1887

Abstract

Proteins of purified virions of equine herpesvirus 4 (EHV-4; equine rhinopneumonitis), EHV-1 (equine abortion virus) and asinine herpesvirus 3 (AHV-3) were compared by metabolic labelling with [S]methionine or [C]glucosamine during growth of low passage virus in natural host cells (horse or donkey) and high passage virus in an appropriate cell line and analysis by SDS-PAGE. Approximately 25 different proteins ( 300K to 21·5K) were clearly resolved for each virus. The three viruses had similar profiles although significant differences were found. The proteins of the cell line-grown viruses were similar to their precursor viruses grown in natural host cells although some small differences, probably related to differences in glycosy- lation by the various cell types, were noted. Six or seven high abundance glycoproteins were identified for EHV- 4, EHV-1 and AHV-3. The profile of seven glycoproteins of AHV-3 was more similar to EHV-1 than to EHV-4. Antigenic relationships of the proteins of the three viruses were examined using radioimmunopreci- pitation (RIP) and Western blot analyses and a series of polyclonal sera raised in colostrum-deprived, specific pathogen-free (SPF) foals which were immunized with inactivated EHV-4 (foal 3) or EHV-1 (foal 1), challenged and cross-challenged; a polyclonal donkey serum to AHV-3 was also used. The ontogeny of the antibody response in the SPF foals was studied and the major immunogenic proteins, as determined by RIP, were correlated with previously determined serum neutralizing antibody titres. Antibodies were first detected 14 days after primary immunization and were directed to EHV-4 proteins of , 113K, 75K and 56K or EHV-1 proteins of 110K, 78K, 60K and 58K. Antibodies to these same three (EHV-4) or four (EHV- 1) proteins, together with antibodies to the major capsid protein and proteins of 67K (EHV-4) and 87K (EHV-1) were detected in response to primary infection (control foal 2) and these sera had high neutralizing antibody titres. The antigens of the three viruses were extensively cross-reactive with immunodominant proteins in the ranges 150K to 11 OK and 62K to 56K. However, cross-absorption of EHV-4 and EHV-1 SPF foal antisera indicated the presence of significant amounts of type-specific antibody.

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1990-09-01
2024-03-29
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References

