1887

Abstract

The movement protein of red clover necrotic mosaic virus (RCNMV) was expressed in as a fusion with a maltose-binding protein using the vector pMAL-cRI and used to produce an antiserum. The RCNMV movement protein was detected in a cell wall fraction obtained from infected leaf tissue by immunoblotting using the movement protein antiserum. The movement protein could be detected 6 h after inoculation and reached a maximum after 24 h. In contrast, the virus capsid protein, detected in a soluble fraction by immunoblotting using a capsid antiserum, continued to increase for 72 h after inoculation.

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1991-11-01
2024-04-16
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References

  1. Albrecht H., Geldreich A., Menissier de Murcia J., Kirchherr D., Mesnard J. -M., Lebeurier G. 1988; Cauliflower mosaic virus gene 1 product detected in a cell wall-enriched fraction. Virology 163:503–508
    [Google Scholar]
  2. Allison R. F., Janda M., Ahlquist P. 1989; Sequence of cowpea chlorotic mottle virus RNAs 2 and 3 and evidence of a recombination event during bromovirus evolution. Virology 172:321–330
    [Google Scholar]
  3. Argos P. 1988; A sequence motif in many polymerases. Nucleic Acids Research 16:9909–916
    [Google Scholar]
  4. Atabekov J. G., Taliansky M. E. 1990; Expression of a plant virus-coded transport function by different viral genomes. Advances in Virus Research 38:201–248
    [Google Scholar]
  5. Atkins D., Hull R., Wells B., Roberts K., Moore P., Beachy R. N. 1991; The tobacco mosaic virus 30K movement protein in transgenic tobacco plants is localized to plasmodesmata. Journal of General Virology 72:209–211
    [Google Scholar]
  6. Buck K. W. 1990; Virus resistant plants. In Plant Genetic Engineering vol 1 pp 136–178 Edited by Grierson D. London & Glasgow: Blackie & Son;
    [Google Scholar]
  7. Citovsky V., Knorr D., Zambryski P. 1991; Gene I, a potential cell-to-cell movement locus of cauliflower mosaic virus, encodes an RNA-binding protein. Proceedings of the National Academy of Sciences, U.S.A. 88:2476–2480
    [Google Scholar]
  8. Godefroy-Colburn T., Gagey M. -J., Berna A., Stussi-Garaud C. 1986; A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. Journal of General Virology 67:2233–2239
    [Google Scholar]
  9. Gould A. R., Francki R. I. B., Hatta T., Hollings M. 1981; The bipartite genome of red clover necrotic mosaic virus. Virology 108:499–506
    [Google Scholar]
  10. Habili N., Symons R. H. 1989; Evolutionary relationship between luteoviruses and other RNA plant viruses based on sequence motifs in their putative RNA polymerases and nucleic acid helicases. Nucleic Acids Research 17:9543–9555
    [Google Scholar]
  11. Hull R. 1989; The movement of viruses in plants. Annual Review of Phytopathology 27:213–240
    [Google Scholar]
  12. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods in Enzymology 154:367–382
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Lehto K., Bubrick P., Dawson W. O. 1990; Time course of TMV 30K protein accumulation in intact leaves. Virology 174:290–293
    [Google Scholar]
  15. Linstead P. J., Hills G. J., Plaskitt K. A., Wilson I. G., Harker C. L., Maule A. J. 1988; The subcellular location of the gene 1 product of cauliflower mosaic virus is consistent with a function associated with virus spread. Journal of General Virology 69:1809–1818
    [Google Scholar]
  16. Lommel S. A., Weston-Fina M., Lomonossoff G. P. 1988; The nucleotide sequence and gene organization of red clover necrotic mosaic virus RNA-2. Nucleic Acids Research 16:8587–8601
    [Google Scholar]
  17. Maina C. V., Riggs P. D., Grandea A. G., Slatko B. E., Moran L. S., Tagliamonte J. A., McReynolds L. A., Guan C. 1988; A vector to express and purify foreign proteins in Escherichia coli by fusion to, and separation from, maltose binding protein. Gene 74:365–373
