1887

Abstract

Pulmonary A2 strain respiratory syncytial virus infection of BALB/c laboratory mice persisted for up to 7 days after initial infection with peak virus titres being recovered on day 4. Virus antigen within the lungs was found to be restricted essentially to the alveolar regions. Similarly, pulmonary histopathological changes remained confined to the peri-alveolar regions being consistent with mild pneumonia. Infection was found to elicit a pulmonary major histocompatibility complex-restricted cytotoxic T lymphocyte (CTL) response which was first detectable 6 days after infection and optimal 7 to 9 days after infection. This local CTL response was preceded by a rapid transient virus-specific lymphocyte transformation response which was detectable only 3 days after intranasal infection. In addition, infection induced rapid interferon production within the lungs which was accompanied by an equally rapid rise in pulmonary natural killer (NK) cell cytotoxic activity. Enhanced NK cell cytotoxicity could be detected after only 1 day post-infection and continued to rise to maximum levels on day 3. This response like the acute CTL response was found to be restricted to the lower respiratory tract. IgG was the first class of virus-specific immunoglobulin to be detected in the lungs of infected animals after experimental infection. However, IgG was not detected until day 10 post-infection, 5 days after the initial decline of virus shedding. Virus-specific IgA although detectable did not appear in the lung until day 24.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-7-1561
1990-07-01
2022-08-14
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/7/JV0710071561.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-7-1561&mimeType=html&fmt=ahah

