1887

Abstract

Tomato black ring virus RNA-1 was translated in a rabbit reticulocyte lysate. The primary translation product of 250K, which corresponds to its whole coding capacity, was synthesized within 45 min and, during further incubation in the translation medium, was proteolytically processed. Essentially, four cleavage products (PI90, PI20, P60 and P50) were detected and located within P250 by pulse-chase and immunoprecipitation experiments. PI90 is an intermediate cleavage product which is further cleaved to form P60 and PI20. PI20, which contains the region that has been assigned to the virus protease and the virus polymerase, was not further cleaved

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1990-08-01
2024-04-19
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References

  1. Bazan J. F., Fletterick R. J. 1989; Comparative analysis of viral cysteine protease structural models. FEBS Letters 249:5–7
    [Google Scholar]
  2. Berna A., Briand J. P., Stussi-Garaud C., Godefroy-Colburn T., Hirth L. 1984; Immunodetection of a non-structural protein of alfalfa mosaic virus (P2) in infected tomato plants. Annates de Virologie de l’Institut Pasteur 135E:285–296
    [Google Scholar]
  3. Brault V., Hibrand L., Candresse T., Le Gall O., Dunez J. 1989; Nucleotide sequence and genetic organization of Hungarian grapevine chrome mosaic nepovirus RNA-2. Nucleic Acids Research 17:7809–7819
    [Google Scholar]
  4. Carrington J. C., Dougherty W. G. 1987; Processing of the tobacco etch virus 49K protease requires autoproteolysis. Virology 160:355–362
    [Google Scholar]
  5. Franssen H., Goldbach R., Van Kammen A. 1984; Translation of bottom component RNA of cowpea mosaic virus in reticulocyte lysate; faithful proteolytic processing of the primary translation product. Virus Research 1:39–49
    [Google Scholar]
  6. Fritsch C., Mayo M. A., Murant A. F. 1978; Translation of the satellite RNA of tomato black ring virus in vitroand in tobacco protoplasts. Journal of General Virology 40:587–593
    [Google Scholar]
  7. Fritsch C., Mayo M. A., Murant A. F. 1980; Translation products of genome and satellite RNAs of tomato black ring virus. Journal of General Virology 46:381–389
    [Google Scholar]
  8. Fritsch C., Koenig I., Murant A. F., Raschké J. H., Mayo M. A. 1984; Comparisons among satellite RNA species from five isolates of tomato black ring virus and one isolate of myrobalan latent ringspot virus. Journal of General Virology 65:289–294
    [Google Scholar]
  9. Goldbach R., Rezelman G. 1983; Orientation of the cleavage map of the 200-kilodalton polypeptide encoded by the bottom- component RNA of cowpea mosaic virus. Journal of Virology 46:614–619
    [Google Scholar]
  10. Greif C., Hemmer O., Fritsch C. 1988; Nucleotide sequence of tomato black ring virus RNA-1. Journal of General Virology 69:1517–1529
    [Google Scholar]
  11. Hemmer O., Demangeat G., Greif C., Fritsch C. 1989; Detection of a proteolytic activity in the micrococcal nuclease used for preparation of messenger-dependent reticulocyte lysates. FEBS Letters 250:357–361
    [Google Scholar]
  12. Jackson R. J. 1986; A detailed kinetic analysis of the in vitrosynthesis and processing of encephalomyocarditis virus products. Virology 149:114–127
    [Google Scholar]
  13. Jackson R. J., Hunt T. 1983; Preparation and use of nuclease- treated rabbit reticulocyte lysates for the translation of eukaryotic mRNA. Methods in Enzymology 96:50–74
    [Google Scholar]
  14. Koenig I., Fritsch C. 1982; A protein linked at the 5' end of satellite and genomic tomato black ring virus RNAs: study of in vitrotranslation after protease treatment. Journal of General Virology 60:343–353
    [Google Scholar]
  15. Le Gall O., Candresse T., Brault V., Dunez J. 1989; Nucleotide sequence of Hungarian grapevine chrome mosaic nepovirus RNA-1. Nucleic Acids Research 17:7795–7807
    [Google Scholar]
  16. Mayo M. A., Barker H., Harrison B. D. 1979; Polyadenylate in the RNA of five nepoviruses. Journal of General Virology 43:603–610
    [Google Scholar]
  17. Mayo M. A., Barker H., Harrison B. D. 1982; Specificity and properties of the genome-linked proteins of nepoviruses. Journal of General Virology 59:149–162
    [Google Scholar]
  18. Meyer M., Hemmer O., Mayo M. A., Fritsch C. 1986; The nucleotide sequence of tomato black ring virus RNA-2. Journal of General Virology 67:1257–1271
    [Google Scholar]
  19. Pelham H. R. B., Jackson R. J. 1976; An efficient mRNA- dependent translation system from reticulocyte lysates. European Journal of Biochemistry 67:247–256
    [Google Scholar]
  20. Serghini M. A., Pinck M., Fuchs M., Walter B., Pinck L. 1989; RNA 2 of grapevine fanleaf virus (strain F13) cloning, sequence analysis and coat protein location. Abstracts of the IVth International Plant Virus Epidemiology Workshop p 144 Montpellier:
    [Google Scholar]
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