S. K., Mittal S. K., Field H. J. 1990; Identification of the gene homologous to HSV-1 major DNA binding protein in the BHV-l genome. Veterinary Microbiology 22:203–212
    [Google Scholar]
  2. Barker D. E., Roizman B. 1990; Identification of three genes nonessential for growth in cell culture-near the right terminus of the unique sequences of long component of herpes simplex virus 1. Virology 177:684–691
    [Google Scholar]
  3. Berk A. J., Sharp P. A. 1977; Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell 12:721–732
    [Google Scholar]
  4. Bolton D. C., Zee Y. C., Ardans A. A. 1983; Identification of envelope and nucleocapsid proteins of infectious bovine rhinotra-cheitis virus by SDS-polyacrylamide gel electrophoresis. Veterinary Microbiology 8:57–68
    [Google Scholar]
  5. Cathala G., Savouret J. F., Mendez B., West B. L., Karin M., Martial J. A., Baxter J. D. 1983; Laboratory methods: a method for isolation of intact, translationally active ribonucleic acid. DNA 2:329–335
    [Google Scholar]
  6. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  7. Dayhoff M. (editor) 1978 Atlas of Protein Sequence and Structure vol 5 supplement 3, pp 345–358 Washington, D. C.: National Biomedical Research Foundation;
    [Google Scholar]
  8. Fitzpatrick D., Babiuk L. A., Zamb T. J. 1989; Nucleotide sequence of bovine herpesvirus type 1 glycoprotein gIII, a structural model as a new member of the immunoglobulin superfamily and implications for the homologous glycoproteins of other herpes viruses. Virology 173:46–57
    [Google Scholar]
  9. Goding C. R., O’Hare P. 1989; Herpes simplex virus Vmw65-octamer binding protein interaction: a paradigm for combinatorial control of transcription. Virology 173:363–367
    [Google Scholar]
  10. Hall L. M., Draper K. G., Frink R. J., Costa R. H., Wagner E. K. 1982; Herpes simplex virus mRNA species maping in EcoRI fragment I. Journal of Virology 43:594–607
    [Google Scholar]
  11. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignments on a micro computer. Gene 73:237–244
    [Google Scholar]
  12. Lemaster S., Roizman B. 1980; Herpes simplex virus phosphopro-teins. II. Characterization of the virion protein kinase and of the polypeptides phosphorylated in the virion. Journal of Virology 35:798–811
    [Google Scholar]
  13. Ludwig G. V., Letchworth G. J. III 1987; Temporal control of bovine herpesvirus 1 glycoprotein synthesis. Journal of Virology 61:3292–3294
    [Google Scholar]
  14. McGeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., McNab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type I. Journal of General Virology 69:1531–1574
    [Google Scholar]
  15. McKnight J. L. C., Pellett P. E., Jenkins F. J., Roizman B. 1987; Characterization and nucleotide sequence of two herpes simplex virus 1 genes whose products modulate α-trans-inducing factor-dependent activation of the α genes. Journal of Virology 61:992–1001
    [Google Scholar]
  16. McLean G., Rixon F., Langeland N., Haarr L., Marsden H. 1990; Identification and characterization of the virion protein products of herpes simplex virus type 1 gene UL47. Journal of General Virology 71:2953–2960
    [Google Scholar]
  17. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Marshall R. L., Rodriguez L. L., & Letchworth G. J. III 1986; Characterization of envelope proteins of infectious bovine rhinotra-cheitis virus (bovine herpesvirus) by biochemical and immunological methods. Journal of Virology 57:745–753
    [Google Scholar]
  19. Misra V., Blumenthal R. M., Babiuk L. A. 1981; Proteins specified by bovine herpesvirus-1 (infectious bovine rhinotracheitis virus). Journal of Virology 40:367–378
    [Google Scholar]
  20. Misra V., Gilchrist J. E., Weinmaster G., Qualtiere L., van den Hurk S., Babiuk L. A. 1982; A herpesvirus induced ‘early’ glycoprotein: characterization and possible role in immune cytolysis. Journal of Virology 43:1046–1054
    [Google Scholar]
  21. Misra V., Nelson R., Smith M. 1988; Sequence of a bovine heresvirus type-1 glycoprotein gene that is homologous to the herpes simplex gene for the glycoprotein gB. Virology 166:542–549
    [Google Scholar]
  22. Mittal S. K., Field J. 1989; Analysis of the bovine herpesvirus type 1 thymidine kinase (TK) gene from wild-type and TK-deficient mutants. Journal of General Virology 70:901–918
    [Google Scholar]
  23. Nelson R., Adachi A.-M., Chisholm H., Misra V. 1989; Temperature-sensitive mutants of bovine herpesvirus type 1: mutants which make unaltered levels of ‘early’ glycoproteins but fail to synthesize a ‘late’ glycoprotein. Journal of General Virology 70:125–132
    [Google Scholar]
  24. Owen L. J., Field H. J. 1988; Genomic location and sequence analysis of the putative bovine herpesvirus-1 polymerase gene. Archives of Virology 98:27–38
    [Google Scholar]
  25. Pastoret P. P., Burtonboy G., Aguilar-Setien A., Godart M., Lamy M. E., Schoenaers F. 1980; Comparison between strains of infectious bovine rhinotracheitis virus (bovid herpesvirus I) from respiratory and genital origins, using polyacrylamide gel electrophoresis of structural proteins. Veterinary Microbiology 5:187–192
    [Google Scholar]
  26. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA-dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:493–497
    [Google Scholar]
  27. Roizman B., Kristie T., McKnight J. L. C., Michael N., Mavromara-Nazos P., Spector D. 1988; The trans-activation of herpes simplex virus gene expression: comparison of two factors and their cis sites. Biochimie 70:1031–1043
    [Google Scholar]
  28. Smith G. A., Young P. L., Mattick J. S. 1990; The location and nucleotide sequence of the thymidine kinase gene of bovine herpesvirus type 1.2. Journal of General Virology 71:2417–2424
    [Google Scholar]
  29. Stern S., Tanaka M., Herr W. 1989; The oct-1 homoeodomain directs formation of a multiprotein-DNA complex with HSV transactivator VP16. Nature, London 341:624–630
    [Google Scholar]
  30. Tikoo S. K., Fitzpatrick D. R., Babiuk L. A., Zamb T. J. 1990; Molecular cloning, sequencing and expression of functional bovine herpesvirus I glycoprotein gIV in transfected bovine cells. Journal of Virology 64:5132–5142
    [Google Scholar]
  31. Wirth U. V., Gunkel K., Engels M., Schwyzer M. 1989; Spatial and temporal distribution of bovine herpesvirus 1 transcripts. Journal of Virology 63:4882–4889
    [Google Scholar]
  32. Whitbeck J. C., Bello L. J., Lawrence W. C. 1988; Comparison of the bovine herpesvirus IgI gene and the herpes simplex virus type 1 gB gene. Journal of Virology 62:3319–3327
    [Google Scholar]
  33. Zhang Y., Sirko D. A., McKnight J. L. 1991; Role of herpes simplex virus type 1 UL46 and UL47 in alpha TIF-mediated transcriptional induction: characterization of three viral deletion mutants. Journal of Virology 65:829–841
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-12-3077
Loading
/content/journal/jgv/10.1099/0022-1317-72-12-3077
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error