1887

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Volume 71, Issue 8

Virus Neutralizing Activity Induced by Synthetic Peptides of Glycoprotein D of Herpes Simplex Virus Type 1, selected by their Reactivity with Hyperimmune Sera from Mice Free

Abstract

Mice were immunized with synthetic peptides covering the first 56 amino acids of herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) and a fusion protein, produced in , containing the first 55 amino acid residues of gD. It was found that mice immunized with peptides composed of amino acid residues 1 to 13, 18 to 30, 22 to 38 and 38 to 56 of gD were not significantly protected against a lethal challenge with HSV-1. Immunization with peptide 9–21 and the gD fusion protein resulted in significant protection. Antisera, from mice immunized with HSV-1, were investigated for reactivity with a series of 57 overlapping gD peptides covering the entire amino acid sequence, except for the membrane-spanning region. All antisera reacted with peptides 9–21, 10–24, 151–165, 216–232, 282–301 and with peptide 340–354 located in the anchoring region of gD, and 15 other peptides were recognized by at least one antiserum. Twelve peptides (10–24, 151–165, 216–232, 244–267, 260–274, 270–284, 260–284, 282–301, 300–314, 340–354, 348–362 and 355–369) reacted most frequently with the hyperimmune sera from mice and were selected for further study. These were conjugated to bovine serum albumin and used to immunize rabbits. Only antisera against peptide 10–24, which covers the same epitope as peptide 9–21, neutralized HSV-1 .

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1990
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1990-08-01
2024-04-25
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References

