1887

Abstract

The nucleotide sequences of DNAs complementary to the eighth (S8) and the tenth (S10) largest of the 12 genome segments of rice gall dwarf virus (RGDV) were determined. The S8 and S10 segments consist of 1578 and 1198 nucleotides, each with a single open reading frame extending for 1278 nucleotides from nucleotide 21, and 960 nucleotides from nucleotide 22, respectively. S8 encodes a polypeptide of 426 amino acids with an of 47419. The amino acid sequences of several peptide fragments of the major outer capsid protein reported as 45K were contained in the predicted polypeptide. This protein, renamed the 47K protein, showed high homology with the outer capsid proteins of rice dwarf virus (RDV) and wound tumour virus (WTV); there was 56, 52 and 48% amino acid sequence identity between RGDV and WTV, RGDV and RDV, and RDV and WTV, respectively. S10 had the coding potential for a polypeptide of 320 amino acids with an of 36095 (36K protein), which exhibits 32% and 35% amino acid sequence identity with the predicted translation product of RDV S9 and the P9 capsid protein encoded by WTV S11, respectively. The conserved terminal sequences 5′ GG…GAU 3′ which are present in all genome segments of WTV and RDV so far analysed, and in S9 of RGDV, were also found in RGDV S8 and S10. This conserved sequence together with the segment-specific inverted repeats found in the terminal sequence of RGDV S8 and S10 are thus characteristic structures common to all three phytoreoviruses. The nucleotide sequence of the region surrounding the inverted repeats was more similar between RGDV and WTV than between RGDV and RDV.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-11-2837
1991-11-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/11/JV0720112837.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-11-2837&mimeType=html&fmt=ahah

