%0 Journal Article %A Osman, T. A. M. %A Miller, S. J. %A Marriott, A. C. %A Buck, K. W. %T Nucleotide Sequence of RNA 2 of a Czechoslovakian Isolate of Red Clover Necrotic Mosaic Virus %D 1991 %J Journal of General Virology, %V 72 %N 1 %P 213-216 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-72-1-213 %I Microbiology Society, %X The complete nucleotide sequence (1448 nucleotides) of RNA 2 of a Czechoslovakian isolate TpM-34 of red clover necrotic mosaic virus (RCNMV-TpM-34) has been determined. The sequence contained one major open reading frame (ORF) with the potential to encode a protein of 326 amino acids (Mr 35755), designated P2. The nucleotide sequence of RNA 2 of RCNMV-TpM-34 and the previously published sequence of RNA 2 of an Australian isolate of the virus (RCNMV-Aus) were 83% identical and there was 80% amino acid sequence identity between the P2 proteins of these isolates. However the N-terminal two-thirds of the P2 proteins shared a higher degree of similarity than the C-terminal regions which were predicted to have a more flexible structure. An ORF in the 3′ portion of RNA 2 of RCNMV-Aus, which could encode a protein of M r 5000, was not present in RNA 2 of RCNMV-TpM-34. RNAs 1 and 2 of RCNMV-TpM-34 and RCNMV-Aus are bilaterally compatible. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-72-1-213