1887

Abstract

The pathological changes and distribution of virus antigen in mouse brains were studied following intracranial inoculation of 3 week old BALB/c mice with the herpes simplex virus (HSV) type 2 strain HG52 and its deletion variant JH2604. The variant JH2604 failed to produce necrotizing encephalitis compared to the parental HG52. The morphological changes induced in JH2604-infected brains consisted of localized perivascular cuffing by lymphocytes and infiltration by immune cells. Immunohistochemical studies using polyclonal anti-HSV serum showed that JH2604 antigens were localized at the site of inoculation with no evidence of neuronal involvement. Wild-type HSV- infected brains demonstrated a wide distribution of antigens both in neuronal and supporting cells. These data provide evidence that the non-neurovirulent phenotype of JH2604 is due to inability to replicate within neuronal cells of the central nervous system and pinpoints a precise role for the HG52 sequences contained within the 1488 bp subfragment of TR/IR deleted in JH2604.

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/content/journal/jgv/10.1099/0022-1317-71-7-1597
1990-07-01
2021-10-17
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References

  1. Brown S. M., Harland J. 1987; Three mutants of herpes simplex virus type 2: one lacking the genes US10, US11 and US12 and two in which Rs has been extended by 6 kb to 0·91 map units with loss of Us sequences between 0·94 and the US/TRS junction. Journal of General Virology 68:1–18
    [Google Scholar]
  2. Brown S. M., Harland J., Subak-Sharpe J. H. 1984; Isolation of restriction endonuclease site deletion mutants of herpes simplex virus. Journal of General Virology 65:1053–1068
    [Google Scholar]
  3. Finelli P. F. 1975; Herpes simplex virus and human nervous system: current concept and review. Military Medicine 130:765–771
    [Google Scholar]
  4. Harland J., Brown S. M. 1985; Isolation and characterization of deletion mutants of herpes simplex virus type 2 (strain HG52). Journal of General Virology 66:1305–1321
    [Google Scholar]
  5. Harland J., Brown S. M. 1989; A herpes simplex virus type 2 variant in which a deletion across the L-S junction is replaced by single or multiple reiterations of extraneous DNA. Journal of General Virology 70:2121–2137
    [Google Scholar]
  6. Hayashi K., Iwasaki Y., Yanagi K. 1986; Herpes simplex virus type 1 induced hydrocephalus in mice. Journal of Virology 57:942–951
    [Google Scholar]
  7. Kennedy P. G. E., Narayan O., Ghalbi Z., Hopkins J., Gendelman H. E., Clements J. E. 1985; Persistent expression of la antigen and viral genomes in visna-maedi virus induced inflammatory cells: possible role of lentivirus induced interferon. Journal of Experimental Medicine 162:1970–1982
    [Google Scholar]
  8. McFarland D. J., Hotchin J. 1987; Contrasting patterns of virus spread and neuropathology following microinjection of herpes simplex virus into the hippocampus and cerebellum of mice. Journal of Neurological Sciences 79:258–265
    [Google Scholar]
  9. MaClean A. R., Brown S. M. 1987a; A herpes simplex virus type 1 variant which fails to synthesize immediate early polypeptide VmwIE63. Journal of General Virology 68:1339–1350
    [Google Scholar]
  10. MaClean A. R., Brown S. M. 1987b; Deletion and duplication variants around the long repeats of herpes simplex virus type 1 strain 17. Journal of General Virology 68:3019–3031
    [Google Scholar]
  11. Nahmias A. J., Whitley R. J., Visinline A. N., Takei Y., Alford C. A. J. R. Collaborative Ayrivlral Study Group 1982; Herpes simplex virus encephalitis: laboratory evaluations and their diagnostic significance. Journal of Infectious Diseases 145:829–836
    [Google Scholar]
  12. Stevens J. G., Cook M. L. 1971; Latent herpes simplex virus in spinal ganglia of mice. Science 173:843–847
    [Google Scholar]
  13. Taha M. Y., Clements G. B., Brown S. M. 1989a; A variant of herpes simplex virus type 2 strain HG52 with a T5 kb deletion in RL between 0 to 0·02 and 0·81 to 0·83 map units is non-neurovirulent for mice. Journal of General Virology 70:705–716
    [Google Scholar]
  14. Taha M. Y., Clements G. B., Brown S. M. 1989b; The herpes simplex virus type 2 (HG52) variant JH2604 has a 1488 bp deletion which eliminates neurovirulence in mice. Journal of General Virology 70:3073–3078
    [Google Scholar]
  15. Timbury M. C. 1971; Temperature-sensitive mutants of herpes simplex virus type 2. Journal of General Virology 13:373–376
    [Google Scholar]
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