1887

Abstract

Four monoclonal antibody-resistant variants (MARVs) of Venezuelan equine encephalitis (VEE) virus were used to study mosquito-virus interactions. experiments using an cell line, C6/36, demonstrated that an amino acid change in the glycoprotein E2 epitope (MARV 1A3B-7) decreased virus growth when compared with the wild-type, Trinidad donkey virus, and its vaccine derivative, TC-83. The MARVs replicated as efficiently as the parent virus when inoculated into mosquitoes, but MARV 1A3B-7 was restricted in its ability to infect and disseminate from the midgut following oral infection. These results demonstrate that a single amino acid change in the E2 glycoprotein can affect the ability of VEE virus to replicate and disseminate in mosquitoes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-10-2431
1991-10-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/10/JV0720102431.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-10-2431&mimeType=html&fmt=ahah

References

  1. Beaty B. J., Bishop D. H. L. 1988; Bunyavirus-vector interactions. Virus Research 10:289–302
    [Google Scholar]
  2. Beaty B. J., Thompson W. H. 1978; Tropisms of La Crosse virus in Aedes triseriatus (Diptera:Culicidae) following infective blood meals. Journal of Medical Entomology 14:499–503
    [Google Scholar]
  3. Beaty B. J., Miller B. R., Shope R. E., Rozhon E. J., Bishop D. H. L. 1982; Molecular basis of bunyavirus per os infection of mosquitoes: role of the middle sized RNA segment. Proceedings of the National Academy of Sciences, U.S.A. 79:1295–1297
    [Google Scholar]
  4. Boggs W. M., Chang S. H., Strauss E. G., Strauss J. H., Griffin D. E. 1989; Low pH-dependent Sindbis virus-induced fusion of BHK cells: differences between strains correlate with amino acid changes in the E1 glycoprotein. Virology 169:485–488
    [Google Scholar]
  5. Dalrymple J. M., Schlesinger S., Russell P. K. 1976; Antigenic characterization of two Sindbis envelope glycoproteins separated by isoelectric focusing. Virology 69:93–103
    [Google Scholar]
  6. Grimstad P. R. 1988; California group virus disease. In The Arboviruses: Epidemiology and Ecology vol 2 pp 99–136 Edited by Monath T. P. Boca Raton: CRC Press;
    [Google Scholar]
  7. Hardy J. L. 1988; Susceptibility and resistance of vector mosquitoes. In The Arboviruses: Epidemiology and Ecology vol 1 pp 87–125 Edited by Monath T. P. Boca Raton: CRC Press;
    [Google Scholar]
  8. Hunt A. R., Johnson A. J., Roehrig J. T. 1990; Synthetic peptides of Venezuelan equine encephalomyelitis virus E2 glycoprotein. I. Immunogenic analysis and identification of a protective peptide. Virology 179:701–711
    [Google Scholar]
  9. Johnson B. J., Brubaker J. R., Roehrig J. T., Trent D. W. 1990; Variants of Venezuelan equine encephalitis virus that resist neutralization define a domain of the E2 glycoprotein. Virology 177:676–683
    [Google Scholar]
  10. Kielian M., Jungerwirth S., Sayad K. U., DeCandido S. 1990; Biosynthesis, maturation and acid activation of the Semliki Forest virus fusion protein. Journal of Virology 64:4614–4624
    [Google Scholar]
  11. Kinney R. M., Esposito J. J., Johnson B. J. B., Roehrig J. T., Mathews J. H., Barrett A. D. T., Trent D. W. 1988; Recombinant vaccinia/Venezuelan equine encephalitis (VEE) virus expresses VEE structural proteins. Journal of General Virology 69:3005–3013
    [Google Scholar]
  12. Monath T. P. 1976; Togaviruses, bunyaviruses, and Colorado tick fever. In Handbook of Clinical Microbiology pp 456–462 Edited by Rose N. R., Friedman H. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  13. Paulson S. L., Grimstad P. R., Craig G. B. Jr 1989; Midgut and salivary gland barriers to La Crosse virus dissemination in mosquitoes of the Aedes triseriatus group. Medical and Veterinary Entomology 3:113–123
    [Google Scholar]
  14. Pedersen C. E. Jr, Robinson D. M., Cole F. E. 1972; Isolation of the vaccine strain of Venezuelan equine encephalomyelitis virus from mosquitoes in Louisiana. American Journal of Epidemiology 95:490–496
    [Google Scholar]
  15. Roehrig J. T., Mathews J. H. 1985; The neutralization site on the E2 glycoprotein of Venezuelan equine encephalomyelitis (TC-83) virus is composed of multiple conformationally stable epitopes. Virology 142:347–356
    [Google Scholar]
  16. Roehrig J. T., Corser J. A., Schlesinger M. J. 1980; Isolation and characterization of hybrid cell lines producing monoclonal antibodies directed against the structural proteins of Sindbis virus. Virology 101:41–49
    [Google Scholar]
  17. Roehrig J. T., Day J. W., Kinney R. M. 1982; Antigenic analyses of the surface glycoproteins of a Venezuelan equine encephalomyelitis virus (TC-83) using monoclonal antibodies. Virology 118:269–278
    [Google Scholar]
  18. Scherer W. F., Cupp E. W., Lok J. B., Brenner R. J., Ordonez J. V. 1981; Intestinal threshold of an enzootic strain of Venezuelan equine encephalitis virus in Culex (Melanoconion) taeniopus mosquitoes and its implications to vector competency and vertebrate amplifying hosts. American Journal of Tropical Medicine and Hygiene 30:862–869
    [Google Scholar]
  19. Scott T. W., Hildreth S. W., Beaty B. J. 1984; The distribution and development of eastern equine encephalitis virus in its enzootic mosquito vector, Culiseta malanura. American Journal of Tropical Medicine and Hygiene 33:300–310
    [Google Scholar]
  20. Strauss J. H., Strauss E. G. 1977; Togaviruses. In The Molecular Biology of Animal Viruses vol 1 pp 111–116 Edited by Nayak D. P. New York: Marcel Dekker;
    [Google Scholar]
  21. Sundin D. R., Beaty B. J., Nathanson N., Gonzalez-Scarano F. 1987; A G1 glycoprotein of La Crosse virus: a determinant of infection of Aedes triseriatus. Science 235:591–593
    [Google Scholar]
  22. Turell M. J. 1988; Horizontal and vertical transmission of viruses by insect and tick vectors. In The Arboviruses: Epidemiology and Ecology vol 4 pp 203–231 Edited by Monath T. P. Boca Raton: CRC Press;
    [Google Scholar]
  23. Weaver S. C., Scott T. W., Lorenz L. H., Lerdthusnee K., Romoser W. S. 1988; Togavirus-associated pathologic changes in the midgut of a natural mosquito vector. Journal of Virology 62:2083–2090
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-10-2431
Loading
/content/journal/jgv/10.1099/0022-1317-72-10-2431
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error