1887

Abstract

The sequence of the fusion (F) glycoprotein of turkey rhinotracheitis virus (TRTV) has been deduced from cDNA clones derived from oligo(dT)-selected infected cell RNA. The protein consists of 538 amino acids, the F2 and F1 subunits containing 102 (including the F2-F1 connecting peptide RRRR) and 436 residues, respectively. Each subunit has one potential -linked glycosylation site. The protein has 38 to 39% amino acid identity with the F protein of respiratory syncytial virus ( genus) but only about half that with members of the other two genera ( and ) in the family. This is the first sequence evidence to support the view that TRTV is a pneumovirus, the first avian member of the genus to be described.

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1991-01-01
2024-12-05
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References

  1. Barrett T., Mahy B. W. J. 1984; Molecular cloning of the nucleoprotein gene of canine distemper virus. Journal of General Virology 65:549–557
    [Google Scholar]
  2. Barrett T., Clarke D. K., Evans S. A., Rima B. K. 1987; The nucleotide sequence of the gene encoding the F protein of canine distemper virus: a comparison of the deduced amino acid sequence with other paramyxoviruses. Virus Research 8:373–386
    [Google Scholar]
  3. Baybutt H. N., Pringle C. R. 1987; Molecular cloning and sequencing of the F and 22K membrane protein genes of the RSS-2 strain of respiratory syncytial virus. Journal of General Virology 68:2789–2796
    [Google Scholar]
  4. Binns M. M., Boursnell M. E. G., Cavanagh D., Pappin D. J. C., Brown T. D. K. 1985; Cloning and sequencing of the gene encoding the spike protein of the coronavirus IBV. Journal of General Virology 66:719–726
    [Google Scholar]
  5. Buckland R., Gerald C., Barker R., Wild T. F. 1987; Fusion glycoprotein of measles virus: nucleotide sequence of the gene and comparison with other paramyxoviruses. Journal of General Virology 68:1695–1703
    [Google Scholar]
  6. Buys S. B., du Preez J. H., Els H. J. 1989; The isolation and attenuation of a virus causing rhinotracheitis in turkeys in South Africa. Onderstepoort Journal of Veterinary Research 56:87–98
    [Google Scholar]
  7. Cavanagh D., Barrett T. 1988; Pneumovirus-like characteristics of the mRNA and proteins of turkey rhinotracheitis virus. Virus Research 11:241–256
    [Google Scholar]
  8. Chambers P., Millar N. S., Emmerson P. T. 1986; Nucleotide sequence of the gene encoding the fusion glycoprotein of Newcastle disease virus. Journal of General Virology 67:2685–2694
    [Google Scholar]
  9. Chambers P., Barr J., Pringle C. R., Easton A. J. 1990; Molecular cloning of pneumonia virus of mice. Journal of Virology 64:1869–1872
    [Google Scholar]
  10. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  11. Collins M. S., Gough R. E. 1988; Characterization of a virus associated with turkey rhinotracheitis. Journal of General Virology 69:909–916
    [Google Scholar]
  12. Collins P. L., Huang Y. T., Wertz G. W. 1984a; Identification of a tenth mRNA of respiratory syncytial virus and assignment of polypeptides to the 10 viral genes. Journal of Virology 49:572–578
    [Google Scholar]
  13. Collins P. L., Huang Y. T., Wertz G. W. 1984b; Nucleotide sequence of the gene encoding the fusion (F) glycoprotein of human respiratory syncytial virus. Proceedings of the National Academy of Sciences, U.S.A 81:7683–7687
    [Google Scholar]
  14. Collins P. L., Dickens L. E., Buckler-White A., Olmsted R. A., Spriggs M. K., Camargo E., Coelingh K. V. W. 1986; Nucleotide sequences for the gene junctions of human respiratory syncytial virus reveal distinctive features of intergenic structure and gene order. Proceedings of the National Academy of Sciences, U.S.A 83:4594–4598
    [Google Scholar]
  15. Dayhoff M. O., Eck R. V., Park C. M. 1972; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure vol 5 pp. 89–99 Edited by Dayhoff M. O. Washington, D.C.: National Biomedical Research Foundation;
    [Google Scholar]
  16. Elango N., Satake M., Coligan J. E., Norrby E., Camargo E., Venkatesan S. 1985; Respiratory syncytial virus fusion glycoprotein: nucleotide sequence of mRNA, identification of cleavage activation site and amino acid sequence of N-terminus F1 subunit. Nucleic Acids Research 13:1559–1574
