%0 Journal Article %A Tseng, Yi-Hsiung %A Lo, Mun-Ching %A Lin, Kuo-Chih %A Pan, Chao-Chi %A Chang, Ruey-Yi %T Characterization of Filamentous Bacteriophage 𝜙Lf from Xanthomonas Campestris pv. Campestris %D 1990 %J Journal of General Virology, %V 71 %N 8 %P 1881-1884 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-71-8-1881 %I Microbiology Society, %X A filamentous phage, øLf, which specifically infects Xanthomonas campestris pv. campestris was isolated. The phage particle measured 1000 (±200) × 8 nm. It formed turbid plaques of about 1 mm in diameter. During multiplication, the progeny virions extruded into the medium without retarding host cell growth. Stocks were stable for 6 months at 4 °C and survived treatment at 80 °C for 10 min. Treatment with chloroform, ethanol or acetone completely destroyed infectivity; ethyl ether and methanol inactivated 98 to 99% of the phage. SDS-polyacrylamide gel electrophoresis showed a major coat protein band of approximate Mr 4000 whereas an immunoprecipitation test detected the existence of two coat protein species. The phage genome was shown to be a single-stranded DNA molecule. A physical map was constructed and the DNA size was calculated to be 5·9 kb. Cells treated with Tris-HCl containing CaCl2 and polyethylene glycol 6000 were transfected by replicative form DNA at a frequency of 3·4 × 103 p.f.u./ µg. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-71-8-1881