  1. Arnon R., Teicher E., Scheraga H. A. 1974; Correlation of conformation and biological activity in lysozyme loop homologs. Journal of Molecular Biology 90:403–407
     [Google Scholar]
  2. Barany G., Merrifield R. B. 1980; Solid-phase peptide synthesis. In The Peptides: Analysis, Synthesis, Biology 2 pp 1–248 Gross E., Meienhofer J. Edited by New York: Academic Press;
     [Google Scholar]
  3. Blumberg B. M., Giorgi C., Roux L., Raju R., Dowling P., Chollet A., Kolakofsky D. 1985; Sequence determination of the Sendai virus HN gene and its comparison to the influenza virus glycoproteins. Cell 41:269–278
     [Google Scholar]
  4. Bosch D. L., Geerligs H. J., Weijer W. J., Feijlbrief M., Welling G. W., Welling-Wester S. 1987; Structural properties and reactivity of N-terminal synthetic peptides of herpes simplex virus type 1 glycoprotein D by using anti-peptide antibodies and group VII monoclonal antibodies. Journal of Virology 61:3607–3611
     [Google Scholar]
  5. Chan W. L. 1983; Protective immunization of mice with specific HSV-1 glycoproteins. Immunology 49:343–352
     [Google Scholar]
  6. Cohen G. H., Dietzschold B., Ponce De Leon M., Long D., Golub E., Varrichio A., Pereira L., Eisenberg R. J. 1984; Localization and synthesis of an antigenic determinant of herpes simplex glycoprotein D that stimulates production of neutralizing antibody. Journal of Virology 49:102–108
     [Google Scholar]
  7. Cohen G. H., Isola V. J., Kuhns J., Berman P. W., Eisenberg R. J. 1986; Localization of discontinuous epitopes of herpes simplex virus glycoprotein D: use of nondenaturing (“native” gel) system of polyacrylamide gel electrophoresis coupled with western blotting. Journal of Virology 60:157–166
     [Google Scholar]
  8. Dietzschold B., Eisenberg R. J., Ponce De Leon M., Golub E., Hudecz F., Varrichio A. 1984; Fine structure analysis of type-specific and type-common antigenic sites of herpes simplex virus glycoprotein D. Journal of Virology 52:431–435
     [Google Scholar]
  9. Eisenberg R. J., Long D., Pereira L., Hampar B., Zweig M., Cohen G. H. 1982; Effect of monoclonal antibody on limited proteolysis of native glycoprotein D of herpes simplex virus type 1. Journal of Virology 41:478–488
     [Google Scholar]
  10. Eisenberg R. J., Long D., Ponce De Leon M., Matthews J. T., Spear P. G., Gibson M. G., Lasky L. A., Berman P., Golub E., Cohen G. H. 1985a; Localization of epitopes of herpes simplex virus type 1 glycoprotein D. Journal of Virology 53:634–644
     [Google Scholar]
  11. Eisenberg R. J., Cerini C. P., Heilman C. J., Joseph A. D., Dietzschold B., Golub E., Long D., Ponce De Leon M., Cohen G. H. 1985b; Synthetic glycoprotein D-related peptides protect mice against herpes simplex virus challenge. Journal of Virology 56:1014–1017
     [Google Scholar]
  12. Fuller O. A., Spear P. G. 1985; Specificities of monoclonal and polyclonal antibodies that inhibit adsorption of herpes simplex virus to cells and lack of inhibition by potent neutralizing antibodies. Journal of Virology 55:475–482
     [Google Scholar]
  13. Geerligs H. J., Weijer W. J., Bloemhoff W., Welling G. W., Welling-Wester S. 1988; The influence of pH and ionic strength on the coating of peptides of herpes simplex virus type 1 in an enzyme-linked immunosorbent assay. Journal of Immunological Methods 106:239–244
     [Google Scholar]
  14. Geerligs H. J., Weijer W. J., Welling G. W., Welling-Wester S. 1989; The influence of different adjuvants on the immune response to a synthetic peptide comprising amino acids residues 9–21 of herpes simplex virus type 1 glycoprotein D. Journal of Immunological Methods 124:95–102
     [Google Scholar]
  15. Hall M. J., Katrak K. 1986; The quest for a herpes simplex virus vaccine: background and recent developments. Vaccine 4:138–150
     [Google Scholar]
  16. Highlander S. L., Sutherland S. L., Gage P. J., Johnson D. C., Levine M., Glorioso J. C. 1987; Neutralizing monoclonal antibodies specific for herpes simplex virus glycoprotein D inhibit virus penetration. Journal of Virology 61:3356–3364
     [Google Scholar]
  17. Houghten A. 1985; General method for the rapid solid phase synthesis of large numbers of peptides: specificity of antigen- antibody interaction at the level of individual amino acids. Proceedings of the National Academy of Sciences U.S.A.: 825131–5135
     [Google Scholar]
  18. Isola V. J., Eisenberg R. J., Siebert G. H., Heilman C. J., Wilcox W. C., Cohen G. H. 1989; Fine mapping of antigenic site II of herpes simplex virus glycoprotein D. Journal of Virology 63:2325–2334
     [Google Scholar]
  19. Jacob C. O., Sela M., Arnon R. 1983; Antibodies against synthetic peptides of the B subunit of cholera toxin. Proceedings of the National Academy of Sciences U.S.A.: 807611–7615
