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Volume 72, Issue 12

The DNA-binding Domain of Nuclear Factor I is Sufficient to Cooperate with the Adenovirus Type 2 DNA-binding Protein in Viral DNA Replication Free

Abstract

Recombinant baculoviruses have been constructed which express the full-length nuclear factor I (NFI) protein or a derivative of NFI that contains only the DNA-binding domain of the protein in infected insect cells. Both proteins were purified from insect cells infected with the respective baculoviruses and tested for their ability to cooperate with the adenovirus type 2 (Ad2) DNA-binding protein during virus replication. DNase I protection experiments demonstrated that the viral DNA-binding protein increased the affinity of both the full-length NFI and the DNA-binding domain of NFI for their recognition site in the Ad2 origin of DNA replication. As a consequence, the NFI-dependent increase in the efficiency of DNA replication observed upon addition of viral DNA-binding protein was the same when the full-length or DNA-binding domain derivative of NFI was added. Thus it appears that all of the activities associated with the ability of NFI to stimulate Ad2 DNA replication are located within the DNA-binding domain of the protein.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1991
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1991-12-01
2024-04-25
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References

  1. Bosher J., Robinson E. C., Hay R. T. 1990; Interactions between the adenovirus type 2 DNA polymerase and the DNA binding domain of nuclear factor I. New Biologist 2:1083–1090
     [Google Scholar]
  2. Challberg M. D., Rawlins D. R. 1984; Template requirements for the initiation of adenovirus DNA replication. Proceedings of the National Academy of Sciences, U.S.A. 81:100–104
     [Google Scholar]
  3. Chen M., Mermod N., Horwitz M. S. 1990; Protein-protein interactions between adenovirus DNA polymerase and nuclear factor I mediate formation of the DNA replication preinitiation complex. Journal of Biological Chemistry 265:18634–18642
     [Google Scholar]
  4. Clark L., Matthews J. R., Hay R. T. 1990; Interaction of enhancer-binding protein EBP1 (NF-kB) with the human immunodeficiency virus type 1 enhancer. Journal of Virology 64:1335–1344
     [Google Scholar]
  5. Cleat P. H., Hay R. T. 1989a; Co-operative interactions between NFI and the adenovirus DNA binding protein at the adenovirus origin of DNA replication. EMBO Journal 8:1841–1848
     [Google Scholar]
  6. Cleat P. H., Hay R. T. 1989b; A kinetic analysis of nuclear factor I and its DNA binding domain with the adenovirus origin of DNA replication. FEBS Letters 258:51–54
     [Google Scholar]
  7. De Vries E., van Driel W., Tromp M., van Boom I., van der Vliet P. C. 1985; Adenovirus DNA replication in vitro: site-directed mutagenesis of the nuclear factor I binding site of the Ad2 origin. Nucleic Acids Research 13:4935–4952
     [Google Scholar]
  8. Goding C. R., Russell W. C. 1983; Adenovirus cores can function as templates in in vitro DNA replication. EMBO Journal 2:339–344
     [Google Scholar]
  9. Gounari F., De Francesco R., Schmitt J., van der Vliet P. C., Cortese R., Stunnenberg H. 1990; Amino-terminal domain of NF-I binds to DNA as a dimer and activates adenovirus DNA replication. EMBO Journal 9:559–566
     [Google Scholar]
  10. Guggenheimer R. A., Stillman B. W., Nagata K., Tamanoi F., Hurwitz J. 1984; DNA sequences required for the in vitro replication of adenovirus DNA. Proceedings of the National Academy of Sciences, U.S.A. 81:3069–3073
     [Google Scholar]
  11. Harris M. P. G., Hay R. T. 1988; DNA sequences required for the initiation of adenovirus type 4 DNA replication in vitro. Journal of Molecular Biology 201:57–67
     [Google Scholar]
  12. Hay R. T. 1985a; The origin of adenovirus DNA replication: minimal DNA sequence requirement in vivo. EMBO Journal 4:421–426
     [Google Scholar]
  13. Hay R. T. 1985b; The origin of adenovirus DNA replication: role of the nuclear factor I binding site in vivo. Journal of Molecular Biology 186:129–136
     [Google Scholar]
  14. Hay R. T., McDougall I. M. 1986; Viable viruses with deletions in the left inverted terminal repeat define the adenovirus origin of DNA replication. Journal of General Virology 67:321–332
     [Google Scholar]
