RT Journal Article SR Electronic(1) A1 Larsen, Charles E. A1 Alford, Dennis R. A1 Young, Lawrence J. T. A1 McGraw, Thomas P. A1 Düzgüneǵ, NejatYR 1990 T1 Fusion of simian immunodeficiency virus with liposomes and erythrocyte ghost membranes: effects of lipid composition, pH and calcium JF Journal of General Virology, VO 71 IS 9 SP 1947 OP 1955 DO https://doi.org/10.1099/0022-1317-71-9-1947 PB Microbiology Society, SN 1465-2099, AB Simian immunodeficiency virus from macaques (SIVmac) is closely related in its structure and biological activity to human immunodeficiency virus, and is the best animal model for the acquired immunodeficiency syndrome. We investigated the kinetics of membrane fusion between SIVmac and phospholipid vesicles and the effects of various parameters on this process. Purified SIVmac was labelled with octadecyl rhodamine B chloride, and fusion was continuously monitored as the dilution of the probe in target membranes. These studies show that SIVmac fusion is strongly dependent upon the liposome composition. Fusion with pure cardiolipin (CL) liposomes is significantly faster than with CL/dioleoylphosphatidylcholine (DOPC) (3:7), phosphatidylserine (PS) or disialoganglioside (GDla)/ DOPC (1·5:8·5) vesicles. SIVmac does not fuse appreciably with pure DOPC liposomes. Reduction of pH from 7·5 to 4·5 greatly enhances the rate of SIVmac fusion with CL, CL/DOPC and PS membranes, but does not affect fusion with DOPC or GDla/DOPC membranes. Calcium stimulates viral fusion with CL liposomes, but not with CL/DOPC or DOPC liposomes. SIVmac fuses with human erythrocyte ghost membranes only slowly at reduced pH. Our results indicate that SIVmac can fuse with membranes lacking the known viral receptor, CD4. Although the mechanism of SIVmac fusion with model and biological membranes remains to be determined, the fusion activity of SIVmac shares similarities with other lipid- enveloped viruses such as Sendai and influenza viruses., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-9-1947