The Nucleotide Sequence and Deduced Amino Acid Composition of the Haemagglutinin and Fusion Proteins of the Morbillivirus Phocid Distemper Virus Free

Abstract

The amino acid composition of the two surface proteins of the recently isolated morbillivirus phocid distemper virus (PDV) were deduced from the nucleotide sequence. The fusion (F) protein of PDV exhibited characteristics similar to those of other morbillivirus F proteins. The overall amino acid similarity with its closest homologue, canine distemper virus (CDV), was 72%. From the context of the starting codons and the requirement for a hydrophobic signal peptide, it is likely that translation of the PDV F mRNA starts at the third AUG, corresponding to codon 95 in the long open reading frame of the PDV F gene. After removal of the signal peptide, F starts at amino acid 105. From this position the F protein of PDV and CDV exhibit 84% amino acid similarity. The PDV haemagglutinin (H) protein showed 74% amino acid similarity with CDV H protein and highly conserved features responsible for the tertiary structure. Despite these similarities, the two H proteins show marked antigenic differences when probed with monoclonal antibodies. Earlier studies have indicated that rinderpest virus (RPV) is the prototype virus of the morbillivirus genus, from which first CDV/PDV and later measles virus (MV) evolved. From the close relationship shown in this study, it is likely that the divergence of CDV and PDV occurred after MV evolved from RPV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-12-2959
1991-12-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/12/JV0720122959.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-12-2959&mimeType=html&fmt=ahah

