1887

Abstract

Infection of tissue culture cells with vaccinia virus results in the specific secretion of several polypeptides into the medium. Previous studies identified a protein of approximate 35000 (35K) which was secreted in large amounts at both early and late times after infection with the Evans strain. We now show that a related protein is secreted by the Lister strain but not by WR, Wyeth nor Tian Tan. The gene encoding the Lister strain 35K protein was mapped within the inverted terminal repeats of the genome. The DNA sequence of this region showed that the ends of this gene are very similar to previously published sequences flanking a gene of WR which encodes a protein of approximate 7500 (7·5K). Our results suggest that the 7·5K polypeptide of WR may have arisen as a result of a deletion event and is a truncated form of the 35K Lister protein. Site-directed mutagenesis demonstrated that the 35K secreted protein encoded by Lister is not essential for growth in tissue culture.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-9-2013
1990-09-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/9/JV0710092013.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-9-2013&mimeType=html&fmt=ahah

References

  1. Bankier A. T., Weston K. M., Barrell B. G. 1987; Random cloning and sequencing by the M13/dideoxynucleotide chain termination method. methods in enzymology 155:51–93
    [Google Scholar]
  2. Biggin M. D., Gibson T. J., Hong G. F. 1983; Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings Of The National Academy Of Sciences, U.S.A. 80:3963–3965
    [Google Scholar]
  3. Chakrabarti S., Brechling K., Moss B. 1985; Vaccinia virus expression vector: co-expression of β-galactosidase provides visual screening of recombinant virus plaques. molecular and cellular biology 5:3403–3409
    [Google Scholar]
  4. Cochran M. A., Puckett C., Moss B. 1985; in vitro mutagenesis of the promoter region of a vaccinia virus gene: evidence of tandem early and late regulatory signals. journal of virology 54:30–37
    [Google Scholar]
  5. Cooper J. A., Wittek R., Moss B. 1981; Hybridization selection and cell-free translation of mRNAs encoded within the inverted terminal repetition of the vaccinia virus genome. journal of virology 37:284–294
    [Google Scholar]
  6. Davison A. J., Moss B. 1989; Structure of vaccinia virus early promoters. journal of molecular biology 210:749–769
    [Google Scholar]
  7. Esposito J., Knight J. C. 1985; Orthopoxvirus DNA: a comparison of restriction profiles and maps. virology 143:230–251
    [Google Scholar]
  8. Garon C. F., Barbosa E., Moss B. 1978; Visualization of an inverted terminal repetition in vaccinia virus DNA. Proceedings Of The National Academy Of Sciences, U.S.A. 75:4863–4867
    [Google Scholar]
  9. Geshelin P., Berns K. I. 1974; Characterization and localization of the naturally occurring crosslinks in vaccinia virus DNA. Journal Of Molecular Biology 88:785–796
    [Google Scholar]
  10. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18
    [Google Scholar]
  11. Kotwal G. J., Moss B. 1988; Vaccinia virus encodes a secretory polypeptide structurally related to complement control proteins. Nature; London: 335176–178
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227:680–685
    [Google Scholar]
  13. McCrae M. A., Pennington T. H. 1978; Specific secretion of polypeptides from cells infected with vaccinia virus. journal of virology 28:828–834
    [Google Scholar]
  14. Mackett M., Archard L. C. 1979; Conservation and variation in orthopoxvirusgenome structure. journal of general virology 45:683–701
    [Google Scholar]
  15. Mackett M., Smith G. L., Moss B. 1984; General method for production and selection of infectious vaccinia virus recombinants expressing foreign genes. journal of virology 49:857–864
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Moss B. 1985; Replication of poxviruses. In Virology pp. 685–703 Fields B. N. Edited by New York: Raven Press;
    [Google Scholar]
  18. Moss B., Winters E., Cooper N. 1981; Instability and reiteration of DNA sequence within the vaccinia virus genome. Proceedings Of The National Academy Of Sciences, U.S.A. 78:1614–1618
    [Google Scholar]
  19. Paterson B. M., Roberts B. E., Kuff E. L. 1977; Structural gene identification and mapping by DNA-mRNA hybrid-arrested cell-free translation. Proceedings of the National Academy of Sciences, U.S.A 74:4370–4374
    [Google Scholar]
  20. Pearson W. R., Lipman D. J. 1988; Improved tools for biological comparison. Proceedings of the National Academy of Sciences, U.S.A 85:2444–2448
    [Google Scholar]
  21. Pickup D. J., Bastia D., Stone H. O., Joklik W. K. 1982; Sequence of terminal regions of cowpox virus DNA: arrangement of repeated and unique sequence elements. Proceedings of the National Academy of Sciences, U.S.A 79:7112–7116
    [Google Scholar]
  22. Rosel J., Earl P., Weir J. P., Moss B. 1986; Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment. Journal of Virology 60:436–449
    [Google Scholar]
  23. Sanger F., Coulson A. R., Barrell B. J., Smith J. H., Roe B. A. 1977; Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing. Journal of Molecular Biology 143:161–178
    [Google Scholar]
  24. Schümperli D., Menna A., Schwendimann F., Wlttek R., Wyler R. 1980; Symmetrical arrangement of the heterologous regions of rabbit poxvirus and vaccinia virus DNA. Journal of General Virology 47:385–398
    [Google Scholar]
  25. Smith G. L., Chan Y. S., Kerr S. M. 1989; Transcriptional mapping and nucleotide sequence of a vaccinia virus gene encoding a polypeptide with extensive homology to DNA ligase. Nucleic Acids Research 17:9051–9062
    [Google Scholar]
  26. Staden R. 1982; Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Research 10:4731–4751
    [Google Scholar]
  27. Stroobant P., Rice A. P., Gullick W. J., Chang D. J., Kerr I. M., Waterfield M. D. 1985; Purification and characterization of vaccinia virus growth factor. Cell 42:383–393
    [Google Scholar]
  28. Twigg A. J., Sherratt D. 1980; Trans-complementable copy-number mutants of plasmid ColEl. Nature; London: 283216–218
    [Google Scholar]
  29. Venkatesan S., Baroudy B. M., Moss B. 1981; Distinctive nucleotide sequences adjacent to multiple initiation and termination sites of an early vaccinia virus gene. Cell 25:805–813
    [Google Scholar]
  30. Wittek R., Menna A., Müller H. K., Schümperli D., Boseley G., Wyler R. 1978; Inverted terminal repeats in rabbit pox virus and vaccinia virus DNA. Journal of Virology 28:171–181
    [Google Scholar]
  31. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequence of M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  32. Yuen L., Moss B. 1987; Oligonucleotide sequence signalling transcriptional termination of vaccinia virus early genes. Proceedings of the National Academy of Sciences, U.S.A 84:6417–6421
    [Google Scholar]
  33. Yuen L., Davison A. J., Moss B. 1987; Early promoter-binding factor from vacciniavirions. Proceedings of the National Academy of Sciences, U.S.A 84:6069–6073
    [Google Scholar]
  34. Zweig M., Helman C. J. JR Rabin H., Hampar B. 1980; Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus. Journal of Virology 35:644–652
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-9-2013
Loading
/content/journal/jgv/10.1099/0022-1317-71-9-2013
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error