1887

Abstract

The 3′ half of the RNA of the cucurbit potyvirus zucchini yellow mosaic virus (ZYMV) was genetically cloned and the cDNA sequence of a portion of the putative RNA polymerase gene, the complete coat protein gene and the 3′ untranslated region was determined. The coat protein and putative polymerase genes are both part of a continuous open reading frame as is the case for other potyviruses whose genomes are expressed as polyproteins. The Q/S protease cleavage site for the N terminus of the coat protein was deduced by alignment of the coat protein and polymerase genes with other potyviral sequences. The resulting protein has 279 amino acids and a calculated of 31214. The predicted amino acid sequence indicates a ZYMV- unique N-terminal region and potyvirus-characteristic central and C-terminal regions. These data also verify that ZYMV is distinct from the cucurbit potyvirus watermelon mosaic virus 2.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-7-1619
1990-07-01
2024-10-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/7/JV0710071619.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-7-1619&mimeType=html&fmt=ahah

References

  1. Alderz W. C. 1987; Cucurbit potyvirus transmission by alate aphids trapped alive. Journal of Economic Entomology 80:87–92
    [Google Scholar]
  2. Allison R. F., Dougherty W. G., Parks T. D., Willis L., Johnston R. E., Kelly M., Armstrong F. B. 1985a; Biochemical analysis of the capsid gene and capsid protein of tobacco etch virus: N-terminal amino acids are located on the virion’s surface. Virology 147:309–316
    [Google Scholar]
  3. Allison R. F., Sorenson J. C., Kelly M. E., Armstrong F. B., Dougherty W. G. 1985b; Sequence determination of the capsid protein gene and flanking regions of tobacco etch virus: evidence for synthesis and processing of a polyprotein in potyvirus genome expression. Proceedings of the National Academy of Sciences, V.S.A. 82:3969–3972
    [Google Scholar]
  4. Allison R. F., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
    [Google Scholar]
  5. Baulcombe D. 1989; Strategies for virus resistance in plants. Trends in Genetics 5:56–60
    [Google Scholar]
  6. Davis R. F. 1986; Partial characterization of zucchini yellows mosaic virus isolated from squash in Turkey. Plant Disease 70:735–738
    [Google Scholar]
  7. Davis R. F., Mizuki M. K. 1987; Detection of cucurbit viruses in New Jersey. Plant Disease 71:40–44
    [Google Scholar]
  8. Domier L. L., Franklin K. M., Shahabuddin M., Hellmann G. M., Overmeyer J. H., Hiremath S. T., Siaw M. F. E., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  9. Dougherty W. G., Carrington J. C. 1988; Expression and function of poty viral gene products. Annual Review of Phytopathology 26:123–143
    [Google Scholar]
  10. Dougherty W. G., Willis L., Johnston R. E. 1985; Topographic analysis of tobacco etch virus capsid protein epitopes. Virology 144:66–72
    [Google Scholar]
  11. Gough K. H., Azad A. A., Hanna P. J., Shukla D. D. 1987; Nucleotide sequence of the capsid and nuclear inclusion protein genes from the Johnson grass strain of sugarcane mosaic virus RNA. Journal of General Virology 68:297–304
    [Google Scholar]
  12. Grumet R. 1990; Genetically engineered plant virus resistance. HortScience 25:508–513
    [Google Scholar]
  13. Grumet R., Sanford J. C., Johnston S. A. 1987; Pathogen- derived resistance to viral infection using a negative regulatory molecule. Virology 161:561–569
    [Google Scholar]
  14. Gubler U., Hoffman B. J. 1983; A simple and very efficient method for generating cDNA libraries. Gene 25:263–269
    [Google Scholar]
  15. Hellmann G. M., Shaw J. G., Lesnaw J. A., Chu L-Y., Pirone T. P., Rhoads R. E. 1980; Cell-free translation of tobacco vein mottling virus. Virology 106:207–216
    [Google Scholar]
  16. Kamer G., Argos P. 1984; Primary structural comparisons of RNA-dependent polymerases from plant, animal and bacterial viruses. Nucleic Acids Research 12:7269–7281
    [Google Scholar]
  17. Lecoq H., Pitrat M. 1985; Specificity of the helper component- mediated aphid transmission of three potyviruses infecting musk-melon. Phytopathology 75:890–893
    [Google Scholar]
  18. Lisa V., Lecoq H. 1984; Zucchini yellow mosaic virus. CMI/AAB Descriptions of Plant Viruses282
    [Google Scholar]
  19. Lisa V., Boccardo G., D’Agostino G., Dellavalle G., D’Aquilio M. 1981; Characterization of a potyvirus that causes zucchini yellow mosaic. Phytopathology 571:667–672
    [Google Scholar]
  20. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H. W. D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology 70:513–524
    [Google Scholar]
  21. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Purcifull D., Hiebert E., Edwardson J. 1984a; Watermelon mosaic virus 2. CMI/AAB Descriptions of Plant Viruses293
    [Google Scholar]
  23. Purcifull D., Edwardson J., Hiebert E., Gonsalves D. 1984b; Papaya ringspot virus. CMI/AAB Descriptions of Plant Viruses292
    [Google Scholar]
  24. Ravelonandro M., Varveri C., Delbos R., Dunez J. 1988; Nucleotide sequence of the capsid protein gene of plum pox potyvirus. Journal of General Virology 69:1509–1516
    [Google Scholar]
  25. Register J. C., Beachy R. N. 1988; Resistance to TMV in transgenic plants results from interference with an early event in infection. Virology 166:524–532
    [Google Scholar]
  26. Sanger F. 1981; Determination of nucleotide sequences in DNA. Science 214:1205–1210
    [Google Scholar]
  27. Shukla D. D., Ward C. W. 1989; Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Archives of Virology 106:171–200
    [Google Scholar]
  28. Shukla D. D., Inglis A. S., McKern N. M., Gough K. H. 1986; Coat protein of potyviruses. 2. Amino acid sequence of the coat protein of potato virus Y. Virology 152:118–125
    [Google Scholar]
  29. Shukla D. D., McKern N. M., Ward C. W. 1988; Coat protein of potyviruses. 5. Symptomatology, serology, and coat protein sequences of three strains of passionfruit woodiness virus. Archives of Virology 102:221–232
    [Google Scholar]
  30. Van Der Vlugt R., Allefs S., Dehaan P., Goldbach R. 1989; Nucleotide sequence of the 3′-terminal region of potato virus YN RNA. Journal of General Virology 70:229–233
    [Google Scholar]
  31. Vieira J., Messing J. 1987; Production of single stranded plasmid DNA. Methods in Enzymology 153:3–11
    [Google Scholar]
  32. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  33. Yeh S. D., Gonsalves D. 1985; Translation of papaya ringspot virus RNA in vitro: detection of a possible polyprotein that is processed for capsid protein, cylindrical-inclusion protein, and amorphous-inclusion protein. Virology 143:260–271
    [Google Scholar]
  34. Yu M. H., Frenkel M. J., McKern N. M., Shukla D. D., Strike P. M., Ward C. W. 1989; Coat protein of potyviruses. 6. Amino acid sequences suggest watermelon mosaic virus 2 and soybean mosaic virus-N are strains of the same potyvirus. Archives of Virology 105:55–64
    [Google Scholar]
  35. Zaret K. S., Sherman F. 1982; DNA sequence required for efficient transcription termination in yeasts. Cell 28:563–573
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-71-7-1619
Loading
/content/journal/jgv/10.1099/0022-1317-71-7-1619
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error