RT Journal Article SR Electronic(1) A1 Calvert, Jay G. A1 Nagy, Eva A1 Soler, Maritza A1 Dobos, PeterYR 1991 T1 Characterization of the VPg-dsRNA linkage of infectious pancreatic necrosis virus JF Journal of General Virology, VO 72 IS 10 SP 2563 OP 2567 DO https://doi.org/10.1099/0022-1317-72-10-2563 PB Microbiology Society, SN 1465-2099, AB By the use of strong denaturing agents, a genome-linked protein (VPg)-RNA complex was purified from infectious pancreatic necrosis virus. Ribonuclease treatment of 125I-labelled VPg-RNA released a 90K polypeptide identical to the minor structural polypeptide VP1 (the putative RNA polymerase), as determined by peptide mapping. The polypeptide is linked to the RNA by a serine-5′ GMP phosphodiester bond. The results identify birnaviruses as the only dsRNA viruses with a VPg, the size of which is the largest of the VPgs of RNA viruses., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-10-2563