1887

Abstract

RNA prepared from the potyvirus tobacco vein mottling virus contained aggregates of the 9.5 kb genomic RNA with electrophoretic mobilities corresponding to 20 and 41 kb species. Similar aggregates were present in preparations of the RNAs of two other potyviruses. Aggregation occurred during or after purification of the RNA by sucrose gradient centrifugation and alcohol precipitation and was dependent upon the presence of a protein apparently bound covalently to a region at or near the 5′ terminus of the viral RNA. This protein is probably the VPg. The RNAs of tobacco mosaic virus and cowpea mosaic virus did not form aggregates when isolated from purified virus by similar procedures.

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1991-01-01
2024-04-24
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References

  1. Boedtker H., Simmons N. S. 1958; The preparation and characterization of essentially uniform tobacco mosaic virus particles. Journal of the American Chemical Society 80:2550–2557
    [Google Scholar]
  2. Calvert L. A., Ghabrial S. A. 1983; Enhancement by soybean mosaic virus of bean pod mottle virus titer in doubly infected soybean. Phytopathology 73:992–997
    [Google Scholar]
  3. Domier L. L., Franklin K. M., Shahabuddin M., Hellmann G. M., Overmyer J., Hiremath S. T., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  4. Hari V., Siegel A., Rozek C., Timberlake W. E. 1979; The RNA of tobacco etch virus contains poly(A). Virology 92:568–571
    [Google Scholar]
  5. Hellmann G. M., Shaw J. G., Lesnaw J. A., Chu L.-Y., Pirone T. P., Rhoads R. E. 1980; Cell-free translation of tobacco vein mottling virus RNA. Virology 106:207–216
    [Google Scholar]
  6. Hellmann G. M., Shahabuddin M., Shaw J. G., Rhoads R. E. 1983; Molecular cloning of DNA complementary to tobacco vein mottling virus RNA. Virology 128:210–220
    [Google Scholar]
  7. Kunkel T. A., Roberts J. D., Zakour R. A. 1987; Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods in Enzymology 154:367–382
    [Google Scholar]
  8. Loening U. E., Ingle J. 1967; Diversity of RNA components in green plant tissue. Nature, London 213:363–367
    [Google Scholar]
  9. McMaster K. G., Carmichael G. G. 1977; Analysis of single and double-stranded nucleic acids on polyacrylamide and agarose gels using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U,. S,. A 74:4835–4838
    [Google Scholar]
  10. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Moghal S. M., Francki R. I. B. 1976; Towards a system for the identification of potyviruses. I. Serology and amino acid composition of six distinct viruses. Virology 73:350–362
    [Google Scholar]
  12. Murphy J. F., Rhoads R. E., Hunt A. G., Shaw J. G. 1990; The VPg of tobacco etch virus RNA is the 49 kDa proteinase or the N-terminal 24 kDa part of the proteinase. Virology 178:285–288
    [Google Scholar]
  13. Riechmann J. L., Laín S., García J. A. 1989; The genome-linked protein and 5′ end RNA sequence of plum pox potyvirus. Journal of General Virology 70:2785–2789
    [Google Scholar]
  14. Siaw M., Shahabuddin M., Ballard S., Shaw J. G., Rhoads R. E. 1985; Identification of a protein covalently linked to the 5′ terminus of tobacco vein mottling virus RNA. Virology 142:134–143
    [Google Scholar]
  15. Stanley J., van Kammen A. 1979; Nucleotide sequences adjacent to the proteins covalently linked to the cowpea mosaic virus genome. European Journal of Biochemistry 101:45–49
    [Google Scholar]
  16. Stanley J., Rottier P., Davies J. W., Zabel P., van Kammen A. 1978; A protein covalently linked to the 5′ termini of both RNA components of the cowpea mosaic virus genome. Nucleic Acids Research 12:4505–4522
    [Google Scholar]
  17. Thomas P. S. 1983; Hybridization of denatured RNA transferred or dotted to nitrocellulose paper. Methods in Enzymology 100:255–266
    [Google Scholar]
  18. Van Kammen A., de Jager C. P. 1978; Cowpea mosaic virus. CMI/AAB Descriptions of Plant Viruses no. 197
    [Google Scholar]
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