Recombination of genomic terminus of bovine herpesvirus type 1 with cellular DNA Free

Abstract

Bovine herpesvirus 1 (BHV-1) has a linear DNA genome of about 135 kb which appears as two isomers, resulting from its short unique segment being present in the two possible orientations with respect to the large unique segment. BHV-1 also circularizes its DNA to form replicative molecules. Definition of the target sequences at the genomic termini involved in the recombination events during genomic replication and isomerization, as well as virus maturation, led to the discovery that 10% of the genome molecules have additional DNA sequences attached to the right-hand terminus, as shown by electron microscopy. Three such tails have been cloned molecularly; they differ in length and nucleotide sequence, and hybridization experiments demonstrate the cellular origin of two of the three tails. The evidence presented here is consistent with a proportion of the BHV-1 genomes recombining their DNA with cellular DNA during lytic infection.

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1990-09-01
2024-03-29
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References

  1. Anvret M., Karlsson A., Bjursell G. 1984; Evidence for integrated EBV genomes in Raji cellular DNA. Nucleic Acids Research 12:1149–1161
    [Google Scholar]
  2. Ben-Porat T., Rixon F. J., Blankenship M. L. 1979; Analysis of the structure of the genome of pseudorabies virus. Virology 95:285–294
    [Google Scholar]
  3. Dambaugh T., Beisel C., Hummel M., King W., Fennewald S., Cheung A, Raab-Traub N., Kieff E. 1980; Epstein-Barr virus (B95-8) DNA. VII: Molecular cloning and detailed mapping. Proceedings of the National Academy of Sciences, U.S.A 77:2999–3003
    [Google Scholar]
  4. Davies D. H., Carmichael L. E. 1973; Role of cell-mediated immunity in the recovery of cattle from primary and recurrent infections with infectious bovine rhinotracheitis virus. Infection and Immunity 8:510–518
    [Google Scholar]
  5. Davison A. J., Scott J. E. 1986; The complete sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  6. Ecker J. R., Kudler L., Hyman R. W. 1984; Variation in the structure of varicella-zoster virus DNA. Intervirology 21:25–37
    [Google Scholar]
  7. Efstathiou S., Minson A. C., Field H. J., Anderson J. R., Wildy P. 1986; Detection of herpes simplex virus-specific DNA sequences in latently infected mice and in humans. Journal of Virology 57:446–455
    [Google Scholar]
  8. Fraser N. W., Lawrence W. C., Wroblewska Z., Gilden D. H., Koprowski H. 1981; Herpes simplex type 1 DNA in human brain tissue. Proceedings of the National Academy of Sciences, U.S.A 78:6461–6465
    [Google Scholar]
  9. Hammerschmidt W., Ludwig H., Buhk H.-J. 1986; Short repeats cause heterogeneity at genomic terminus of bovine herpesvirus 1. Journal of Virology 58:43–49
    [Google Scholar]
  10. Hammerschmidt W., Ludwig H., Buhk H.-J. 1988; Specificity of cleavage in replicative form DNA of bovine herpesvirus type 1. Journal of Virology 62:1355–1363
    [Google Scholar]
  11. Heller M., Henderson A., Kieff E. 1982; Repeat array in Epstein-Barr virus DNA is related to cell DNA sequences interspersed on human chromosomes. Proceedings of the National Academy of Sciences, U.S.A 79:5916–5920
    [Google Scholar]
  12. Heller M., Flemington E., Kieff E., Deininger P. 1985; Repeat arrays in cellular DNA related to the Epstein-Barr virus IR3 repeat. Molecular and Cellular Biology 5:457–465
    [Google Scholar]
  13. Henderson A., Ripley S., Heller M., Kieff E. 1983; Chromosome site for Epstein-Barr virus DNA in a Burkitt tumor cell line and in lymphocytes growth-transformed in vitro . Proceedings of the National Academy of Sciences, U.S.A 80:1987–1991
    [Google Scholar]
  14. Hirai K., Ikuta K., Kitamoto N., Kato S. 1981; Latency of herpesvirus of turkey and Marek’s disease virus genomes in a chicken T-lymphoblastoid cell line. Journal of General Virology 53:133–143
    [Google Scholar]
  15. Hu N., Messing J. 1982; The making of strand-specific M13 probes. Gene 17:271–277
    [Google Scholar]
