RT Journal Article SR Electronic(1) A1 Walker, Peter J. A1 Byrne, Keren A. A1 Cybinski, Daisy H. A1 Doolan, Denise L. A1 Wang, YonghongYR 1991 T1 Proteins of Bovine Ephemeral Fever Virus JF Journal of General Virology, VO 72 IS 1 SP 67 OP 74 DO https://doi.org/10.1099/0022-1317-72-1-67 PB Microbiology Society, SN 1465-2099, AB The proteins of bovine ephemeral fever virus (BEFV) were examined in purified virions and in infected BHK-21 cells. Five structural proteins were named L (180K), G (81K), N (52K), M1 (43K) and M2 (29K). The 81 K G protein incorporated [3H]glucosamine, was removed from virions by treatment with Triton X-100 and bound monoclonal antibodies which were both neutralizing and protective. Treatment of virions with Triton X-100 and 0.2 to 1.0 m-NaCl progressively released L, M1 and M2. The N protein remained associated with nucleocapsids in up to 2.5 m-NaCl. The glycoprotein (G), nucleoprotein (N) and matrix protein (M2) were phosphorylated. In BEFV-infected BHK-21 cells, five virus-induced proteins were detected from 12 h post-infection. The L, N, M1 and M2 proteins corresponded to those detected in virions whereas the G protein existed in two forms. In tunicamycin-treated cells these occurred as 67K and 71K non-glycosylated precursors. In the absence of tunicamycin, 77K and 79K glycosylated forms were further modified to produce the 81K virion G protein and a 90K cell-associated form. Five viral proteins were also detected in cells infected with the closely related Berrimah virus; the Berrimah virus G protein was also present in two forms., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-1-67