1887

Abstract

During a series of mechanical transfers of tomato spotted wilt virus, two distinct types of mutants were generated. Firstly, a morphologically defective isolate was obtained which had lost the ability to produce the membrane glycoproteins and, as a consequence, was not able to form enveloped particles. Analysis of the genomic RNAs of this isolate suggested that this defect was caused by either point mutations or very small deletions in the medium genomic RNA segment. Secondly, isolates were obtained which had accumulated truncated forms of the large (L) RNA segment. These shortened L RNA molecules most likely represented defective interfering RNAs, since they replicated more rapidly than full-length L RNA and their appearance was often associated with symptom attenuation. Defective L RNAs of different sizes were generated after repeated transfers, and hybridization analysis using L RNA-specific cDNA probes showed that the internal regions deleted varied in length. The presence of defective L RNAs in nucleocapsid fractions as well as in enveloped virus particles indicates that all defective molecules retained the sequences required for replication, encapsidation by nucleocapsid proteins and packaging of the nucleocapsid into virus particles.

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1991-10-01
2022-11-30
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References

  1. Bailey J. M., Davidson N. 1976; Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Analytical Biochemistry 70:75–85
    [Google Scholar]
  2. Black L. M. 1979; Vector-cell monolayers and plant viruses. Advances in Virus Research 25:19–266
    [Google Scholar]
  3. Burgyan J., Grieco F., Russo M. 1989; A defective interfering RNA molecule in cymbidium ringspot virus infections. Journal of General Virology 70:235–239
    [Google Scholar]
  4. Burgyan J., Rubino L., Russo M. 1991; De novo generation of cymbidium ringspot virus defective interfering RNA. Journal of General Virology 72:505–509
    [Google Scholar]
  5. Cascone P. J., Carpenter C. D., Li X. H., Simon A. E. 1990; Recombination between satellite RNAs of turnip crinkle virus. EMBO Journal 9:1709–1715
    [Google Scholar]
  6. Cave D. R., Hendrickson F. M., Huang A. S. 1985; Defective interfering virus particles modulate virulence. Journal of Virology 55:366–373
    [Google Scholar]
  7. Cho J. J., Mau R. F. L., Hamasaki R. T., Gonsalves D. 1988; Detection of tomato spotted wilt virus in individual thrips by enzyme-linked immunosorbent assay. Phytopathology 78:1348–1351
    [Google Scholar]
  8. Cunningham C., Szilagyi J. F. 1987; Viral RNAs synthesized in cells infected with Germiston bunyavirus. Virology 157:431–439
    [Google Scholar]
  9. David-West T. S., Porterfield J. S. 1974; Dugbe virus: a tickborne arbovirus from Nigeria. Journal of General Virology 23:297–307
    [Google Scholar]
  10. de Avila A. C., Huguenot C., Resende R. De. O., Kitajima E. W., Goldbach R., Peters D. 1990; Serological differentiation of 20 isolates of tomato spotted wilt virus. Journal of General Virology 71:2801–2807
    [Google Scholar]
  11. De Haan P., Wagemakers L., Goldbach R., Peters D. 1989a; Tomato spotted wilt virus, a new member of the Bunyaviridae?. In Genetics and Pathogenicity of Negative Strand Viruses pp 287–290 Edited by Kolakofsky D., Mahy B. W. J. Amsterdam: Elsevier;
    [Google Scholar]
  12. De Haan P., Wagemakers L., Peters D., Goldbach R. 1989b; Molecular cloning and terminal sequence determination of the S and the M RNAs of tomato spotted wilt virus. Journal of General Virology 70:3469–3473
    [Google Scholar]
  13. De Haan P., Wagemakers L., Peters D., Goldbach R. 1990; The S RNA segment of tomato spotted wilt virus has an ambisense character. Journal of General Virology 71:1001–1007
    [Google Scholar]
  14. De Haan P., Kormelink R., Resende R. de. O., van Poelwijk D., Peters F., Goldbach R. 1991; Tomato spotted wilt virus L RNA encodes a putative RNA polymerase. Journal of General Virology 72:2207–2216
    [Google Scholar]
  15. De Vries S. C., Springer J., Wessels J. G. H. 1982; Diversity of abundant mRNA sequences and patterns of protein synthesis in etiolated and greened pea seedlings. Planta 156:129–135
    [Google Scholar]
  16. Elliott R. M., Wilkie M. L. 1986; Persistent infection of Aedes albopictus C6/36 cells by Bunyamwera virus. Virology 150:21–32
    [Google Scholar]
