1887

Journal of General Virology header logo

Volume 71, Issue 7

BK virus early RNA transcripts in stably transformed cells: enhanced levels induced by dibutyryl cyclic AMP, forskolin and 12--tetradecanoylphorbol-13-acetate treatment Free

Abstract

The stably BK virus (BKV)-transformed hamster cell line BKT-1B and control BHK-21 cells were treated with dibutyryl cAMP (bucAMP), the adenylate cyclase activator forskolin, and the tumour-promoting agent 12--tetradecanoylphorbol-13-acetate (TPA). Cultures were stimulated for 30 min (short) and for 24 h (long). Northern blot analysis showed that for bucAMP and TPA both short and long stimulation resulted in significant increases in the levels of BKV early transcripts. Short exposure to forskolin resulted in a moderate increase and long exposure in a definite increase. In all cases the increased levels were maintained for at least 24 h after short stimulation was stopped. Experiments including the transcription inhibitor actinomycin D revealed that the enhanced levels of early BKV expression after treatment with the stimuli were due to induced RNA synthesis rather than to stabilization of the RNA. No DNA amplification of the early BKV sequences could be detected in the induced cells. The results are discussed with regard to possible roles for a cAMP-responsive element and a phorbol ester-responsive element, shown by sequencing to be present in the control region of the integrated BKV genome of the BKT-1B cells.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1990
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-7-1461
1990-07-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/7/JV0710071461.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-7-1461&mimeType=html&fmt=ahah

References

  1. Akerblom I. E., Slater E. P., Beato M., Baxter J. D., Mellon P. L. 1988; Negative regulation by glucocorticoids through interference with a cAMP responsive enhancer. Science 2 41:350–353
     [Google Scholar]
  2. Angel P., Imagawa M., Chiu R., Stein B., Imbra R. J., Rahmsdorf H. J., Jonat C., Herrlich P., Karin M. 1987; Phorbol ester-inducible genes contain a common cis element recognized by a TPA-modulated trans-acting factor. Cell 49:729–739
     [Google Scholar]
  3. Angel P., Allegretto E. A., Okino S., Hattori K., Boyle W. J., Hunter T., Karin M. 1988; Oncogene jun encodes a sequence specific trans-activator similar to AP-1. Nature; London: 332166–171
     [Google Scholar]
  4. Ball R. K., Siegl B., Quellhorst S., Brandner G., Braun D. G. 1984; Monoclonal antibodies against simian virus 40 nuclear large T tumour antigen: epitope mapping, papovavirus cross reaction and cell surface staining. EMBO Journal 3:1485–1491
     [Google Scholar]
  5. Barbu V., Dautry F. 1989; Northern blot normalization with a 28S rRNA oligonucleotide probe. Nucleic Acids Research 17:7115
     [Google Scholar]
  6. Blumberg P. M. 1981; In vitro studies on the mode of action of the phorbol ester potent tumor promoters. CRC Critical Review of Toxicology 9:153–197
     [Google Scholar]
  7. Bohmann D., Bos T. J., Admon A., Nishimura T., Vogt P. K., Tjian R. 1988; Human proto-oncogene c-jun encodes a DNA binding protein with structural and functional properties of transcription factor AP-1. Science 238:1386–1392
     [Google Scholar]
  8. Cassill J. A., Deyerle K. L., Subramani S. 1989; Unidirectional deletion and linker scan analysis of the late promoter of the human papovavirus BK. Virology 169:172–181
     [Google Scholar]
  9. Chakraborty T., Das G. C. 1989; Identification of HeLa cell nuclear factors that bind to and activate the early promoter of human polyomavirus BK in vitro . Molecular and Cellular Biology 9:3821–3828
     [Google Scholar]
  10. Church G. M., Gilbert W. 1984; Genomic sequencing. Proceedings of the National Academy of Sciences, U.S.A 81:1991–1995
     [Google Scholar]
  11. Cohen P. 1985; The coordinated control of metabolic pathways by broad-specificity protein kinases and phosphatases. Current Topics in Cellular Regulation 27:23–37
     [Google Scholar]
  12. Comb M., Birnberg N. C., Seasholtz A., Herbert E., Goodman H. W. M. 1986; A cyclic AMP-and phorbol ester-inducible DNA element. Nature; London: 323353–356
     [Google Scholar]
  13. Curran T. 1988; Fos and Jun: the AP-1 connection. Cell 55:395–397
     [Google Scholar]
  14. Deyerle K. L., Subramani S. 1988; Linker scan analysis of the early regulatory region of human papovavirus BK. Journal of Virology 62:3378–3387
     [Google Scholar]
  15. Feinberg A., Vogelstein B. 1983; A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132:6–13
     [Google Scholar]
  16. Flægstad T., Traavik T., Christie K. E., Jørgensen J. 1986; Neutralization test for BK virus: plaque reduction detected by immunoperoxidase. Journal of Medical Virology 19:287–296
     [Google Scholar]
  17. Gardner S., Field A., Coleman D., Humle B. 1971; New human papovavirus (BK) isolated from urine after renal transplantation. Lancet i:1253–1257
     [Google Scholar]
  18. Gyllenstein U. B., Erlich H. A. 1988; Generation of single stranded DNA by polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proceedings of the National Academy of Sciences, U.S.A 85:7652–7656
     [Google Scholar]
  19. Hara K., Oya Y., Kinoshita H., Taguchi F., Yogo Y. 1986; Sequence reiteration required for the efficient growth of BK virus. Journal of General Virology 67:2555–2559
     [Google Scholar]
  20. Hirt B. 1967; Selective extraction of polyomavirus DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
     [Google Scholar]
  21. Hollstein M., Yamasaki H. 1987; Tumor promoter-mediated modulation of cell differentiation and communication: the phorbol ester-oncogene connection. In Tumor Cell Differentiation Biology and Pharmacology pp. 317–339 Aarbakke J., Chang P., Koeffler H. Edited by Clifton, New Jersey: Humana Press;
     [Google Scholar]
  22. Imbra R. J., Karin M. 1986; Phorbol ester induces the transcriptional stimulatory activity of the SV40 enhancer. Nature; London: 329555–558
     [Google Scholar]
  23. Lee W., Mitchell P., Than R. 1987; Purified transcription factor AP-1 interacts with TPA-inducible enhancer element. Cell 49:741–752
     [Google Scholar]
  24. Macdonald R. J., Swift G. H., Przybyla A. E., Chirgwin J. M. 1987; Isolation of RNA using guanidinium salts. Methods in Enzymology 152:219–227
     [Google Scholar]
  25. Mcintyre P., Stark G. R. 1988; A quantitative method for analyzing specific DNA sequences directly from whole cells. Analytical Biochemistry 174:209–214
     [Google Scholar]
  26. Mcknight G. S., Clegg C. H., Uhler M. D., Cfirivia J. C., Cadd G. G., Correll L. A., Otten A. D. 1988; Analysis of the cAMP-dependent protein kinase system using molecular genetic approaches. Recent Progress in Hormone Research 44:307–335
     [Google Scholar]
  27. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  28. Markowitz R. B., Dynan W. S. 1988; Binding of cellular proteins to the regulatory region of human papovavirus BK. Journal of Virology 62:3378–3387
     [Google Scholar]
  29. Montminy M. R., Bilezikjian L. M. 1987; Binding of a nuclear protein to the cAMP responsive element of the somatostatin gene. Nature; London: 328305–312
     [Google Scholar]
  30. Montminy M. R., Sevarino K. A., Wagner J. A., Mandel G., Goodman R. H. 1986; Identification of a cyclic-AMP-responsive element within the rat somatostatin gene. Proceedings of the National Academy of Sciences, U.S.A 83:6682–6686
     [Google Scholar]
  31. Nase L. M., Kärkkäinen M., Mäntyjärvi R. A. 1975; Transplantable hamster tumors induced with the BK virus. Acta pathologica et microbiologica scandinavica 83:347–352
     [Google Scholar]
  32. Nishizuka Y. 1986; Studies and perspectives of protein kinase C. Science 233:305–312
     [Google Scholar]
  33. Rauscher F. J. III Cohen D. R., Curran T., Bos T. J., Vogt P. K., