RT Journal Article SR Electronic(1) A1 Takahashi, Kazuhiro A1 Kitamura, Naomi A1 Shibui, Tatsurou A1 Kamizono, Michiru A1 Matsui, Rie A1 Yoshiyama, Yoshiko A1 Maeda, Toshiro A1 Kondo, Jun A1 Honda, Yoshikazu A1 Yamada, Ei A1 Shimizu, Yohko K. A1 Teranishi, Yutaka A1 Nakanishi, ShigetadaYR 1990 T1 Cloning, sequencing and expression in Escherichia coli of cDNA for a non-A, non-B hepatitis-associated microtubular aggregates protein JF Journal of General Virology, VO 71 IS 9 SP 2005 OP 2011 DO https://doi.org/10.1099/0022-1317-71-9-2005 PB Microbiology Society, SN 1465-2099, AB A 1·7 kb cDNA encoding a novel antigen (p44; apparent Mr 44K) associated with non-A, non-B (NANB) hepatitis, was isolated from the hepatic cDNA library of a chimpanzee infected with NANB hepatitis. The library was screened with a monoclonal antibody against this antigen. The cDNA cloned contained an open reading frame encoding a 444 amino acid protein with an Mr calculated to be 50468. The cDNA hybridized to a 1·9 kb mRNA obtained from chimpanzee hepatocytes infected with either the NANB or hepatitis delta viruses. It hybridized weakly to mRNA from hepatitis B virus-infected hepatocytes, and not at all to mRNA from normal chimpanzee hepatocytes. Southern blot analysis revealed that p44 is a host protein in chimpanzees, and that an identical gene exists in the human genome., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-9-2005