1887

Abstract

Four strains of maize streak virus, namely the and sugarcane strains, have been identified from 19 isolates by ELISA using polyclonal antisera cross-absorbed with particles of the maize strain. The results suggest there is an epitope of the maize strain which is not dependent on the capsid being intact and which is common to all the members of the group; other strain-specific epitopes are probably conformation-dependent. A specific epitope (probably internal) occurs on the coat protein of a maize strain isolate, D(R)D, grown in , that is also present on the coat protein of the (previously reported as ) strain. Specific internal epitopes also occur in the coat proteins of sugarcane and strains. The use of indirect ELISA was necessary for accurate serotyping. The serological reactivities of particles of all the members of each type were identical irrespective of the host from which they were extracted. Sap extracts proved to be more reliable sources of antigen than virus preparations, which could vary in their serological reactivity. Nevertheless, serological differentiation index values determined in tests using either type of antigen proved reliable and consistent.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-8-1635
1990-08-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/8/JV0710081635.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-8-1635&mimeType=html&fmt=ahah

References

  1. Barbara D. J., Clark M. F. 1982; A simple indirect ELISA using F(ab’)2 fragments of immunoglobulin. Journal of General Virology 58:315–322
    [Google Scholar]
  2. Bock K. R. 1974; Maize streak virus. CMI/AAB Descriptions of Plant Viruses133
    [Google Scholar]
  3. Bock K. R., Guthrie E. J., Woods R. D. 1974; Purification of maize streak virus and its relationship to viruses associated with streak diseases of sugarcane and Panicum maximum. Annals of Applied Biology 77:289–296
    [Google Scholar]
  4. Campbell D. H., Garvey J. S., Cremer N. E., Sussdorf D. H. 1970 Methods in Immunology, 2nd edn.. pp 224–232 Reading, Massachusetts: W. A. Benjamin;
    [Google Scholar]
  5. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  6. Clark M. F., Barbara D. J. 1987; A method for the quantitative analysis of ELISA data. Journal of Virological Methods 15:213–222
    [Google Scholar]
  7. Damsteegt V. D. 1983; Maize streak virus: 1 Host range and vulnerability of maize germ plasm. Plant Disease 67:734–737
    [Google Scholar]
  8. Dekker E. L., Pinner M. S., Markham P. G., Van Regenmortel M. H. V. 1988; Characterization of maize streak virus isolates from different plant species by polyclonal and monoclonal antibodies. Journal of General Virology 69:983–990
    [Google Scholar]
  9. Dollet M., Accotto G. P., Lisa V., Menissier J., Boccardo G. 1986; A geminivirus, serologically related to maize streak virus, from Digitaria sanguinalisfrom Vanuatu. Journal of General Virology 67:933–937
    [Google Scholar]
  10. Edwards M. L., Cooper J. I. 1985; Plant virus detection using a new form of indirect ELISA. Journal of Virological Methods 11:309–319
    [Google Scholar]
  11. Francki R. I. B., Milne R. G., Hatta T. 1985; Geminivirus group. In Atlas of Plant Viruses I pp 33–46 Boca Raton: CRC Press;
    [Google Scholar]
  12. Harrison B. D. 1985; Advances in geminivirus research. Annual Review of Phytopathology 23:55–82
    [Google Scholar]
  13. Howarth A. J., Vandemark G. J. 1989; Phytogeny of geminiviruses. Journal of General Virology 70:2717–2727
    [Google Scholar]
  14. Jaegle M., Van Regenmortel M. H. V. 1985; Use of ELISA for measuring the extent of serological cross-reactivity between plant viruses. Journal of Virological Methods 11:189–198
    [Google Scholar]
  15. Koenig R. 1978; ELISA in the study of homologous and heterologous reactions of plant viruses. Journal of General Virology 40:309–318
    [Google Scholar]
  16. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  17. Mullineaux P. M., Donson J., Morris-Krsinich B. A. M., Boulton M. I., Davies J. W. 1984; The nucleotide sequence of maize streak virus DNA. EMBO Journal 3:3063–3068
    [Google Scholar]
  18. Pinner M. S., Markham P. G., Markham R. H., Dekker E. L. 1988; Characterization of maize streak virus: description of strains; symptoms. Plant Pathology 37:74–87
    [Google Scholar]
  19. Ricaud C., Fèlix S. 1976; Identification et importance relative des viroses du maïs a L’lle Maurice. Revue Agricole Sucriere de L’lle Maurice 55:163–169
    [Google Scholar]
  20. Ricaud C., Feèlix S. 1978; Strains of streak virus infecting sugarcane. Proceedings of the International Society of Sugarcane Technologists, Brazil 16:449–457
    [Google Scholar]
  21. Roberts I. M., Robinson D. J., Harrison B. D. 1984; Serological relationships and genome homologies among geminiviruses. Journal of General Virology 65:1723–1730
    [Google Scholar]
  22. Rose D. J. W. 1978; Epidemiology of maize streak disease. Annual Review of Entomology 23:259–282
    [Google Scholar]
  23. Rossel H. W., Thottapilly G. 1984; Maize. Virology. Annual Report. International Institute of Tropical AgricultureIbadan, Nigeria; IITA198552–53 En.
    [Google Scholar]
  24. Seth M. L., Singh D. V., R.Chandhuin S. P. 1972; Bajra (pearl millet) streak: a Ieafhopper-borne cereal virus in India. Plant Disease Reporter 56:424–428
    [Google Scholar]
  25. Soto P. E., Buddenhagen I. W., Asnani V. L. 1982; Development of streak virus-resistant maize populations through improved challenge and selection methods. Annals of Applied Biology 100:539–546
    [Google Scholar]
  26. Stanley J. 1985; The molecular biology of geminiviruses. Advances in Virus Research 30:139–177
    [Google Scholar]
  27. Storey H. H., McClean A. P. D. 1930; The transmission of streak disease between maize, sugarcane and wild grasses. Annals of Applied Biology 17:691–714
    [Google Scholar]
  28. Sutual C. L., Gillet J. M., Morrissey S. M., Ramsdell D. C. 1986; Interpreting ELISA data and establishing the positivenegative threshold. Plant Disease 70:722–726
    [Google Scholar]
  29. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences U.S.A.: 764350–4354
    [Google Scholar]
  30. Van Regenmortel M. H. V., Burckard J. 1980; Detection of a wide spectrum of tobacco mosaic virus strains by indirect enzyme- linked immunosorbent assays (ELISA). Virology 106:327–334
    [Google Scholar]
  31. Van Regenmortel M. H. V., Von Wechmar M. B. 1970; A reexamination of the serological relationship between tobacco mosaic virus and cucumber virus 4. Virology 41:330–338
    [Google Scholar]
  32. Webb M. D. 1987; Species recognition in Cicadulinaleafhoppers (Hemiptera: Cicadellidae), vectors of pathogens of Gramineae. Bulletin of Entomological Research 77:683–712
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-8-1635
Loading
/content/journal/jgv/10.1099/0022-1317-71-8-1635
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error