  15. Hay R. T., Russell W. C. 1989; Recognition mechanisms in the synthesis of animal virus DNAs. Journal of Biochemistry 258:3–16
     [Google Scholar]
  16. Hay R. T., Stow N. D., McDougall I. M. 1984; Replication of adenovirus minichromosomes. Journal of Molecular Biology 175:493–510
     [Google Scholar]
  17. Jones K. A., Kadonaga J. T., Rosenfeld P. J., Kelly T. J., Than R. 1987; A cellular DNA-binding protein that activates eukaryotic transcription and DNA replication. Cell 48:79–89
     [Google Scholar]
  18. Kenny M. K., Hurwitz J. 1988; Initiation of adenovirus DNA replication. Journal of Biological Chemistry 263:9809–9817
     [Google Scholar]
  19. Lally C., Dörper T., Gröger W., Antoine G., Winnacker E. -L. 1984; A size analysis of the adenovirus replicon. EMBO Journal 3:333–337
     [Google Scholar]
  20. Leith I. R., Hay R. T., Russell W. C. 1989; Adenovirus subviral particles and cores can support limited DNA replication. Journal of General Virology 70:3235–3248
     [Google Scholar]
  21. Lindenbaum J. O., Field J., Hurwitz J. 1986; The adenovirus DNA binding protein and adenovirus DNA polymerase interact to catalyze elongation of primed DNA templates. Journal of Biological Chemistry 261:10218–10227
     [Google Scholar]
  22. Meisterernst M., Rogge L., Foeckler R., Karaghiosoff M., Winnacker E. -L. 1989; Structural and functional organisation of a porcine gene coding for nuclear factor I. Biochemistry 28:8191–8200
     [Google Scholar]
  23. Mermod N., O’Neill E. A., Kelly T. J., Than R. 1989; The proline-rich transcriptional activator of CTF/NF-I is distinct from the replication and DNA binding domain. Cell 58:741–753
     [Google Scholar]
  24. Mul Y. M., Verrijzer C. P., van der Vliet P. C. 1990; Transcription factors NFI and NFIII/oct-1 function independently, employing different mechanisms to enhance adenovirus DNA replication. Journal of Virology 64:5510–5518
     [Google Scholar]
  25. Nagata K., Guggenheimer R. A., Enomoto T., Lichy J. H., Hurwitz J. 1982; Adenovirus DNA replication in vitro: identification of a host factor that stimulates synthesis of the preterminal protein-dCMP complex. Proceedings of the National Academy of Sciences, U.S.A. 79:6438–6442
     [Google Scholar]
  26. Nagata K., Guggenheimer R. A., Hurwitz J. 1983; Specific binding of a cellular DNA replication protein to the origin of replication of adenovirus DNA. Proceedings of the National Academy of Sciences, U.S.A. 80:6177–6181
     [Google Scholar]
  27. Paonessa G., Gounari F., Frank R., Cortese R. 1988; Purification of a NFI-like DNA-binding protein from rat liver and cloning of the corresponding cDNA. EMBO Journal 7:3115–3123
     [Google Scholar]
  28. Possee R. D., Howard S. C. 1987; Analysis of the polyhedrin gene promoter of the Autographa catifomica nuclear polyhedrosis virus. Nucleic Acids Research 15:10233–10248
     [Google Scholar]
  29. Rosenfeld P. J., O’Neill E. A., Wides R. J., Kelly T. J. 1987; Sequence-specific interactions between cellular DNA-binding proteins and the adenovirus origin of replication. Molecular Cell Biology 7:875–886
     [Google Scholar]
  30. Santoro C., Mermod N., Andrews P. C., Tjian R. 1988; A family of human CCAAT-box-binding proteins active in transcription and DNA replication: cloning and expression of multiple cDNAs. Nature, London 334:218–224
     [Google Scholar]
  31. Stuiver M. H., van der Vliet P. C. 1990; Adenovirus DNA binding protein forms a multimeric protein complex with doublestranded DNA and enhances binding of nuclear factor I. Journal of Virology 65:379–389
     [Google Scholar]
  32. Temperley S. M., Hay R. T. 1991; Replication of adenovirus type 4 DNA by a purified fraction from infected cells. Nucleic Acids Research 19:3243–3249
     [Google Scholar]
  33. Watson C. J., Hay R. T. 1990; Expression of adenovirus type 2 DNA polymerase in insect cells infected with a recombinant baculovirus. Nucleic Acids Research 18:1167–1173
     [Google Scholar]
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The DNA-binding Domain of Nuclear Factor I is Sufficient to Cooperate with the Adenovirus Type 2 DNA-binding Protein in Viral DNA Replication
J. Gen. Virol. 72, 2975 (1991); https://doi.org/10.1099/0022-1317-72-12-2975
/content/journal/jgv/10.1099/0022-1317-72-12-2975
/content/journal/jgv/10.1099/0022-1317-72-12-2975
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