DNA complementary to the ninth largest (S9) of the 12 genome segments of rice gall dwarf virus (RGDV) was cloned and its sequence was determined. It is 1202 nucleotides in length and contains one open reading frame which extends for 969 nucleotides from nucleotide 26. It encodes a polypeptide of 323 amino acids with an Mr of 35560. The dinucleotide sequence at the 5′ end and the trinucleotide sequence at the 3′ end of the plus strand, 5′ GG—GAU 3′, which are present in the RNA of both wound tumour virus (WTV) and rice dwarf virus (RDV), were also found in RGDV genome segment S9. The nucleotide sequences in the non-coding region at the 5′ terminus and in the 15 nucleotides at the 3′ terminus, which form an imperfect inverted repeat of 10 bp together with the 5′ terminus, are approximately 70% homologous with those of the WTV genome segment S9, but only 30% and 50% homologous with the respective termini of RDV S9.
AnzolaJ. V., XuZ., AsamizuT., NussD. L.1987; Segment-specific inverted repeats found adjacent to conserved terminal sequence in wound tumor virus genome and defective interfering RNAs. Proceedings of the national academy of sciences U.S.A.: 848301–8305
AsamizuT., SummersD., MotikaM. B., AnzolaJ. V., NussD. L.1985; Molecular cloning and characterization of the genome of wound tumor virus: a tumor-inducing plant reovirus. Virology 144:398–409
CashdollarL. W., EsparzaJ., HudsonG. R., ChmeloR., LeeP. W. K., JoklikW. K.1982; Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences U.S.A.: 797644–7648
NeedlemanS. B., WunschC. D.1970; A general method applicable to the search for similarities in the amino acid sequence of two proteins. Journal of Molecular Biology 48:443–453
OmuraT., MinobeY., MatsuokaM., NozuY., TsuchizakiT., SaitoY.1985; Location of structural proteins in particles of rice gall dwarf virus. Journal of General Virology 66:811–815
OmuraT., IshikawaK., HiranoH., UgakiM., MinobeY., TsuchizakiT., KatoH.1989; The outer capsid protein of rice dwarf virus is encoded by genome segment S8. Journal of General Virology 70:2759–2764
UyedaI., MatsumuraT., SanoT., OhshimaK., ShikataE.1987; Nucleotide sequence of rice dwarf virus genome segment 10. Proceedings of the Japan Academy 63:227–230
XuZ., AnzolaJ. V., NalinC. M., NussD. L.1989; The 3′- terminal sequence of a wound tumor virus transcript can influence conformational and functional properties associated with the 5′- terminus. Virology 170:511–522