1887

Abstract

The antigenic structures of the envelope protein, E, and the non-structural protein, NS1, of dengue type 1 virus (DENI) have been studied in the form of recombinant fusion proteins expressed in . Deletion analysis was used to identify two distinct antigenic domains in E that reacted with subsets of antiviral monoclonal antibodies (MAbs). Domain I of E extends from amino acid residues (aa) 76 to 93 of E; domain II extends from aa 293 to 402 and contains an essential disulphide bridge. MAbs also reacted with several determinants clustered near the N terminus of the NS 1 protein (aa 57 to 126). Recombinant fusion proteins containing sequences and most of the sequences for either E or NS1 were immunogenic in mice. The antibodies elicited by the E fusion protein reacted with a portion of the protein containing domain II, whereas antibodies elicited by the NS1 fusion protein did not react with the antigenic determinants defined by our MAbs.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-9-2107
1990-09-01
2023-01-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/9/JV0710092107.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-9-2107&mimeType=html&fmt=ahah

References

  1. Brandt W. E., Buescher E. L., Hetrick F. M. 1967; Production and characterization of arbovirus antibody in mouse ascitic fluid. American Journal of Tropical Medicine and Hygiene 16:339–347
    [Google Scholar]
  2. Dieckmann C. L., Tzagoloff A. 1985; Assembly of the mitochondrial membrane system. CBP6, a yeast nuclear gene necessary for synthesis of cytochrome b. Journal of Biological Chemistry 260:1513–1520
    [Google Scholar]
  3. Engvall E. 1980; Enzyme immunoassay ELISA and EMIT. Methods in Enzymology 70:419–439
    [Google Scholar]
  4. Gentry M. K., Henchal E. A., Mccown J. M., Brandt W. E., Dalrymple J. M. 1982; Identification of distinct antigenic determinants on Dengue-2 virus using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 31:548–555
    [Google Scholar]
  5. Halstead S. B. 1988; Pathogenesis of dengue: challenges to molecular biology. Science 239:476–481
    [Google Scholar]
  6. Harlow E., Lane D. 1988 Antibodies: A Laboratory Manual pp. 236–237 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. Henchal E. A., Gentry M. K., Mccown J. M., Brandt W. E. 1982; Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. American Journal of Tropical Medicine and Hygiene 31:830–836
    [Google Scholar]
  8. Henchal E. A., Mccown J. M., Burke D. S., Seguin M. C., Brandt W. E. 1985; Epitopic analysis of antigenic determinants on the surface of dengue-2 virions using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 34:162–169
    [Google Scholar]
  9. Kaufman B. M., Summers P. L., Dubois D. R., Eckels K. H. 1987; Monoclonal antibodies against dengue 2 virus E-glycoprotein protect mice against lethal dengue infection. American Journal of Tropical Medicine and Hygiene 36:427–434
    [Google Scholar]
  10. Lobigs M., Dalgarno L., Schlesinger J. J., Weir R. C. 1987; Location of a neutralizing determinant in the E protein of yellow fever virus (17D vaccine strain). Virology 161:474–478
    [Google Scholar]
  11. Mandl C. W., Guirakhoo F., Holzmann H., Heinz F. X., Kunz C. 1989; Antigenic structure of the flavivirus envelope protein E at the molecular level, using tick-borne encephalitis virus as a model. Journal of Virology 63:564–571
    [Google Scholar]
  12. Mason P. W., Mcada P. C., Dalrymple J. M., Fournier M. J., Mason T. L. 1987a; Expression of Japanese encephalitis virus antigens in Escherichia coli . Virology 158:361–372
    [Google Scholar]
  13. Mason P. W., Mcada P. C., Mason T. L., Fournier M. J. 1987b; Sequence of the dengue-1 virus genome in the region encoding the three structural proteins and the major non-structural protein NS1. Virology 161:262–267
    [Google Scholar]
  14. Mason P. W., Dalrymple J. M., Gentry M. K., Mccown J. M., Hoke C. H., Burke D. S., Fournier M. J., Mason T. L. 1989; Molecular characterization of a neutralizing domain of the Japanese encephalitis virus structural glycoprotein. Journal of General Virology 70:2037–2049
    [Google Scholar]
  15. Monath T. P. 1986; Pathobiology of the flaviviruses. In The Togaviridae and Flaviviridae pp. 375–440 Schlesinger S., Schlesinger M. J. Edited by New York & London: Plenum Press;
    [Google Scholar]
  16. Monath T. P., Wands J. R., Hill L. J., Brown N. V., Marciniak R. A., Wong M. A., Gentry M. K., Burke D. S., Grant J. A., Trent D. W. 1986; Geographic classification of dengue-2 virus strains by antigen signature analysis. Virology 154:313–324
    [Google Scholar]
  17. Morens D. M., Halstead S. B., Repik P. M., Putvatana R., Raybourne N. 1985; Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization. Journal of Clinical Microbiology 22:250–254
    [Google Scholar]
  18. Nowak T., Wengler G. 1987; Analysis of disulfides present in the membrane proteins of the West Nile flavivirus. Virology 156:127–137
    [Google Scholar]
  19. Putnak J. R., Charles P. C., Padmanabhan R., Irie K., Hoke C. H., Burke D. S. 1988; Functional and antigenic domains of the dengue-2 virus nonstructural glycoprotein NS-1. Virology 163:93–103
    [Google Scholar]
  20. Russell P. K., Nisalak A. 1967; Dengue virus identification by the plaque reduction neutralization test. Journal of Immunology 99:291–296
    [Google Scholar]
  21. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  22. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  23. Schlesinger J. J., Brandriss M. W., Walsh E. E. 1987; Protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein NS1. Journal of General Virology 68:853–857
    [Google Scholar]
  24. Shope R. E. 1980; Medical significance of togaviruses: an overview of diseases caused by togaviruses in man and in domestic and wild vertebrate animals. In The Togaviruses pp. 47–82 Schlesinger R. W. Edited by New York & London: Academic Press;
    [Google Scholar]
  25. Tesh R. B. 1979; A method for the isolation and identification of dengue viruses, using mosquito cell cultures. American Journal of Tropical Medicine and Hygiene 28:1053–1059
    [Google Scholar]
  26. Tsuji T., Nakagawa R., Sugimoto N., Fukuhara K. 1987; Characterization of disulfide bonds in recombinant proteins: reduced human interleukin 2 in inclusion bodies and its oxidative refolding. Biochemistry 26:3129–3134
    [Google Scholar]
  27. Winkler G., Heinz F. X., Kunz C. 1987; Characterization of a disulphide bridge-stabilized antigenic domain of tick-borne encephalitis virus structural glycoprotein. Journal of General Virology 68:2239–2244
    [Google Scholar]
  28. Wright P. J., Cauchi M. R., Ng M. L. 1989; Definition of the carboxy termini of the three glycoproteins specified by dengue virus type 2. Virology 171:61–67
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-9-2107
Loading
/content/journal/jgv/10.1099/0022-1317-71-9-2107
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error