     [Google Scholar]
  20. Johnson D. C., Ligas M. W. 1988; Herpes simplex viruses lacking glycoprotein D are unable to inhibit virus penetration: quantitative evidence for virus-specific cell surface receptors. Journal of Virology 62:4605–4612
     [Google Scholar]
  21. Johnson D. C., Spear P. G. 1989; Herpes simplex virus glycoprotein D mediates interference with herpes simplex virus infection. Journal of Virology 63:819–827
     [Google Scholar]
  22. Kocken C. H. M., Geerligs H. J., Bos C. A., Ab G., Weijer W. J., Drijfhout J. W., Welling G. W., Welling-Wester S. 1988; Immunological properties of an N-terminal fragment of herpes simplex virus type 1 glycoprotein D expressed in Escherichia coli . Archives of Virology 103:267–274
     [Google Scholar]
  23. Lasky L. A., Dowbenko D. 1984; DNA sequence analysis of the type common glycoprotein-D genes of herpes simplex virus type 1 and 2. DNA 3:23–29
     [Google Scholar]
  24. Long D., Madara T. J., Ponce De Leon M., Cohen G. H., Montgomery P. C., Eisenberg R. J. 1984; Glycoprotein D protects mice against lethal challenge with herpes simplex virus type 1 and 2. Infection and Immunity 37:761–764
     [Google Scholar]
  25. Minson A. C., Hodgman T. C., Digard P., Hancock D. C., Bell S. E., Buckmaster E. A. 1986; An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization. Journal of General Virology 67:1001–1013
     [Google Scholar]
  26. Muggeridge M. I., Isola V. J., Byrn R. A., Tucker T. J., Minson A. C., Glorioso J. C., Cohen G. H., Eisenberg R. J. 1988; Antigenic analysis of a major neutralization site of herpes simplex virus glycoprotein D, using deletion mutants and monoclonal antibody-resistant mutants. Journal of Virology 62:3274–3280
     [Google Scholar]
  27. Rawls W. E., Balachandran N., Sisson G., Watson R. J. 1984; Localization of a type specific antigenic site on herpes simplex virus type 2 glycoprotein D. Journal of Virology 51:263–265
     [Google Scholar]
  28. Rector J. T., Lausch R. N., Oakes J. E. 1982; Use of monoclonal antibodies for analysis of antibody-dependent immunity to ocular herpes simplex virus type 1 infection. Infection and Immunity 38:168–174
     [Google Scholar]
  29. Renis H. E., Eidson E. E., Mathews J., Gray E. 1976; Pathogenesis of herpes simplex virus types 1 and 2 in mice after various routes of inoculation. Infection and Immunity 14:571–578
     [Google Scholar]
  30. Strynadka N. C. J., Redmond M. J., Robertparker J. M., Scraba D. G., Hodges R. S. 1988; Use of synthetic peptides to map the antigenic determinants of glycoprotein D of herpes simplex virus. Journal of Virology 62:3474–3483
     [Google Scholar]
  31. Van Der Ploeg J. R., Drijfhout J. W., Feijlbrief M., Bloemhoff W., Welling G. W., Welling-Wester S. 1989; Immunological properties of multiple repeats of a linear epitope of herpes simplex virus type 1 glycoprotein D. Journal of Immunological Methods 124:211–217
     [Google Scholar]
  32. Watari E., Dietzschold B., Szokan G., Heber-Katz E. 1987; A synthetic peptide induces long-term protection from lethal infection with herpes simplex virus type 2. Journal of Experimental Medicine 165:459–470
     [Google Scholar]
  33. Watson R. J. 1983; DNA sequence of the herpes simplex virus type 2 glycoprotein D gene. Gene 26:307–312
     [Google Scholar]
  34. Watson R. J., Weis J. H., Salstrom J. S., Enquist L. W. 1982; Herpes simplex virus type 1 glycoprotein D gene: nucleotide sequence and expression in Escherichia coli . Science 218:381–383
     [Google Scholar]
  35. Weijer W. J., Driifhout J. W., Geerligs H. J., Bloemhoff W., Feijlbrief M., Bos C. A., Hoogerhout P., Kerling K. E. T., Popken-Boer T., Slopsema K., Wilterdink J. B., Welling G. W., Welling-Wester S. 1988; Antibodies against synthetic peptides of herpes simplex virus type 1 glycoprotein D and their capability to neutralize viral infectivity in vitro . Journal of Virology 62:501–510
     [Google Scholar]
  36. Wootton J. C., Taylor J. G., Jackson A. A., Chambers G. K., Fincham J. R. S. 1975; The amino acid sequence of NeurosporaNADP-specific glutamate dehydrogenase. Biochemical Journal 149:739–748
     [Google Scholar]
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Virus Neutralizing Activity Induced by Synthetic Peptides of Glycoprotein D of Herpes Simplex Virus Type 1, selected by their Reactivity with Hyperimmune Sera from Mice
J. Gen. Virol. 71, 1767 (1990); https://doi.org/10.1099/0022-1317-71-8-1767
/content/journal/jgv/10.1099/0022-1317-71-8-1767
/content/journal/jgv/10.1099/0022-1317-71-8-1767
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