References

  1. Anzola J. V., Xu Z., Asamizu T., Nuss D. L. 1987; Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Proceedings of the National Academy of Sciences, U.S.A. 84:8301–8305
    [Google Scholar]
  2. Anzola J. V., Dall D. J., Xu Z., Nuss D. L. 1989; Complete nucleotide sequence of wound tumor virus genomic segments encoding non-structural polypeptides. Virology 171:222–228
    [Google Scholar]
  3. Dall D. J., Anzola J. V., Xu Z., Nuss D. L. 1989; Complete nucleotide sequence of wound tumor virus genomic segment S11. Nucleic Acids Research 17:3599
    [Google Scholar]
  4. Dayhoff M. O., Eck R. V., Park C. M. 1972; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure vol 5 pp 89–99 Edited by Dayhoff M. O. Washington, D.C.: National Biomedical Research Foundation;
    [Google Scholar]
  5. Fukumoto F., Omura T., Minobe Y. 1989; Nucleotide sequence of segment S9 of the rice dwarf virus genome. Archives of Virology 107:135–139
    [Google Scholar]
  6. Kano H., Koizumi M., Noda H., Mizuno H., Tsukihara T., Ishikawa K., Hibino H., Omura T. 1990; Nucleotide sequence of rice dwarf virus (RD V) genome segment S3 coding for 114K major core protein. Nucleic Acids Research 18:6700
    [Google Scholar]
  7. Kimura I., Shikata E. 1968; Structural model of rice dwarf virus. Proceedings of the Japan Academy 44:538–543
    [Google Scholar]
  8. Koganezawa H., Hibino H., Motoyoshi F., Kato H., Noda H., Ishikawa K., Omura T. 1990; Nucleotide sequence of segment S9 of the genome of rice gall dwarf virus. Journal of General Virology 71:1861–1863
    [Google Scholar]
  9. Liu H. Y., Black L. M. 1978; Neutralization of infectivity of potato yellow dwarf virus and wound tumor virus assayed on vectorcell monolayers. Phytopathology 68:1243–1248
    [Google Scholar]
  10. Matsuoka M., Minobe Y., Omura T. 1986; Reaction of antiserum against SDS-dissociated rice dwarf virus and a polypeptide of rice gall dwarf virus. Phytopathology 75:1125–1127
    [Google Scholar]
  11. Mizuno H., Omura T., Koizumi M., Kano H., Kondoh M., Tsukihara T. 1990; Crystallographic studies of double shelled spherical viruses, rice dwarf virus. Proceedings of the XVth International Congress and General Assembly International Union of Crystallography, Bordeaux, France, C-89
    [Google Scholar]
  12. Nakashima K., Kakutani T., Minobe Y. 1990; Sequence analysis and product assignment of segment 7 of the rice dwarf virus genome. Journal of General Virology 71:725–729
    [Google Scholar]
  13. Nuss D. L., Dall D. J. 1990; Structural and functional properties of plant reovirus genomes. Advances in Virus Research 38:249–306
    [Google Scholar]
  14. Omura T., Inoue H. 1985; Rice gall dwarf virus. CMI/AAB Descriptions of Plant Viruses no. 296
    [Google Scholar]
  15. Omura T., Morinaka T., Inoue H., Saito Y. 1982; Purification and some properties of rice gall dwarf virus, a new Phytoreovirus. Phytopathology 72:1246–1249
    [Google Scholar]
  16. Omura T., Minobe Y., Matsuoka M., Nozu Y., Tsuchizaki T., Saito Y. 1985; Location of structural proteins in particles of rice gall dwarf virus. Journal of General Virology 66:811–815
    [Google Scholar]
  17. Omura T., Minobe Y., Tsuchizaki T. 1988; Nucleotide sequence of segment S10 of the rice dwarf virus genome. Journal of General Virology 69:227–231
    [Google Scholar]
  18. Omura T., Ishikawa K., Hirano H., Ugaki M., Minobe Y., Tsuchizaki T., Kato T. 1989; The outer capsid protein of rice dwarf virus is encoded by genome segment S8. Journal of General Virology 70:2759–2764
    [Google Scholar]
  19. Reddy D. V. R., MacLeod R. 1976; Polypeptide components of wound tumor virus. Virology 70:274–282
    [Google Scholar]
  20. Streissle G., Granados R. R. 1968; The fine structure of wound tumor virus and reovirus. Archiv fur die Gesamte Virusforschung 25:369–372
    [Google Scholar]
  21. Suzuki N., Watanabe Y., Kusano T., Kitagawa Y. 1990a; Sequence analysis of rice dwarf phytoreovirus genome segments S4, S5 and S6: comparison with the equivalent wound tumor virus segments. Virology 179:446–454
    [Google Scholar]
  22. Sukuki N., Watanabe Y., Kusano T., Kitagawa Y. 1990b; Sequence analysis of the rice dwarf phytoreovirus segment S3 transcript encoding for a major structural core protein of 114 kDa. Virology 179:455–459
    [Google Scholar]
  23. Uyeda I., Shikata E. 1982; Ultrastructure of rice dwarf virus. Annals of the Phytopathological Society of Japan 48:295–300
    [Google Scholar]
  24. Uyeda I., Matsumura T., Sano T., Ohshima K., Shikata T. 1987; Nucleotide sequence of rice dwarf virus genome segment 10. Proceedings of the Japan Academy 63:227–230
    [Google Scholar]
  25. Uyeda I., Kudo H., Takahashi T., Sano T., Ohshima K., Matsumura T., Shikata E. 1989; Nucleotide sequence of rice dwarf virus genome segment 9. Journal of General Virology 70:1297–1300
    [Google Scholar]
  26. Uyeda I., Kudo H., Yamada N., Matsumura T., Shikata E. 1990; Nucleotide sequence of rice dwarf virus genome segment 4. Journal of General Virology 71:2217–2222
    [Google Scholar]
  27. Xu Z., Anzola J. V., Nalin C. M., Nuss D. L. 1989a; The 3′-terminal sequence of a wound tumor virus transcript can influence conformational and functional properties associated with the 5′-terminus. Virology 170:511–522
    [Google Scholar]
  28. Xu Z., Anzola J. V., Nuss D. L. 1989b; Assignment of wound tumor virus nonstructural polypeptides to cognate dsRNA genome segments: in vitro expression of tailored full-length cDNA clones. Virology 168:73–78
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-11-2837
Loading
/content/journal/jgv/10.1099/0022-1317-72-11-2837
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error