    [Google Scholar]
  17. Evans S. A., Belsham G. J., Barrett T. 1990; The role of the 5ʹ nontranslated regions of the fusion protein mRNAs of canine distemper virus and rinderpest virus. Virology 177:317–323
    [Google Scholar]
  18. Hubbard S. C., Ivatt R. J. 1981; Synthesis and processing of asparagine-linked oligosaccharides. Annual Review of Biochemistry 50:555–583
    [Google Scholar]
  19. Johnson P. R., Collins P. L. 1988; The fusion glycoproteins of human respiratory syncytial virus of subgroups A and B: sequence conservation provides a structural basis for antigenic relatedness. Journal of General Virology 69:2623–2628
    [Google Scholar]
  20. Jones R. C., Baxter-Jones C., Wilding D., Kelly F. 1986; Demonstration of a candidate virus for turkey rhinotracheitis in experimentally inoculated turkeys. Veterinary Record 119:599–600
    [Google Scholar]
  21. Ling R., Pringle C. R. 1988; Turkey rhinotracheitis virus: in vivo and in vitro polypeptide synthesis. Journal of General Virology 69:917–923
    [Google Scholar]
  22. Ling R., Pringle C. R. 1989; Polypeptides of penumonia virus of mice. I. Immunological cross-reactions and post-translational modifications. Journal of General Virology 70:1427–1440
    [Google Scholar]
  23. Lopez J. A., Villanueva N., Melero J. A., Portela A. 1988; Nucleotide sequence of the fusion and phosphoprotein genes of human respiratory syncytial (RS) virus Long strain: evidence of subtype genetic heterogeneity. Virus Research 10:249–262
    [Google Scholar]
  24. McDougall J. S., Cook J. K. A. 1986; Turkey rhinotracheitis: preliminary investigations. Veterinary Record 118:206–207
    [Google Scholar]
  25. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Miura N., Ohtsuka E., Yamaberi N., Ikehara M., Uchida T., Okada Y. 1985; Use of the deoxyinosine-containing probe to isolate and sequence cDNA encoding the fusion (F) glycoprotein of Sendai virus (HVJ). Gene 38:271–274
    [Google Scholar]
  27. Morrison T. G. 1988; Structure, function, and intracellular processing of paramyxovirus membrane proteins. Virus Research 10:113–136
    [Google Scholar]
  28. Murphy G., Kavanagh T. 1988; Speeding-up the sequencing of double-stranded DNA. Nucleic Acids Research 16:5198
    [Google Scholar]
  29. Paterson R. G., Harris T. J. R., Lamb R. A. 1984; Fusion protein of the paramyxovirus simian virus 5: nucleotide sequence of the mRNA predicts a highly hydrophobic glycoprotein. Proceedings of the National Academy of Sciences, U.S.A 81:6706–6710
    [Google Scholar]
  30. Picault J. P., Giraud P., Drouin P., Guittet M., Bennejean G., Lamande J., Toquin D., Guegeun C. 1987; Isolation of a TRTV-like virus from chickens with swollen-head syndrome. Veterinary Record 121:135
    [Google Scholar]
  31. Richardson C., Hull D., Greer P., Hasel K., Berkovich A., Englund G., Bellini W. J., Rima B. K., Lazzarini R. 1986; The nucleotide sequence of the mRNA encoding the fusion protein of measles virus (Edmonston strain): a comparison of fusion proteins from several different paramyxoviruses. Virology 155:508–523
    [Google Scholar]
  32. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  33. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequence of the F, HN and L proteins. Nucleic Acids Research 14:1545–1563
    [Google Scholar]
  34. Spriggs M. K., Olmsted R. A., Venkatesan S., Coligan J. E., Collins P. L. 1986; Fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. Virology 152:241–251
    [Google Scholar]
  35. Suzu S., Sakai Y., Shioda T., Shibuta H. 1987; Nucleotide sequence of the bovine parainfluenza 3 virus genome: the genes of the F and HN glycoproteins. Nucleic Acids Research 15:2945–2958
    [Google Scholar]
  36. Tsukiyama K., Yoshikawa Y., Yamanouchi K. 1988; Fusion glycoprotein (F) of rinderpest virus: entire nucleotide sequence of the mRNA, and several features of the F protein. Virology 164:523–530
    [Google Scholar]
  37. Waxham M. N., Server A. C., Goodman H. W., Wolinsky J. S. 1987; Cloning and sequencing of the mumps virus fusion protein gene. Virology 159:381–388
    [Google Scholar]
  38. Wyeth P. J., Gough R. E., Chettle N., Eddy R. 1986; Preliminary observations on a virus associated with turkey rhinotracheitis. Veterinary Record 119:139
    [Google Scholar]
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