1887

Abstract

Influenza A (H1N1) and influenza B viruses from clinical samples were isolated in the amniotic cavity of embryonated hens’ eggs by classical techniques and propagated in the allantoic cavity. Virus progeny from different eggs which had been inoculated with virus material from the same clinical sample possessed antigenically distinguishable haemagglutinins (HAs). Virus progeny of some eggs possessed HAs which were serologically identical to those of virus isolated in parallel in mammalian (MDCK) cells. These egg-grown viruses possessing HAs with the antigenic phenotype of mammalian cell-grown viruses appeared to be antigenically related to epidemic influenza virus because post-infection human sera reacted to high titre with the virus HA. Specific nucleotide changes were detected in the HAs of the viruses isolated directly in eggs at positions 163 and 189 for influenza A (H1Na) viruses or positions 141 and 196 to 198 for influenza B viruses. Egg-isolated viruses which possessed the antigenic phenotype of mammalian cell-grown viruses retained glycosylation sites at positions 163 and 196. The viruses isolated directly in embryonated hens’ eggs which possessed the HA antigenic phenotype and glycosylation sites of MDCK cell-grown virus can, unlike the latter viruses themselves, be used as candidate influenza vaccine viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-72-1-185
1991-01-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/72/1/JV0720010185.html?itemId=/content/journal/jgv/10.1099/0022-1317-72-1-185&mimeType=html&fmt=ahah

References

  1. Burnet F. M., Clarke E. 1942 Walter and Eliza Hall Institute, Monograph No. 4 Melbourne: Macmillan;
    [Google Scholar]
  2. Caton A. J., Brownlee G. G., Yewdell J. W., Gerhard W. 1982; The antigenic structure of the influenza virus A/PR/8/34 haemagglutinin (HI subtype). Cell 31:417–427
    [Google Scholar]
  3. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., van de Pol S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  4. Hoskins T. W., Davies J. R., Smith A. J., Miller C., Allchin A. 1979; Assessment of inactivated influenza A vaccine after three outbreaks of influenza A at Christ’s Hospital. Lancet i:33–35
    [Google Scholar]
  5. Krystal M., Elliott R. M., Benz E. W., Young J. F., Palese P. 1982; Evolution of influenza A and B viruses: conservation of structural features in the hemagglutinin genes. Proceedings of the National Academy of Sciences, U.S.A. 79:4800–4804
    [Google Scholar]
  6. Oxford J. S., Abbo H., Corcoran T., Webster R. G., Smith A. J., Grilli E. A., Schild G. C. 1983; Antigenic and biochemical analysis of field isolates of influenza B virus: evidence for intra- and inter-epidemic variation. Journal of General Virology 64:2367–2377
    [Google Scholar]
  7. Oxford J. S., Corcoran T., Knott R., Bates J., Bartolomei O., Major D., Newman R. W., Robertson J. S., Webster R. G., Schild G. C. 1987; Serological studies with influenza A (H1N1) viruses cultivated in eggs or in a canine kidney cell line (MDCK). Bulletin of the World Health Organization 65:181–187
    [Google Scholar]
  8. Oxford J. S., Schild G. C., Corcoran T., Newman R., Major D., Robertson J. S., Bootman J. S., Higgins P. G., Nakib W. A., Barrow I., Tyrrell D. A. J. 1990; A host cell selected variant of influenza B virus with a single nucleotide substitution in HA affecting a potential glycosylation site was attenuated in virulence for volunteers. Archives of Virology 110:37–46
    [Google Scholar]
  9. Patterson S., Oxford J. S. 1986; Analysis of antigenic determinants of internal and external proteins of influenza virus and identification of antigenic subpopulations of virions in recent field isolates using monoclonal antibodies and immunogold labelling. Archives of Virology 88:189–202
    [Google Scholar]
  10. Robertson J. S., Naeve C. W., Webster R. G., Bootman J. S., Newman R., Schild G. C. 1985; Alterations in the hemagglutinin associated with adaptation of influenza B virus to growth in eggs. Virology 143:166–174
    [Google Scholar]
  11. Robertson J. S., Bootman J. S., Oxford J. S., Daniels R. S., Webster R. G., Schild G. C. 1987; Structural changes in the hemagglutinin which accompany egg adaptation of an influenza A (H1N1) virus. Virology 160:31–37
    [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  13. Schild G. C., Oxford J. S., de Jong J. C., Webster R. G. 1983; Evidence of host cell selection of influenza virus antigenic variants. Nature, London 303:706–709
    [Google Scholar]
  14. Stuart-Harris C. H., Schild G. C., Oxford J. S. 1985 Influenza. The Viruses and the Disease London: Edward Arnold;
    [Google Scholar]
  15. Wang M., Katz J. M., Webster R. G. 1989; Extensive heterogeneity in the hemagglutinin of egg-grown influenza viruses from different patients. Virology 171:275–279
    [Google Scholar]
  16. Wiley D. C., Skehel J. J. 1987; The structure and function of the hemagglutinin membrane glycoprotein of influenza virus. Annual Review of Biochemistry 56:365–394
    [Google Scholar]
  17. Wood J. M., Oxford J. S., Dunleavy V., Newman R. W., Major D., Robertson J. S. 1989; Influenza A (H1N1) vaccine efficacy in animal models is influenced by two amino acid substitutions in the hemagglutinin molecule. Virology 171:214–221
    [Google Scholar]
  18. Zuckerman M., Wood J., Taylor J., Oxford J. S. 1990; The influenza A (H3N2) component of vaccine induces antibody to the current virus.. Lancet i:179
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-72-1-185
Loading
/content/journal/jgv/10.1099/0022-1317-72-1-185
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error