%0 Journal Article %A Law, M. D. %A Speck, J. %A Moyer, J. W. %T Nucleotide sequence of the 3′ non-coding region and N gene of the S RNA of a serologically distinct tospovirus %D 1991 %J Journal of General Virology, %V 72 %N 10 %P 2597-2601 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-72-10-2597 %I Microbiology Society, %X A tomato spotted wilt-like virus (TSWV-I) is a distinct member of the Tospovirus genus of the Bunyaviridae and is distinguished from the typical TSWV by having a serologically distinct nucleoprotein (N). A cDNA clone extending from the 3′ terminus of the viral RNA through the entire N open reading frame (ORF) was sequenced. The TSWV-IN ORF is capable of encoding a polypeptide of 262 amino acids with a predicted M r of 28.8K. In vitro transcription and translation of the clone produced a protein which comigrated with TSWV-I N and was immunoprecipitated by TSWV-I antibodies. Hybridization analysis of lithium chloride-precipitated RNA from healthy and TSWV-I-infected tissue detected a virus-specific 1.2 kb subgenomic RNA. The TSWV-I S RNA terminal consensus sequence (8 nucleotides) was identical to that of TSWV; the remaining TSWV-I untranslated region showed only 51% identity with that of TSWV. Comparison of the TSWV-I and TSWV N proteins showed 67% identity at the amino acid level. The degree of similarity in the terminal sequence, untranslated region and N ORF is similar to that expected between distinct serogroups within certain genera of the Bunyaviridae. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-10-2597