References

  1. Alkhatib G., Briedis D. J. 1986; The predicted primary structure of the measles virus hemagglutinin. Virology 150:479–490
    [Google Scholar]
  2. Appel M. J. G. 1987; Canine distemper virus. In Virus Infections of Vertebrates vol 1 Edited by Horzinek M. C. Amsterdam: Elsevier;
    [Google Scholar]
  3. Appel M. J. G., Shek W. R., Sheshberadaran H., Norrby E. 1984; Measles virus and inactivated canine distemper virus induce incomplete immunity to canine distemper virus. Archives of Virology 82:73–82
    [Google Scholar]
  4. Barrett T., Clarke D. K., Evans S. A., Rima B. K. 1987; The nucleotide sequence of the gene encoding the F protein of canine distemper virus: a comparison of the deduced amino acid sequence with other paramyxoviruses. Virus Research 8:373–386
    [Google Scholar]
  5. Blixenkrone-Möller M., Svansson V., Have P., Bötner A., Nielsen J. 1989; Infection studies in mink with seal-derived morbillivirus. Archives of Virology 106:165–170
    [Google Scholar]
  6. Cosby S. L., McQuaid S., Duffy N., Lyons C., Rima B. K., Allan G. M., McCullough S. J., Kennedy S., McNeilly F., Craig C., Örvell C. 1988; Characterization of a seal morbillivirus. Nature, London 336:115–116
    [Google Scholar]
  7. Curran M. D., O’Loan D., Rima B. K., Kennedy S. 1990; Nucleotide sequence analysis of phocid distemper virus reveals its distinctness from canine distemper virus. Veterinary Record 127:430–431
    [Google Scholar]
  8. Curran M. D., Clarke D. K., Rima B. K. 1991; The nucleotide sequence of the gene encoding the attachment protein H of canine distemper virus. Journal of General Virology 72:443–447
    [Google Scholar]
  9. Evans S. A., Belsham G. J., Barrett T. 1990; The role of the 5′ nontranslated regions of the fusion protein mRNAs of canine distemper and rinderpest virus. Virology 177:317–323
    [Google Scholar]
  10. Giraudon P., Wild T. F. 1985; Correlation between epitopes on hemagglutinin of measles virus and biological activities. Passive protection by monoclonal antibodies is related to their hemagglutinating activity. Virology 144:46–58
    [Google Scholar]
  11. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  12. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. III. Structural differences of Sendai virus grown in eggs and tissue culture cells. Journal of Virology 12:1457–1465
    [Google Scholar]
  13. Itoh M., Shibuta H., Homma M. 1987; Single amino acid substitution of Sendai virus at the cleavage site of the fusion protein confers trypsin resistance. Journal of General Virology 68:2939–2944
    [Google Scholar]
  14. Katz R. A., Cullen B. R., Malavarca R., Skalka A. M. 1986; Role of the avian retrovirus mRNA leader in expression: evidence for novel translation control. Molecular and Cellular Biology 6:372–379
    [Google Scholar]
  15. Kingsbury D. W. 1990; Paramyxoviridae and their replication. In Virology, 2nd edn.. pp 945–962 Edited by Fields B. N., Knipe. D. M. New York: Raven Press;
    [Google Scholar]
  16. Kingsbury D. W., Bratt M. A., Choppin P. W. 1978; Paramyxoviridae. Intervirology 10:137–152
    [Google Scholar]
  17. Kövamees J., Rydbeck R., Örvell C., Norrby E. 1990; Hemagglutinin-neuraminidase (HN) amino acid alterations in neutralization escape mutants of Kilham mumps virus. Virus Research 17:119–130
    [Google Scholar]
  18. Kövamees J., Blixenkrone-Möller M., Norrby E. 1991; The nucleotide and predicted amino acid sequence of the attachment protein of canine distemper virus. Virus Research 19:223–234
    [Google Scholar]
  19. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  20. Mahy B. W. J., Barrett T., Evans S., Anderson E. C., Bostock C. J. 1988; Characterization of a seal morbillivirus. Nature, London 336:115
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning. A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Marck C. 1988; DNA strider: a “C” program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers. Nucleic Acids Research 16:1829–1836
    [Google Scholar]
  23. Norrby E. 1989; Modern approaches to live virus vaccines. Advances in Veterinery Science and Comparative Medicine 33:249–270
    [Google Scholar]
  24. Norrby E., Oxman M. N. 1990; Measles. In Virology, 2nd. edn., pp 1013–1044 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  25. Norrby E., Sheshberadaran H., McCullough K. C., Carpenter W. C., Örvell C. 1985; Is rinderpest virus the archevirus of the morbillivirus genus?. Intervirology 23:228–232
    [Google Scholar]
  26. Örvell C. 1980; Structural polypeptides of canine distemper virus. Archives of Virology 66:193–206
    [Google Scholar]
  27. Örvell C., Blixenkrone-Möller M., Svansson V., Have P. 1990; Immunological relationships between phocid and canine distemper virus studied with monoclonal antibodies. Journal of General Virology 71:2085–2092
    [Google Scholar]
  28. Osterhaus A. D. M. E. 1989; A morbillivirus causing mass mortality in seals. Vaccine 7:483–484
    [Google Scholar]
  29. Osterhaus A. D. M. E., Groen J., De Vreis P., UytdeHaag F. G. M. C., Klingeborn B., Zarnke R. 1988; Canine distemper virus in seals. Nature, London 335:403–404
    [Google Scholar]
  30. Richardson C. D., Scheid A., Choppin P. W. 1980; Specific inhibition of paramyxovirus and myxovirus replication by oligopeptides with amino acid sequences similar to those at the N-termini of the F1 or HA2 viral polypeptides. Virology 105:205–222
    [Google Scholar]
  31. Richardson C. D., Hull D., Greer P., Hasel K., Berkovich A., Englund G., Bellini W., Rima B., Lazzarini R. 1986; The nucleotide sequence of the mRNA encoding the fusion protein of measles virus (Edmonston strain): a comparison of fusion proteins from several different paramyxoviruses. Virology 155:508–523
    [Google Scholar]
  32. Sambrook J., Fritsch E. F., Maniatis T. 1989; Molecular Cloning. A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. Sanger F., Nicklen S., & Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A. 74:5463–5467
    [Google Scholar]
  34. Sato T. A., Hayami M., Yamanouchi K. 1981; Analysis of structural proteins of measles, canine distemper and rinderpest viruses. Japanese Journal of Medical Science and Biology 34:355–364
    [Google Scholar]
  35. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, hemolysis and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  36. Sheshberadaran H., Norrby E., McCullough K. C., Carpenter W. C., Örvell C. 1986; The antigenic relationship between measles, canine distemper and rinderpest viruses studied with monoclonal antibodies. Journal of General Virology 67:1381–1392
    [Google Scholar]
  37. Shimizu K., Ishida N. 1975; The smallest protein of Sendai virus: its candidate function of binding nucleocapsid to envelope. Virology 67:427–437
    [Google Scholar]
  38. Tsukiyama K., Sugiyama M., Yoshikawa Y., Yamanouchi K. 1987; Molecular cloning and sequence analysis of the rinderpest virus mRNA encoding the hemagglutinin protein. Virology 160:48–54
    [Google Scholar]
  39. Tsukiyama K., Yoshikawa Y., Yamanouchi K. 1988; Fusion glycoprotein (F) of rinderpest virus: entire nucleotide sequence of the F mRNA, and several features of the F protein. Virology 164:523–530
    [Google Scholar]
  40. Varsanyi T. M., Kovamees J., Norrby E. 1991; Molecular cloning and sequence analysis of human parainfluenza type 2 virus mRNA encoding the fusion glycoprotein. Journal of General Virology 11:89–95
    [Google Scholar]
  41. Visser I. K. G., Van Der Bildt M. W. G., Brugge H. N., Reijnders P. J. H., Vedder E. J., Kuiper J., de Vreis P., Groen J., Walvoort H. C., UytdeHaag F. G. C. M., Osterhaus A. D. M. E. 1989; Vaccination of harbour seals (Phoca vitulina) against phocid distemper with two different inactivated canine distemper virus (CDV) vaccines. Vaccine 7:521–526
    [Google Scholar]
  42. Woods D. 1984; Oligonucleotide screening of cDNA libraries. Focus 6:3
    [Google Scholar]
  43. Zhang H., School R., Browsee J., Sommerville C. 1988; Double-stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Research 16:1220
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-12-2959
Loading
/content/journal/jgv/10.1099/0022-1317-72-12-2959
Loading

Data & Media loading...

Most cited Most Cited RSS feed