1887

Abstract

A number of independent cDNA clones of the Piry virus N gene message were identified and sequenced. From the resulting sequences and previously published data, we derived the sequence of the mRNA for this protein. Sequence similarities of the translated region of Piry virus with that of other viruses suggest that Piry virus is as distantly related to Chandipura virus as it is to the vesicular stomatitis viruses of Indiana and New Jersey serotypes. Based on the relative conservation of the amino acid sequence of the nucleocapsid protein of these vesiculoviruses, the N protein can be subdivided into at least three regions, possibly indicative of functional domains.

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1990-09-01
2024-04-24
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References

  1. Banerjee A. K., Rhodes D. P., Gill D. S. 1984; Complete nucleotide sequence of the mRNA coding for the N protein of vesicular stomatitis virus (New Jersey serotype). Virology 137:432–438
    [Google Scholar]
  2. Bell J. C., Prevec L. 1979; Proteins of vesicular stomatitis virus. V. Identification of a precursor to the phosphoprotein of Piry virus. Journal of Virology 30:56–63
    [Google Scholar]
  3. Brown E. G., Prevec L. 1979; Comparative analysis of vesiculovirus proteins utilizing partial cleavage fragments at tryptophan residues. Virology 95:244–248
    [Google Scholar]
  4. Cartwright B., Brown F. 1972; Serological relationships between different strains of vesicular stomatitis virus. Journal of General Virology 16:391–398
    [Google Scholar]
  5. Chou P. Y., Fasman G. D. 1974; Prediction of protein conformation. Biochemistry 13:222–245
    [Google Scholar]
  6. Crick J., Brown F. 1973; Interference as a measure of crossrelationships in the vesicular stomatitis group of rhabdo viruses. Journal of General Virology 18:79–82
    [Google Scholar]
  7. Gallione C. J., Greene J. R., Iverson L. E., Rose J. K. 1981; Nucleotide sequences of the mRNA’s encoding the vesicular stomatitis virus N and NS proteins. Journal of Virology 39:529–535
    [Google Scholar]
  8. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  9. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  10. Masters P. S., Banerjee A. K. 1987; Sequences of Chandipura virus N and NS genes: evidence for high mutability of the NS gene within vesiculoviruses. Virology 157:298–306
    [Google Scholar]
  11. Nichol S. T., Holland J. J. 1987; Genome RNA terminus conservation and diversity among vesiculoviruses. Journal of Virology 61:200–205
    [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  13. Taylor R. M. 1967 Complete Catalog of Arthropod Borne Viruses of the World U.S. Public Health Service, Publication No. 1760, U.S. Government Printing Office, Washington, D.C:
    [Google Scholar]
  14. Thomas D., Newcomb W. W., Brown J. C., Wall J. S., Hainfeld J. F., Trus B. S., Steven A. C. 1985; Mass and molecular composition of vesicular stomatitis virus: a scanning transmission electron microscopy analysis. Journal of Virology 54:598–607
    [Google Scholar]
  15. Wilbur W. J., Lipman D. J. 1983; Rapid similarity searches of nucleic acid and protein data banks. Proceedings of the National Academy of Sciences, U.S.A 80:726–730
    [Google Scholar]
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