@article{mbs:/content/journal/jgv/10.1099/0022-1317-72-11-2853, author = "Osman, T. A. M. and Buck, K. W.", title = "Detection of the movement protein of red clover necrotic mosaic virus in a cell wall fraction from infected Nicotiana clevelandii plants", journal= "Journal of General Virology", year = "1991", volume = "72", number = "11", pages = "2853-2856", doi = "https://doi.org/10.1099/0022-1317-72-11-2853", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-11-2853", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The movement protein of red clover necrotic mosaic virus (RCNMV) was expressed in Escherichia coli as a fusion with a maltose-binding protein using the vector pMAL-cRI and used to produce an antiserum. The RCNMV movement protein was detected in a cell wall fraction obtained from infected Nicotiana clevelandii leaf tissue by immunoblotting using the movement protein antiserum. The movement protein could be detected 6 h after inoculation and reached a maximum after 24 h. In contrast, the virus capsid protein, detected in a soluble fraction by immunoblotting using a capsid antiserum, continued to increase for 72 h after inoculation.", }