1887

Abstract

A filamentous phage, Lf, which specifically infects pv. was isolated. The phage particle measured 1000 (±200) × 8 nm. It formed turbid plaques of about 1 mm in diameter. During multiplication, the progeny virions extruded into the medium without retarding host cell growth. Stocks were stable for 6 months at 4 °C and survived treatment at 80 °C for 10 min. Treatment with chloroform, ethanol or acetone completely destroyed infectivity; ethyl ether and methanol inactivated 98 to 99% of the phage. SDS-polyacrylamide gel electrophoresis showed a major coat protein band of approximate 4000 whereas an immunoprecipitation test detected the existence of two coat protein species. The phage genome was shown to be a single-stranded DNA molecule. A physical map was constructed and the DNA size was calculated to be 5·9 kb. Cells treated with Tris-HCl containing CaCl and polyethylene glycol 6000 were transfected by replicative form DNA at a frequency of 3·4 × 10 p.f.u./ µg.

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1990-08-01
2024-04-16
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References

  1. Atkins D. T., Barber C. E., Daniels M. J. 1987; Transformation of Xanthomonas campestrispathovar campestriswith plasmid DNA. Journal of General Microbiology 133:2727–2731
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Cavalieri S. J., Goldthwait D. A., Neet K. E. 1976; The isolation of a dimer of gene 8 protein of bacteriophage fd. Journal of Molecular Biology 102:712–722
    [Google Scholar]
  4. Dai H., Chiang K.-S., Kuo T.-T. 1980; Characterization of a new filamentous phage Cf from Xanthomonas citri . Journal of General Virology 46:277–289
    [Google Scholar]
  5. Day L. A., Marzec C. J., Reisberg S. A., Casadevall A. 1988; DNA packing in filamentous bacteriophages. Annual Review of Biophysics and Biophysical Chemistry 17:509–539
    [Google Scholar]
  6. Ditta G., Stanfield S., Corbin D., Helinski D. R. 1980; Broad host range DNA cloning system for Gram-negative bacteria: construction of a gene bank of Rhizobium meliloti . Proceedings of the National Academy of Sciences U.S.A.: 777347–7351
    [Google Scholar]
  7. Eisensfork A. 1967; Bacteriophage techniques. IN Methods in Virology 1 pp 449–524 Maramorosch K., Koprowski H. Edited by New York: Academic Press;
    [Google Scholar]
  8. Kuo T. T., Huang T. C., Chow T. Y. 1969; A filamentous bacteriophage from Xanthomonas oryzae . Virology 39:548–555
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 111680–685
    [Google Scholar]
  10. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Messing J. 1983; New M13 vectors for cloning. Methods in Enzymology 101:20–78
    [Google Scholar]
  12. Miller J. H. 1972 Experiments in Molecular Genetics pp 319–325 New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Ouchterlony O., Nilsson A. L. 1978; Immunodiffusion and immunoelectrophoresis. In Handbook of Experimental Immunology pp 19.1–19.14 Weir D. M. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  14. Pai M. S. 1989 Studies on the insertion of filamentous phage DNA into the host chromosome M.S. thesis National Taiwan University:
    [Google Scholar]
  15. Schaller H. 1987; The intergenic region and the origins for filamentous phage DNA replication. Cold Spring Harbor Symposia on Quantitative Biology 43:401–408
    [Google Scholar]
  16. Slodki M. E., Cadmus M. C. 1978; Production of microbial polysaccharides. Advances in Applied Microbiology 23:19–54
    [Google Scholar]
  17. Tseng Y. H., Lo M. C. 1986; A novel filamentous phage of Xanthomonas campestris pr. campestris. Abstracts . Annual Meeting of the American Society for Microbiology M21:236
    [Google Scholar]
  18. Tseng Y. H., Chang R. Y., Fu J. F. 1989; Integration of cloned DNA into chromosome of Xanthomonas campestris mediated by the filamentous phage 𝜙Lf DNA. Abstracts . Annual Meeting of the American Society for Microbiology 310:
    [Google Scholar]
  19. Williams P. H. 1980; Black rot: a continuing threat to world crucifers. Plant Diseases 64:736–742
    [Google Scholar]
  20. Yang B. Y., Tsai H. F., Tseng Y. H. 1987; Molecular cloning of exopolysaccharide synthesis genes of Xanthomonas campestrispv. campestris . In Proceedings of the ROC-USA Agricultural Biotechnology Workshop Taipei, 6to 10 April 1987 pp 229–243 Taipei: National Science Council;
    [Google Scholar]
  21. Yang M.-K., Kuo T.-T. 1984; A physical map of the filamentous bacteriophage Cf genome. Journal of General Virology 65:1173–1181
    [Google Scholar]
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