1887

Abstract

We have partially cloned and sequenced the genome of a Peruvian yellow fever virus isolate (1899/81) and compared the nucleotide and deduced amino acid sequences of this strain with the previously published sequence of the West African yellow fever virus strain Asibi. In the 3594 base region sequenced, which contains the structural genes (C, M, E), all but the 72 3′-terminal nucleotides of the NS1 gene and 108 nucleotides of the 5′ non-coding region, 515 nucleotide substitutions were detected. Nucleotide divergence was lowest in the 5′ non-coding region, 2·8%, compared with an average rate of 14·7% in the coding regions. Over 91 % of the 512 nucleotide changes in the coding region were silent; 44 amino acid substitutions resulted. The capsid protein was the least conserved, whereas the M protein was the most highly conserved (6·7% and 1·3% divergence, respectively). The envelope protein had 18 amino acid changes (3·7% divergence), one of which created an additional site for potential glycosylation of the 1899/81 virus. NS1 protein divergence (3·9%) was similar to that seen in the E protein. Of the 44 amino acid substitutions found, 34 (77%) were conservative. The highest number of nonconservative differences occurred in the envelope glycoprotein. These changes may significantly affect the antigenic and biological functions of the viruses.

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1990-09-01
2024-04-19
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References

  1. Cane P. A., Gould E. A. 1989; Immunoblotting reveals differences in the accumulation of envelope protein by wild-type and vaccine strains of yellow fever virus. Journal of General Virology 70:557–564
    [Google Scholar]
  2. Carter H. R. 1931 Yellow Fever: An Epidemiological and Historical Study of Its Place of Origin Baltimore: Williams & Wilkins Co;
    [Google Scholar]
  3. Clarke D. H. 1960; Antigenic analysis of certain group B arthropod- borne viruses by antibody absorption. Journal of Experimental Medicine 111:21–32
    [Google Scholar]
  4. Dale R. M. K., Mcclure B. A., Houchins J. P. 1985; A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18 rDNA. Plasmid 13:31–40
    [Google Scholar]
  5. Deubel V., Digoutte J.-P., Monath T. P., Girard M. 1986; Genetic heterogeneity of yellow fever virus strains from Africa and the Americas. Journal of General Virology 67:209–213
    [Google Scholar]
  6. Deubel V., Schlesinger J. J., Digoutte J.-P., Girard M. 1987; Comparative immunochemical and biological analysis of African and South American yellow fever viruses. Archives of Virology 94:331–338
    [Google Scholar]
  7. Hahn C. S., Dalrymple J. M., Strauss J. H., Rice C. M. 1987; Comparison of the virulent Asibi strain of yellow fever virus with the 17D vaccine strain derived from it. Proceedings of the National Academy of Sciences, U.S.A 84:2019–2023
    [Google Scholar]
  8. Heinz F. X. 1986; Epitope mapping of flavivirus glycoproteins. Advances in Virus Research 31:103–186
    [Google Scholar]
  9. Holland J., Spindler K., Horodyski F., Grabau E., Nichol S., VandePol S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  10. Kinney R. M., Johnson B. J. B., Brown V. L., Trent D. W. 1986; Nucleotide sequence of the 26S mRNA of the virulent Trinidad donkey strain of Venezuelan equine encephalitis virus and deduced sequence of the encoded structural proteins. Virology 152:400–413
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 121680–685
    [Google Scholar]
  12. Mandl C. W., Guirakhoo F., Holzmann H., Heinz F. X., Kunz C. 1989; Antigenic structure of the flavivirus envelope protein E at the molecular level, using tick-borne encephalitis virus as a model. Journal of Virology 63:564–571
    [Google Scholar]
  13. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Mendez M. R., Calisher C. H., Kruger H., Sipan F., Sanchez S., Lazuick J. S. 1984; A continuing focus of yellow fever in the Apurimac River valley, Ayacucho, Peru, and the first isolation of yellow fever virus in that country. Bulletin of the Pan American Health Organization 18:172–179
    [Google Scholar]
  15. Miller B. R., Adkins D. 1988; Biological characterization of plaque-size variants of yellow fever virus in mosquitoes and mice. Actavirologica 32:227–234
    [Google Scholar]
  16. Nowak T., Wengler G. 1987; Analysis of disulfides present in the membrane proteins of the West Nile flavivirus. Virology 156:127–137
    [Google Scholar]
  17. Reanney D. C. 1982; The evolution of RNA viruses. Annual Review of Microbiology 36:47–73
    [Google Scholar]
  18. Rice C. M., Lenches E. M., Eddy S. R., Shin S. R., Sheets R. L., Strauss J. H. 1985; Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution. Science 229:726–733
    [Google Scholar]
  19. Roehrig J. T. 1986; The use of monoclonal antibodies in studies of the structural proteins of togaviruses and flaviviruses. In The Togaviridae and Flaviviridae pp. 251–278 Schlesinger S., Schlesinger M. J. Edited by New York: Plenum Press;
    [Google Scholar]
  20. Roehrig J. T., Hunt A. R., Johnson A. J., Hawkes R. A. 1989; Synthetic peptides derived from the deduced amino acid sequence of the E-glycoprotein of Murray Valley encephalitis virus elicit antiviral antibody. Virology 171:49–60
    [Google Scholar]
  21. Ruiz-Linares A., Bouloy M., Girard M., Cahour A. 1989; Modulations of the in vitro translational efficiencies of yellow fever virus mRNAs: interactions between coding and noncoding regions. Nucleic Acids Research 17:2463–2476
    [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  23. Schlesinger J. J., Brandriss M. W., Monath T. P. 1983; Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition, and immune precipitation of the virus envelope protein. Virology 125:8–17
    [Google Scholar]
  24. Strauss J. H., Strauss E. G. 1988; Evolution of RNA viruses. Annual Review of Microbiology 42:657–683
    [Google Scholar]
  25. Taylor R. M. 1951; Epidemiology. In Yellow Fever pp. 529–533 Strode G. K. Edited by New York: McGraw-Hill;
    [Google Scholar]
  26. Trent D. W., Clewley J. P., France J. K., Bishop D. H. L. 1979; Immunochemical and oligonucleotide fingerprint analyses of Venezuelan equine encephalomyelitis complex viruses. Journal of General Virology 43:365–381
    [Google Scholar]
  27. Wechsler S. L., Rustigian R., Stallcup K. C., Myers K. B., Winston S. H., Fields B. N. 1979; Measles virus-specified polypeptide synthesis in two persistently infected HeLa cell lines. Journal of Virology 31:677–684
    [Google Scholar]
  28. Westaway E. G. 1980; Replication of flaviviruses. In The Togaviruses pp. 531–581 Schlesinger R. W. Edited by New York: Academic Press;
    [Google Scholar]
  29. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequence of the M13 mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
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