RT Journal Article SR Electronic(1) A1 Tseng, Yi-Hsiung A1 Lo, Mun-Ching A1 Lin, Kuo-Chih A1 Pan, Chao-Chi A1 Chang, Ruey-YiYR 1990 T1 Characterization of Filamentous Bacteriophage 𝜙Lf from Xanthomonas Campestris pv. Campestris JF Journal of General Virology, VO 71 IS 8 SP 1881 OP 1884 DO https://doi.org/10.1099/0022-1317-71-8-1881 PB Microbiology Society, SN 1465-2099, AB A filamentous phage, øLf, which specifically infects Xanthomonas campestris pv. campestris was isolated. The phage particle measured 1000 (±200) × 8 nm. It formed turbid plaques of about 1 mm in diameter. During multiplication, the progeny virions extruded into the medium without retarding host cell growth. Stocks were stable for 6 months at 4 °C and survived treatment at 80 °C for 10 min. Treatment with chloroform, ethanol or acetone completely destroyed infectivity; ethyl ether and methanol inactivated 98 to 99% of the phage. SDS-polyacrylamide gel electrophoresis showed a major coat protein band of approximate Mr 4000 whereas an immunoprecipitation test detected the existence of two coat protein species. The phage genome was shown to be a single-stranded DNA molecule. A physical map was constructed and the DNA size was calculated to be 5·9 kb. Cells treated with Tris-HCl containing CaCl2 and polyethylene glycol 6000 were transfected by replicative form DNA at a frequency of 3·4 × 103 p.f.u./ µg., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-8-1881