1887

Abstract

Chimeric polioviruses have been made in which regions of the type 1 Sabin strain corresponding to antigenic sites 2, 3 and 4 have been replaced by the corresponding regions of the type 3 Sabin strain. Manipulation of one site or a component of it generally did not affect the reactions of the others, suggesting that they form independent structural features. The extent to which the inserted site expressed the antigenic properties of type 3 could be assessed by reaction with polyclonal or monoclonal antibodies, or by immunogenicity. Site 2 could be expressed on infectious virus and site 3 on heated non-infectious virus (C antigen), but not on the native virion. The results are consistent with the view that sites consisting of a continuous sequence of amino acids may be presented on chimeras, whereas more complex sites, such as site 4 or site 3 of the native virion, are transferred less readily from type 3 to type 1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-10-2475
1991-10-01
2021-10-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/10/JV0720102475.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-10-2475&mimeType=html&fmt=ahah

References

  1. Blondel B., Crainic R., Fichet O., Dufraisse G., Candrea A., Diamond D., Girard M., Horaud F. 1986; Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibody binding site. Journal of Virology 57:81–90
    [Google Scholar]
  2. Burke K. 1989 Ph.D. thesis University of Reading;
  3. Burke K., Dunn G., Ferguson M., Minor P. D., Almond J. W. 1988; Antigen chimeras of poliovirus as potential new vaccines. Nature, London 332:81–82
    [Google Scholar]
  4. Colbere-Garapin F., Christodoulous C., Crainic R., Garapin A.-C., Candrea A. 1988; Addition of a foreign epitope to the major capsid protein of poliovirus oligopeptide. Proceedings of the National Academy of Sciences, U.S.A 85:8668–8672
    [Google Scholar]
  5. Diamond D. C., Jameson B. A., Bonin J., Kohara M., Abe S., Itoh H., Komatsu T., Arita M., Kuge S., Nomoto A., Osterhaus A. D. M. E., Crainic R., Wimmer E. 1985; Antigenic variation and resistance to neutralization in poliovirus type 1. Science 229:1090–1093
    [Google Scholar]
  6. Evans D. J., McKeating J., Meredith J. M., Burke K. L., Katrak K., John A., Ferguson M., Minor P. D., Weiss R. A., Almond J. W. 1989; An engineered poliovirus chimaera elicits broadly reactive HIV-1 neutralizing antibodies. Nature, London 339:385–388
    [Google Scholar]
  7. Ferguson M., Minor P. D. 1990; Differences in conformation of type 3 poliovirus antigenic sites on non-infectious empty particles and infectious virus. Journal of General Virology 71:1271–1274
    [Google Scholar]
  8. Ferguson M., Minor P. D., Magrath D. I., Qi Y.-H., Spitz M., Schild G. C. 1984; Neutralization epitopes on poliovirus type 3 particles: an analysis using monoclonal antibodies. Journal of General Virology 65:197–201
    [Google Scholar]
  9. Ferguson M., Evans D. M. A., Magrath D. I., Minor P. D., Almond J. W., Schild G. C. 1985; Induction by synthetic peptides of broadly reactive, type specific neutralizing antibody to poliovirus type 3. Virology 143:505–515
    [Google Scholar]
  10. Filman D. J., Syed R., Chow M., Macadam A. J., Minor P. D., Hogle J. M. 1989; Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO Journal 8:1567–1579
    [Google Scholar]
  11. Hogle J. M., Chow M., Filman D. J. 1985; Three dimensional structure of poliovirus at 2.9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  12. Jenkins O., Cason J., Burke K. L., Lunney D., Gillen A., Patel D., McCance D. J., Almond J. W. 1990; An antigen chimera of poliovirus induces antibodies against human papillomavirus type 16. Journal of Virology 64:1201–1206
    [Google Scholar]
  13. Martin A., Wychowski C., Couderc T., Crainic K., Hogle J., Girard M. 1988; Engineering a poliovirus type 2 antigenic site on a type 1 capsid results in a chimaeric virus which is neurovirulent for mice. EMBO Journal 7:2839–2847
    [Google Scholar]
  14. Minor P. D., Schild G. C., Bootman J., Evans D. M. A., Ferguson M., Reeve P., Spitz M., Stanway F., Cann A. J., Hauptmann R., Clarke L. D., Mountford R. C., Almond J. W. 1983; Location and primary structure of a major antigenic site for poliovirus neutralization. Nature, London 301:674–679
    [Google Scholar]
  15. Minor P. D., Evans D. M. A., Ferguson M., Schild G. C., Westrop G., Almond J. W. 1985; Principal and subsidiary antigenic sites of VP1 involved in the neutralization of poliovirus type 3. Journal of General Virology 66:1159–1165
    [Google Scholar]
  16. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  17. Minor P. D., Ferguson M., Katrak K., Wood D., John A., Howlett J., Dunn G., Burke K., Almond J. W. 1990; Antigenic structure of chimeras of type 1 and type 3 poliovirus involving antigenic site 1. Journal of General Virology 71:2543–2551
    [Google Scholar]
  18. Murdin A. D., Wimmer E. 1989; Construction of a poliovirus type 1/type 2 antigenic hybrid by manipulation of neutralization antigenic site II. Journal of Virology 63:5251–5257
    [Google Scholar]
  19. Murray M. G., Kuhn R. J., Arita M., Kawamura N., Nomoto A., Wimmer E. 1988a; Poliovirus type 1/type 3 antigenic hybrid virus contructed in vitro elicits type 1 and type 3 neutralizing antibodies in rabbits and monkeys. Proceedings of the National Academy of Sciences, U.S.A 85:3203–3207
    [Google Scholar]
  20. Murray M. G., Bradley J., Yang X.-F., Wimmer E., Moss E. G., Racaniello V. R. 1988b; Poliovirus host range is determined by a short amino acid sequence in neutralization antigenic site 1. Science 241:213–215
    [Google Scholar]
  21. Page G. S., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow M. 1988; Three dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–1794
    [Google Scholar]
  22. Wiegers K., Uhlig H., Dernick R. 1988; N-AglB of poliovirus type 1: a discontinuous epitope formed by two loops of VP 1 comprising residues 96–104 and 141–152. Virology 70:583–586
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-10-2475
Loading
/content/journal/jgv/10.1099/0022-1317-72-10-2475
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error