We have studied the genetic relationships of echoviruses using nucleotide sequencing and hybridization analysis. The nucleotide sequence of the echovirus 11 (EV11) P2 and P3 regions, which encode the nonstructural proteins, was shown to resemble closely those of coxsackie B viruses (CBV) and coxsackievirus A9 (CAV9). EV11, CBV and CAV9 have a similar organization in the 3′ non-coding region when compared to polioviruses and CAV21. In contrast, the 3′ end of EV22 shares only minimal sequence homology with other sequenced enteroviruses, and the 3′ non-coding region has a unique secondary structure. Thirty-three echovirus reference strains were tested by nucleic acid hybridization using cDNA probes from the genomes of EV6, 11, 18 and 22. It was shown that a great majority of the strains belongs to the same subgroup as serotypes 6, 11 and 18, whereas EV22 and EV23 are genetically not closely related to this major subgroup.
ArgosP.,
KamerG.,
NicklinM. J. H.,
WimmerE.1984; Similarity in gene organization and homology between proteins of animal picornaviruses and plant comovirus suggest common ancestry of these virus families. Nucleic Acids Research 12:7251–7267
BazanJ. F.,
FletterickR. J.1988; Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications. Proceedings of the National Academy of Sciences. U.S.A 85:7872–7876
ChangK. H.,
AuvinenP.,
HyypiäT.,
StanwayG.1989; The nucleotide sequence of coxsackievirus A9; implications for receptor binding and enterovirus classification. Journal of General Virology 70:3269–3280
DeverT. E.,
GlyniasM. J.,
MerrickW. C.1987; GTP-binding domain: three consensus sequence elements with distinct spacing. Proceedings of the National Academy of Sciences, U.S.A 84:1814–1818
GrunsteinM.,
HognessD. S.1975; Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proceedings of the National Academy of Sciences, U.S.A 72:3961–3965
HughesP. J.,
NorthC.,
JellisC. H.,
MinorP. D.,
StanwayG.1988; The nucleotide sequence of human rhinovirus IB: molecular relationships within the rhinovirus genus. Journal of General Virology 69:49–58
JenkinsO.,
BoothJ. D.,
MinorP. D.,
AlmondJ. W.1987; The complete nucleotide sequence of coxsackievirus B4 and its comparison to other members of the picornaviridae. Journal of General Virology 68:1835–1848
KamerG.,
ArgosP.1984; Primary structural comparison of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7282
NajarianR.,
CaputD.,
GeeW.,
PotterS. J.,
RenardA.,
MerryweatherJ.,
NestG. V.,
DinaD.1985; Primary structure and gene organization of human hepatitis A virus. Proceedings of the National Academy of Sciences, U.S.A 82:2627–2631
PochO.,
SauvagetI.,
DelarueM.,
TordoN.1989; Identification of four conserved motifs among the RNA-dependent polymerase encoding elements. EMBO Journal 8:3867–3874
SarnowP.,
BernsteinH. D.,
BaltimoreD.1986; A poliovirus temperature-sensitive RNA synthesis mutant located in a noncoding region of the genome. Proceedings of the National Academy of Sciences. U.S.A 83:571–575
TaborS.,
RichardsonC. C.1987; DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences, U.S.A 84:4767–4771
ToyodaH.,
NicklinM. J. H.,
MurrayM. G.,
AndersonC. W.,
DunnJ. J.,
StudierF. W.,
WimmerE.1986; A second virus-encoded proteinase involved in proteolytic processing of poliovirus polyprotein. Cell 45:761–770
WernerG.,
RosenwirthB.,
BauerE.,
SeifertJ.-M.,
WernerF.-J.,
BesemerJ.1986; Molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses. Journal of Virology 57:1084–1093
ZagurskyR. J.,
BaumeisterK.,
LomaxN.,
BermanM. L.1985; Rapid and easy sequencing of large linear double-stranded DNA and supercoiled plasmid DNA. Gene Analysis Techniques 2:89–94