  16. Jansen H. W., Ruckert B., Lurz R., Bister K. 1983; Two unrelated cell-derived sequences in the genome of avian leukemia and carcinoma inducing retrovirus MH2. EMBO Journal 2:1969–1975
    [Google Scholar]
  17. Kaschka-Dierich C., Werner F. J., Bauer I., Fleckenstein B. 1982; Structure of nonintegrated, circular herpesvirussaimiri and herpesvirusateles genomes in tumor cell lines and in vitro-transformed cells. Journal of Virology 44:295–310
    [Google Scholar]
  18. Lawrence J. B., Villnave C. A., Singer R. H. 1988; Sensitive, high-resolution chromatin and chromosome mapping in situ: presence and orientation of two closely integrated copies of EBV in a lymphoma cell line. Cell 52:51–61
    [Google Scholar]
  19. Ludwig H., Storz J. 1973; Activation of herpesvirus from normal bovine fetal spleen cells after prolonged cultivation. Medical Microbiology and Immunology 158:209–217
    [Google Scholar]
  20. Matsuo T., Heller M., Petti L., O’Shiro E., Kieff E. 1984; Persistence of the entire Epstein-Barr virus genome integrated into human lymphocyte DNA. Science 226:1322–1324
    [Google Scholar]
  21. Maxam A. M., Gilbert W. 1977; A new method for sequencing DNA. Proceedings of the National Academy of Sciences, U.S.A 74:560–564
    [Google Scholar]
  22. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  23. Mellerick D. M., Fraser N. W. 1987; Physical state of the latent herpes simplex genome in a mouse model system: evidence suggesting an episomal state. Virology 158:265–275
    [Google Scholar]
  24. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–78
    [Google Scholar]
  25. Puga A., Cantin E. M., Wohlenberg C., Openshaw H., Notkins A. L. 1984; Different sizes of restriction endonuclease fragments from the terminal repetitions of the herpes simplex virus type 1 genome latent in trigeminal ganglia of mice. Journal of General Virology 65:437–444
    [Google Scholar]
  26. Richardson K. K., Crosby R. M., Good P. J., Rosen N. L., Mayfield J. E. 1986; Bovine DNA contains a single major family of interspersed repetitive sequences. European Journal of Biochemistry 154:349–354
    [Google Scholar]
  27. Rock D. L., Fraser N. W. 1983; Detection of HSV-1 genome in central nervous system of latently infected mice. Nature; London: 302523–525
    [Google Scholar]
  28. Rock D. L., Fraser N. W. 1985; Latent herpes simplex virus type 1 DNA contains two copies of the virion DNA joint region. Journal of Virology 55:849–852
    [Google Scholar]
  29. Rziha H.-J., Bauer B. 1982; Circular forms of viral DNA in Marek’s disease virus-transformed lymphoblastoid cells. Archives of Virology 72:211–216
    [Google Scholar]
  30. Rziha H.-J., Mettenleitner T. C., Ohlinger V., Wittmann G. 1986; Herpesvirus (pseudorabies virus) latency in swine: occurrence and physical state of viral DNA in neural tissues. Virology 155:600–613
    [Google Scholar]
  31. Sanger F., Coulson A. R., Barrell B. G., Smith A. J. H., Roe B. A. 1980; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  32. Sheffy B. E., Davies D. H. 1972; Reactivation of a bovine herpes virus after corticoidsteroid treatment. Proceedings of the Society for Experimental Biology and Medicine 140:974–976
    [Google Scholar]
  33. Snowdown W. A. 1965; The IBR-IPV virus: reaction to infection and intermittent recovery of virus from experimentally infected cattle. Australian Veterinary Journal 41:135–142
    [Google Scholar]
  34. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  35. Tanaka A., Silver S., Nonoyama M. 1978; Biochemical evidence of the nonintegrated status of Marek’s disease virus DNA in virus-transformed lymphoblastoid cells of chicken. Virology 88:19–24
    [Google Scholar]
  36. Toussaint A., Resibois A. 1983; Phage Mu: transposition as a lifestyle. In Mobile Genetic Elements pp. 105–158 Shapiro J. Edited by New York: Academic Press;
    [Google Scholar]
  37. Weber P. C., Challberg M. D., Nelson N. J., Levine M., Glorioso J. C. 1988; Inversion events in the HSV-1 genome are directly mediated by the viral DNA replication machinery and lack sequence specificity. Cell 54:369–381
    [Google Scholar]
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