  17. Francki R. I. B. 1991; Classification and nomenclature of viruses. Fifth Report of the International Committee of Taxonomy of Viruses.. Archives of Virology (in press)
    [Google Scholar]
  18. Hillman B. I., Carrington J. C., Morris T. J. 1987; A defective interfering RNA that contains a mosaic of plant virus genome. Cell 51:427–433
    [Google Scholar]
  19. Holland J. J. 1986; Generation and replication of defective viral genomes. In Virology pp 77–99 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  20. Huang A. S. 1973; Defective-interfering viruses. Annual Review of Microbiology 27:101–117
    [Google Scholar]
  21. Huguenot C., van den Dobbelsteen G., de Haan P., Wage-makers C. A. M., Drost G. A., Osterhaus A. D. M. E., Peters D. 1990; Detection of tomato spotted wilt virus using monoclonal antibodies and riboprobes. Archives of Virology 110:47–62
    [Google Scholar]
  22. Ie T. S. 1982; A sap-transmissible, defective form of tomato spotted wilt virus. Journal of General Virology 59:387–391
    [Google Scholar]
  23. Ismail I. D., Milner J. J. 1988; Isolation of defective interfering particles of sonchus yellow net virus from chronically infected plants. Journal of General Virology 69:999–1006
    [Google Scholar]
  24. Kascsak R. J., Lyons M. J. 1978; Bunyamwera virus II. The generation and nature of defective interfering particles. Virology 89:539–546
    [Google Scholar]
  25. Knorr D. A., Mullin R. H., Hearne P. Q., Morris A. T. 1991; De novo generation of defective interfering RNAs of tomato bushy stunt virus by high multiplicity passage. Virology 181:193–202
    [Google Scholar]
  26. Lazzarini R. A., Keene J. D., Schubert M. 1981; The origins of defective interfering particles of the negative-strand RNA viruses. Cell 26:145–154
    [Google Scholar]
  27. Li X. H., Heaton L. A., Morris T. J., Simon A. E. 1989; Turnip crinkle virus defective interfering RNAs intensify viral symptoms and are generated de novo. Proceedings of the National Academy of Sciences, U.S.A 86:9173–9177
    [Google Scholar]
  28. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  29. Mohamed N. A., Randles J. W., Francki R. I. B. 1973; Protein composition of tomato spotted wilt virus. Virology 56:12–21
    [Google Scholar]
  30. Morris T. J., Knorr D. A. 1990; Defective interfering viruses associated with plant virus infection. In New Aspects of Positive-Strand RNA Viruses pp 123–127 Edited by Brinton M. A., Heinz F. X. Washington, D. C.: American Society for Microbiology;
    [Google Scholar]
  31. Nayak D. P., Chambers T. M., Akkina R. K. 1985; Defective-interfering (DI) RNAs of influenza viruses: origin, structure, expression and interfering. Current Topics in Microbiology and Immunology 114:103–151
    [Google Scholar]
  32. Nayak D. P., Chambers T. M., Akkina R. K. 1990; Structure of defective interfering RNAs of influenza viruses and their role in interference. In The Influenza Viruses pp 269–317 Edited by Krug R. M. New York: Plenum Press;
    [Google Scholar]
  33. Resende R. de O., de Avila A. C., Kitajima E. W., Goldbach R., Peters D. 1991; Defective isolates of tomato spotted wilt virus. In Virus-Thrips-Plant Interactions of Tomato Spotted Wilt Virus, Proceedings of a USDA Workshop, Beltsville, Maryland April 10–19, 1990, pp 71–76 Edited by Hsu H. T., Lawson R. H. United States Department of Agriculture, Agricultural Research Service;
    [Google Scholar]
  34. Shirako Y., Brakke M. K. 1984; Spontaneous deletion mutation of soil-borne wheat mosaic virus RNA II. Journal of General Virology 65:855–858
    [Google Scholar]
  35. Shirako Y., Ehara Y. 1986; Comparison of the in vitro translation products of wild-type and a deletion mutant of soil-borne wheat mosaic virus. Journal of General Virology 67:1237–1245
    [Google Scholar]
  36. Tas P. W. L., Boerjan M. L., Peters D. 1977; The structural proteins of tomato spotted wilt virus. Journal of General Virology 36:267–279
    [Google Scholar]
  37. van Lent J. W. M., Verduin B. J. M. 1985; Specific gold-labelling of antibodies bound to plant viruses in mixed suspensions. Netherlands Journal of Plant Pathology 91:205–213
    [Google Scholar]
  38. Verani P., Nicoletti L., Marchi A. 1984; Establishment and maintenance of persistent infection by the Phlebovirus Toscana in Vero cells. Journal of General Virology 65:367–375
    [Google Scholar]
  39. Verkleij F. N., Peters D. 1983; Characterization of a defective form of tomato spotted wilt virus. Journal of General Virology 64:677–686
    [Google Scholar]
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