%0 Journal Article %A Takahashi, Kazuhiro %A Kitamura, Naomi %A Shibui, Tatsurou %A Kamizono, Michiru %A Matsui, Rie %A Yoshiyama, Yoshiko %A Maeda, Toshiro %A Kondo, Jun %A Honda, Yoshikazu %A Yamada, Ei %A Shimizu, Yohko K. %A Teranishi, Yutaka %A Nakanishi, Shigetada %T Cloning, sequencing and expression in Escherichia coli of cDNA for a non-A, non-B hepatitis-associated microtubular aggregates protein %D 1990 %J Journal of General Virology, %V 71 %N 9 %P 2005-2011 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-71-9-2005 %I Microbiology Society, %X A 1·7 kb cDNA encoding a novel antigen (p44; apparent Mr 44K) associated with non-A, non-B (NANB) hepatitis, was isolated from the hepatic cDNA library of a chimpanzee infected with NANB hepatitis. The library was screened with a monoclonal antibody against this antigen. The cDNA cloned contained an open reading frame encoding a 444 amino acid protein with an Mr calculated to be 50468. The cDNA hybridized to a 1·9 kb mRNA obtained from chimpanzee hepatocytes infected with either the NANB or hepatitis delta viruses. It hybridized weakly to mRNA from hepatitis B virus-infected hepatocytes, and not at all to mRNA from normal chimpanzee hepatocytes. Southern blot analysis revealed that p44 is a host protein in chimpanzees, and that an identical gene exists in the human genome. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-9-2005