    [Google Scholar]
  18. Marriott A. C., Buck K. W. 1988; Genome organization and expression of red clover necrotic mosaic virus. In Viruses with Fungal Vectors pp 111–122 Edited by Cooper J. I., Asher M. J. C. Wellesbourne: Association of Applied Biologists;
    [Google Scholar]
  19. Morris-Krsinich B. A. M., Forster R. L. S., Mossop D. W. 1983; Translation of red clover necrotic mosaic virus RNA in rabbit reticulocyte lysate: identification of the virus coat protein cistron on the larger RNA strand of the bipartite genome. Virology 124:349–356
    [Google Scholar]
  20. Moser O., Gagey M. -J., Godefroy-Colburn T., Stussi-Garaud C., Ellwart-Tschürtz M., Nitschko H., Mundry K. -W. 1988; The fate of the transport protein of tobacco mosaic virus in systemic and hypersensitive tobacco hosts. Journal of General Virology 69:1367–1373
    [Google Scholar]
  21. Okuno T., Hiruki C., Rao D. V., Figueiredo G. C. 1983; Genetic determinants distributed in two genomic RNAs of sweet clover necrotic mosaic, red clover necrotic mosaic and clover primary leaf necrosis viruses. Journal of General Virology 64:1907–1914
    [Google Scholar]
  22. Osman T. A. M., Buck K. W. 1987; Replication of red clover necrotic mosaic virus RNA in cowpea protoplasts: RNA 1 replicates independently of RNA 2. Journal of General Virology 68:289–296
    [Google Scholar]
  23. Osman T. A. M., Buck K. W. 1990; Double-stranded RNAs isolated from plant tissue infected with red clover necrotic mosaic virus correspond to genomic and subgenomic single-stranded RNAs. Journal of General Virology 71:945–948
    [Google Scholar]
  24. Osman T. A. M., Dodd S. M., Buck K. W. 1986; RNA 2 of red clover necrotic mosaic virus determines lesion morphology and systemic invasion in cowpea. Journal of General Virology 67:203–207
    [Google Scholar]
  25. Osman T. A. M., Miller S. J., Marriott A. C., Buck K. W. 1991a; Nucleotide sequence of RNA 2 of a Czechoslovakian isolate of red clover necrotic mosaic virus. Journal of General Virology 72:213–216
    [Google Scholar]
  26. Osman T. A. M., Ingles P. J., Miller S. J., Buck K. W. 1991b; A spontaneous red clover necrotic mosaic virus mutant with a truncated movement protein. Journal of General Virology 72:1793–1800
    [Google Scholar]
  27. Paje-Manalo L., Lommel S. A. 1989; Independent replication of red clover necrotic mosaic virus RNA-1 in electroporated host and non-host Nicotiana species protoplasts. Phytopathology 79:457–461
    [Google Scholar]
  28. Robards A. W., Lucas W. J. 1990; Plasmodesmata. Annual Review of Plant Physiology 41:369–419
    [Google Scholar]
  29. Sherwood J. L. 1987; Comparison of a filter paper immunobinding assay, Western blotting and an enzyme-linked immunosorbent assay for the detection of wheat streak mosaic virus. Journal of Phytopathology 118:68–75
    [Google Scholar]
  30. Stussi-Garaud C., Garaud J. -C., Berna A., Godefroy-Colburn T. 1987; In situ location of alfalfa mosaic virus non-structural protein in plant cell walls: correlation with virus transport. Journal of General Virology 68:1779–1784
    [Google Scholar]
  31. Tomenius K., Clapham D., Meshi T. 1987; Localization by immunogold cytochemistry of the virus coded 30K protein in plasmodesmata of leaves infected with tobacco mosaic virus. Virology 160:363–371
    [Google Scholar]
  32. Xiong Z., Lommel S. A. 1989; The complete nucleotide sequence and genome organization of red clover necrotic mosaic virus RNA-1. Virology 171:543–554
    [Google Scholar]
  33. Xiong Z., Lommel S. A. 1991; Red clover necrotic mosaic virus infectious transcripts synthesized in vitro. Virology 182:388–392
    [Google Scholar]
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