  1. Allen G. P., Bryans J. T. 1986; Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus-1 infections. Progress in Veterinary Microbiology and Immunology 2:78–144
    [Google Scholar]
  2. Allen G. P., Coogle L. D. 1988; Characterization of an equine herpesvirus type 1 gene encoding a glycoprotein (gpl3) with homology to herpes simplex virus glycoprotein C. Journal of Virology 62:2850–2858
    [Google Scholar]
  3. Allen G. P., Turtinen L. W. 1982; Assessment of base sequence homology between the two subtypes of equine herpesvirus 1. Journal of Virology 44:249–255
    [Google Scholar]
  4. Allen G. P., Yeargan M. R. 1987; Use of lambda gt11 and monoclonal antibodies to map the genes for the six major glycoproteins of equine herpesvirus 1. Journal of Virology 61:2454–2461
    [Google Scholar]
  5. Allen G. P., Yeargan M. R., Coogle L. D. 1987; Equid herpesvirus-1 glycoprotein 13 (gpl3): epitope analysis, gene structure, and expression in E. coli . In Equine Infectious Diseases. V, Proceedings of the Fifth International Conference on Equine Infectious Diseases pp. 103–110 Powell D. G. Edited by Lexington: University Press of Kentucky;
    [Google Scholar]
  6. Browning G. F., Ficorilli N., Studdert M. J. 1988; Asinine herpesvirus genomes: comparison with those of the equine herpesviruses. Archives of Virology 101:183–190
    [Google Scholar]
  7. Campbell T. M., Studdert M. J. 1983; Equine herpesvirus type 1 (EHV-1). Veterinary Bulletin 53:135–146
    [Google Scholar]
  8. Cullinane A. A., Rixon F. J., Davison A. J. 1988; Characterization of the genome of equine herpesvirus 1 subtype 2. Journal of General Virology 69:1575–1590
    [Google Scholar]
  9. Doll E. R., Bryans J. T. 1963; Epizootiology of equine viral rhinopneumonitis. Journal of American Veterinary Medical Association 142:31–37
    [Google Scholar]
  10. Fitzpatrick D. R., Studdert M. J. 1984; Immunologic relationships between equine herpesvirus type 1 (equine abortion virus) and type 4 (equine rhinopneumonitis virus). American Journal of Veterinary Research 45:1947–1952
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  12. Marsden H. S. 1987; Herpes simplex virus glycoproteins and pathogenesis. In Molecular Basis of Viral Disease, Society for General Microbiology Symposium 40 pp. 259–288 Russell W. C., Almond J. W. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  13. Meredith D. M., Stocks J.-M., Whittaker G. R., Halliburton I. W., Snowden B. W., Killington R. A. 1989; Identification of the gB homologues of equine herpesvirus types 1 and 4 as disulphide-linked heterodimers and their characterization using monoclonal antibodies. Journal of General Virology 70:1161–1172
    [Google Scholar]
  14. Norrild B. 1985; Humoral responses to HSV infections. In The Herpesviruses 4 pp. 69–86 Roizman B., Lopez C. Edited by New York: Plenum Press;
    [Google Scholar]
  15. O’Callaghan D. J., Randall C. C. 1976; Molecular anatomy of herpesviruses: recent studies. Progress in Medical Virology 22:152–210
    [Google Scholar]
  16. O’Shannessy D. J., Voorstad P. J., Quarles R. H. 1987; Quantitation of glycoproteins on electroblots using the biotin-streptavidin complex. Analytical Biochemistry 163:204–209
    [Google Scholar]
  17. Perdue M. L., Kemp M. C., Randall C. C., O’Callaghan D. J. 1974; Studies of the molecular anatomy of the L-M cell strain of equine herpes virus type 1: proteins of the nucleocapsid and intact virion. Virology 59:201–216
    [Google Scholar]
  18. Sabine M., Robertson G. R., Whalley J. M. 1981; Differentiation of the subtypes of equine herpesvirus 1 by restriction endonuclease analysis. Equine Veterinary Journal 57:148–149
    [Google Scholar]
  19. Schrag J. D., Prasad B. V. V., Rixon F. J., Chiu W. 1989; Three-dimensional structure of the HSV-1 nucleocapsid. Cell 56:651–660
    [Google Scholar]
  20. Studdert M. J. 1983; Restriction endonuclease DNA fingerprinting of respiratory, foetal and perinatal foal isolates of equine herpesvirus type 1. Archives of Virology 77:249–258
    [Google Scholar]
  21. Studdert M. J., Blackney M. H. 1979; Equine herpesviruses: on the differentiation of respiratory from foetal strains of type 1. Australian Veterinary Journal 55:488–492
    [Google Scholar]
  22. Studdert M. J., Simpson T., Roizman B. 1981; Differentiation of respiratory and abortigenic isolates of equine herpesvirus 1 by restriction endonucleases. Science 214:562–564
    [Google Scholar]
  23. Sullivan D. C., Allen G. P., O’Callaghan D. J. 1989; Synthesis and processing of equine herpesvirus type 1 glycoprotein 14. Virology 173:638–646
    [Google Scholar]
  24. Turtinen L. W. 1983 Studies of the antigenic and genetic variation between the two subtypes of equine herpesvirus 1 Ph.D. thesis University of Kentucky, Lexington:
    [Google Scholar]
  25. Turtinen L. W., Allen G. P., Darlington R. W., Bryans J. T. 1981; Serologic and molecular comparisons of several equine herpesvirus type 1 infections. American Journal of Veterinary Research 42:2099–2104
    [Google Scholar]
  26. Vestergaard B. F. 1980; Herpes simplex virus antigens and antibodies. A survey of studies based on quantitative immunoelectrophoresis. Review of Infectious Diseases 2:899–913
    [Google Scholar]
  27. Yeargan M. R., Allen G. P., Bryans J. T. 1985; Rapid subtyping of equine herpesvirus 1 with monoclonal antibodies. Journal of Clinical Microbiology 21:694–697
    [Google Scholar]
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