References

  1. Alsheikhly A. R., Örvell C., Harfast B., Anderson T., Norrby E. 1983; Sendai virus-induced cell mediated cytotoxicity in vitro: the role of viral glycoproteins in cell mediated cytotoxicity. Scandinavian Journal of Immunology 111:129–138
    [Google Scholar]
  2. Bangham C.R.M., Askonas B. A. 1986; Murine cytotoxic T cells specific to respiratory syncytial virus recognize different antigenic subtypes of the virus. Journal of General Virology 67:623–629
    [Google Scholar]
  3. Cannon M. J., Stott E. J., Taylor G., Askonas B. A. 1987; Clearance of persistent RSV infections in immunodeficient mice following transfer of primed T cells. Immunology 62:133–138
    [Google Scholar]
  4. Charlton D., Blandford G. 1977; Immunoglobulin class specific antibody response in serum, spleen, lungs and bronchioal- veolar washings after primary and secondary Sendai virus infection of germ free mice. Infection and Immunity 17:521–527
    [Google Scholar]
  5. Chin J., Magoffin R. L., Sheaper L. A., Schieble J. H., Lennette E. H. 1969; Field evaluation of a respiratory syncytial virus vaccine and a trivalent influenza virus vaccine in a pediatric population. American Journal of Epidemiology 89:449–463
    [Google Scholar]
  6. Cranage M. P., Gardner P. S. 1980; Systemic cell-mediated and antibody responses in infants with respiratory syncytial virus infections. Journal of Medical Virology 5:161–170
    [Google Scholar]
  7. Dreizen R. S., Vyshnevetskaya L. O., Bagdamyan E. E., Yankevich O. D., Tarasova L. B., Klenova A. V. 1971; Study of experimental RS virus infection in cotton rats. Virologic and immunofluorescent studies. Voprosy virusologii 16:670–676
    [Google Scholar]
  8. Finter N. B. 1969; Dye uptake methods for assessing viral cytopathbgenicity and their application to interferon assays. Journal of General Virology 5:419–427
    [Google Scholar]
  9. Graham B. S., Perkins M. D., Wright P. F., Karzon D. T. 1988; Primary respiratory syncytial virus infection in mice. Journal of Medical Virology 26:153–162
    [Google Scholar]
  10. Hanada N., Morishima T., Nishikana K., Isomura S., Nagai Y. 1986; Interferon-mediated self limiting growth of respiratory syncytial virus in mouse embryo cells. Journal of Medical Virology 20:363–370
    [Google Scholar]
  11. Horfast B., Örvell C., Alsheikhly A.R, Perlman P., Norrby E. 1980; The role of viral glycoproteins in mumps virus dependent lymphocyte mediated cytotoxicity in vitro. Scandinavian Journal of Immunology 11:391–400
    [Google Scholar]
  12. Kapikian A. Z., Mitchell R. H., Chanock R. M., Schevedoff R, Stewart C. E. 1969; An epidemiological study of altered clinical reactivity to respiratory syncytial virus infection in children previously vaccinated with an inactivated RS virus vaccine. American Journal of Epidemiology 89:401–421
    [Google Scholar]
  13. Kim H. W., Bellanti J. A., Arrobio J. O., Mills J., Brandt C. D., Chanock R. M., Parrott R. H. 1969; Respiratory syncytial virus neutralising activity in nasal secretions following natural infection. Proceedings of the Society for Experimental Biology and Medicine 131:658–661
    [Google Scholar]
  14. Kumagi T., Wong D. T., Ogra P. L. 1985; Development of cell- mediated cytotoxicity activity in the respiratory tract after experimental infection with respiratory syncytial virus. Clinical and Experimental Immunology 61:351–359
    [Google Scholar]
  15. Mcintosh K., Mcquillin J., Gardner P. S. 1979; Cell-free and cell-bound antibody in nasal secretions from infants with respiratory syncytial infection. Infection and Immunity 23:276–281
    [Google Scholar]
  16. Mito K., Chiba Y., Suga K., Nakao T. 1984; Cellular immune response to infection with respiratory syncytial virus and influence of breast feeding on the response. Journal of Medical Virology 14:323–332
    [Google Scholar]
  17. Murphy B. R., Prince G. A., Walsh E. E., Kim H. W., Parrott R. H., Hemming V. G., Rodriguez W. J., Chanock R. M. 1986; Disassociation between serum neutralising and glycoprotein antibody responses of infants and children who received inactivated respiratory syncytial virus vaccine. Journal of Clinical Microbiology 24:197–202
    [Google Scholar]
  18. Openshaw P.J.M. 1989; Flow cytometric analysis of pulmonary lymphocytes from mice infected with respiratory syncytial virus. Clinical and Experimental Immunology 75:324–328
    [Google Scholar]
  19. Owens S. L., Osebold J. W., Zee Y. C. 1981; Dynamics of B-lymphocytes in the lungs of mice exposed to aerosolised influenza virus. Infection and Immunology 33:231–238
    [Google Scholar]
  20. Prince G. A., Bennett-Jensen A., Horswood R. L., Camargo E., Chanock R. M. 1978; The pathogenesis of respiratory syncytial virus infection in cotton rats. American Journal of Pathology 93:771–783
    [Google Scholar]
  21. Prince G. A., Hemming V. G., Horswood R. L., Baron P. A., Chanock R. M. 1987; Effectiveness of topically administered neutralising antibody in experimental immunotherapy of respiratory syncytial virus infection in cotton rats. Journal of Virology 61:1851–1854
    [Google Scholar]
  22. Routledge E. G., Willcocks M.M"SAMSON, Morgan L. A., Scott R., Anderson J. J., Toms G. L. 1988; The purification of four respiratory syncytial virus proteins and their evaluation as protective agents against experimental infection in BALB/c mice. Journal of General Virology 69:293–303
    [Google Scholar]
  23. Scott R., Delandazuri M. D., Gardner P. S., Owen J.J.T. 1976; Detection of antibody to respiratory syncytial virus by membrane fluorescence. Clinical and Experimental Immunology 26:78–85
    [Google Scholar]
  24. Scott R., Kaul A., Scott M.CHIBA, Ogra P. L. 1978; Development of in vitro correlates of cell-mediated immunity to respiratory syncytial virus infections in humans. Journal of Infectious Diseases 137:810–817
    [Google Scholar]
  25. Scott R., Scott M., Toms G. L. 1981; Cellular and antibody responses to respiratory syncytial (RS) virus in human colostrum, maternal blood and cord blood. Journal of Medical Virology 8:5566
    [Google Scholar]
  26. Shapira-Nahor O., Zakay-Rones Z. 1985; Local and peripheral cell mediated immune response to influenza virus in mice. Journal of Medical Virology 15:81–91
    [Google Scholar]
  27. Steele R. W., Hensen S. A., Vincent M. M., Fuccillo D. A., Bellantt J. 1973; , A 51Cr microassay technique for cell mediated immunity to viruses. Journal of Immunology 110:1502–1510
    [Google Scholar]
  28. Sun G. S., Wyde P. R., Wilson S. Z., Knight V. 1983a; Correlation of cytotoxic activity in the lungs and recovery of normal and gamma-irradiated cotton rats from respiratory syncytial virus infection. American Review of Respiratory Disease 128:668–672
    [Google Scholar]
  29. Sun G. S., Wyde P. R., Knight V. 1983b; Cell mediated cytotoxic responses in lungs of cotton rats infected with respiratory syncytial virus. American Review of Respiratory Disease 127:460–464
    [Google Scholar]
  30. Taylor G., Stott E. J., Bew M., Fernie B. F., Cote P. J., Collins A. P., Hughes M., Jebbett J. 1984a; Monoclonal antibodies protect against respiratory syncytial virus infection in mice. Immunology 52:137–142
    [Google Scholar]
  31. Taylor G., Stott E. J., Hughes M., Collins A. P. 1984b; Respiratory syncytial virus infection in mice. Infection and Immunity 43:649–655
    [Google Scholar]
  32. Taylor G., Stott E. J., Hayle A. J. 1985; Cytotoxic lymphocytes in the lungs of mice infected with respiratory syncytial virus. Journal of General Virology 66:2533–2538
    [Google Scholar]
  33. Toms G. L., Gardner P. S., Pullan C. R., Scott M., Taylor C. E. 1980; Secretion of respiratory syncytial virus inhibitors and antibody in human milk throughout lactation. Journal of Medical Virology 5:351–360
    [Google Scholar]
  34. Walsh E. E., Schlesinger J. J., Brandriss M. W. 1984; Protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies. Infection and Immunity 43:756–758
    [Google Scholar]
  35. Welliver R. C., Kaul A., Ogra P. L. 1979; Cell mediated immune response to respiratory syncytial virus infection: relationship to the development of reactive airway disease. Journal of Pediatrics 94:370–375
    [Google Scholar]
  36. Welliver R. C., Kaul T. N., Ogra P. L. 1980; The appearance of cell-bound IgE in respiratory tract epithelium after respiratory syncytial virus infection. New England Journal of Medicine 303:1198–1202
    [Google Scholar]
  37. Welliver R. C., Wong T. D., Sun M., Middleton T., Vaughan R. S., Ogra P. L. 1981; The development of respiratory syncytial virus-specific IgE and the release of histamine in nasopharyngeal secretions after infection. New England Journal of Medicine 305:841–846
    [Google Scholar]
  38. Welliver R. C., Kaul T. N., Sun M., Ogra P. L. 1984; Defective regulation of immune responses in respiratory syncytial virus infection. Journal of Immunology 133:1925–1930
    [Google Scholar]
  39. Wyde P. R., Sun C. S., Knight V. 1983; Replication of respiratory syncytial virus in the lungs of immunodeficient mice. Journal of Reticuloendothelial Society 34:125–129
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-7-1561
Loading
/content/journal/jgv/10.1099/0022-1317-71-7-1561
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error