1887

Abstract

The herpes simplex viruses (HSV-1 and HSV-2) encode a ribonucleotide reductase consisting of two non-identical subunits (RR and RR) which associate to form the active holoenzyme. To facilitate the purification and subsequent biochemical characterization of this enzyme, we have cloned the small subunit 2 of the HSV-2 ribonucleotide reductase (RR ) in a helper-independent adenovirus type 5 vector under the control of the adenovirus type 2 major late promoter. After infection of 293 cells with the recombinant virus, the amount of RR protein produced was eightfold higher than in HSV-2-infected cells. The specific activities of the RR recombinant subunit and the RR protein in HSV-2-infected cells were determined by their mixing with saturating amounts of isolated RR subunit. By comparison of the relative amount of each RR subunit with its specific activity, we calculated that the recombinant protein intrinsic activity was similar to that of the protein produced in HSV-2-infected cells. These results demonstrated that the adenovirus expression vector is a good system to produce an active RR subunit in fairly high amounts.

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1990-08-01
2024-12-13
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