1887

Abstract

Monoclonal antibodies (MAbs) were produced against the G, M2 and N proteins of bovine ephemeral fever virus (BEFV) and 29 were selected for further study. Thirteen neutralizing MAbs were assigned to one conformation-independent and at least two conformation-dependent antigenic sites on the G protein by a competitive binding ELISA. The panel of MAbs were tested by neutralization and immunofluorescence with three strains of BEFV and three BEFV-related viruses. The results indicated that BEFV strains from different sources were not identical and that the M2 protein was the least variable of the proteins investigated. Passive protection studies in mice showed that the correlation between neutralizing titre and resistance to challenge was 0· 85 ( < 0· 001).

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-9-2065
1990-09-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/9/JV0710092065.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-9-2065&mimeType=html&fmt=ahah

References

  1. Brodeur B. R., Tsang P., Larose Y. 1984; Parameters affecting ascites tumour formation in mice and monoclonal antibody production. Journal of Immunological Methods 71:265–272
    [Google Scholar]
  2. Bruck C., Portetelle D., Glineur C., Bollen A. 1982; One-step purification of mouse monoclonal antibodies from ascites fluid by DEAE Affi-gel blue chromatography. Journal of Immunological Methods 53:313–319
    [Google Scholar]
  3. Bunschoten H., Gore M., Claassen I. TH. M., Uytdehaag F. G. C. M., Dietzschold B., Wunner W. H., Osterhaus A. D. M. E. 1989; Characterization of a new virus-neutralizing epitope that denotes a sequential determinant on the rabies virus glycoprotein. Journal of General Virology 70:291–298
    [Google Scholar]
  4. Calisher C. H., Karabatsos N., Zeller H., Digoutte J., Tesh R. B., Shope R. E., Travassos Da Rosa A. P. A., St George T. D. 1989; Antigenic relationships among rhabdoviruses from vertebrates and hematophagous arthropods. Intervirology 50:241–257
    [Google Scholar]
  5. Cecilia D., Gadkari D. A., Kedarnath N., Ghosh S. N. 1988; Epitope mapping of Japanese encephalitis virus envelope protein using monoclonal antibodies against an Indian strain. Journal of General Virology 69:2741–2747
    [Google Scholar]
  6. Cybinski D. H. 1987; Homologous and heterologous antibody reactions in sera from cattle naturally infected with bovine ephemeral fever group viruses. Veterinary Microbiology 13:1–9
    [Google Scholar]
  7. Cybinski D. H., Zakrzewski H. 1983; The isolation and preliminary characterization of a rhabdovirus in Australia related to bovine ephemeral fever virus. Veterinary Microbiology 8:221–235
    [Google Scholar]
  8. Cybinski D. H., St George T. D., Paull N. I. 1978; Antibodies to Akabane virus in Australia. Australian Veterinary Journal 54:1–3
    [Google Scholar]
  9. Della-Porta A. J., Brown F. 1979; The physico-chemical characterization of bovine ephemeral fever virus as a member of the family Rhabdoviridae. Journal of General Virology 44:99–112
    [Google Scholar]
  10. Della-Porta A. J., Snowdon W. A. 1979; An experimental inactivated virus vaccine against bovine ephemeral fever. 2. Do neutralizing antibodies protect against infection?. Veterinary Microbiology 4:197–208
    [Google Scholar]
  11. Doherty R. L., Standfast H. A., Clark I. A. 1969; Adaptation to mice of the causative agent of ephemeral fever in cattle from an epizootic in Queensland, 1968. Australian Journal of Science 31:365–366
    [Google Scholar]
  12. Flamand A., Wiktor T. J., Koprowski H. 1980; Use of hybridoma monoclonal antibodies in the detection of antigenic differences between rabies and rabies-related virus proteins. I. The nucleocapsid protein. Journal of General Virology 48:97–104
    [Google Scholar]
  13. Gard G. P., Cybinski D. H., St George T. D. 1983; The isolation in Australia of a new virus related to bovine ephemeral fever virus. Australian Veterinary Journal 60:89–90
    [Google Scholar]
  14. Gard G. P., Cybinski D. H., Zakrzewski H. 1984; The isolation of a fourth bovine ephemeral fever group virus. Australian Veterinary Journal 61:332
    [Google Scholar]
  15. Gretch D. R., Suter M., Stinski M. F. 1987; The use of biotinylated monoclonal antibodies and streptavidin affinity chromatography to isolate herpes virus hydrophobic proteins or glycoproteins. Analytical Biochemistry 163:270–277
    [Google Scholar]
  16. Hughes G., Babiuk L. A., van Drunen Littel-van den Hurk S. 1988; Functional and topographical analysis of epitopes on bovine herpesvirus type 1 glycoprotein IV. Archives of Virology 103:47–60
    [Google Scholar]
  17. Kearney J. F., Radbruch A., Fiesegang B., Rajewsky K. 1979; A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. Journal of Immunology 123:1548–1550
    [Google Scholar]
  18. Köhler G., Milstein D. 1976; Derivation of specific antibody- producing tissue culture and tumour lines by cell fusion. European Journal of Immunology 6:511–519
    [Google Scholar]
  19. Lafon M., Wiktor T. J., Macfarlan R. I. 1983; Antigenic sites on the CVS rabies virus glycoprotein: analysis with monoclonal antibodies. Journal of General Virology 64:843–851
    [Google Scholar]
  20. Murphy F. A., Taylor W. P., Mims C. A., Whitfield S. G. 1972; Bovine ephemeral fever virus in cell culture and mice. Archiv für die gesamte Virusforschung 38:234–239
    [Google Scholar]
  21. Redinbaugh M. G., Campbell W. H. 1985; Adaptation of the dye-binding protein assay to microtitre plates. Analytical Biochemistry 147:144–147
    [Google Scholar]
  22. Sartorelli A. C., Fischer D. S., Downs W. G. 1966; Use of sarcoma 180/TG to prepare hyperimmuneascitic fluid in the mouse. Journal of Immunology 96:676–682
    [Google Scholar]
  23. Snowdon W. A. 1970; Bovine ephemeral fever: the reaction of cattle to different strains of ephemeral fever virus and the antigenic comparison of two strains of virus. Australian Veterinary Journal 46:258–266
    [Google Scholar]
  24. Standfast H. A., St George T. D., Dyce A. L. 1976; The isolation of ephemeral fever virus from mosquitoes in Australia. Australian Veterinary Journal 52:242
    [Google Scholar]
  25. Wiktor T. J., Gyorgy E., Schlumberger H. D., Sokol F., Koprowski H. 1973; Antigenic properties of rabies virus components. Journal of Immunology 110:269–276
    [Google Scholar]
  26. Wiktor T. J., Lafon M., Dietzschold B., Wunner W. 1983; Recognition of antigenic variants of rabies virus by monoclonal antibodies. In Advances in Gene Technology: Molecular Genetics of Plants and Animals pp. 279–284 Downey K., Voellmy R. W., Ahmad F., Schultz J. Edited by San Diego: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-9-2065
Loading
/content/journal/jgv/10.1099/0022-1317-71-9-2065
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error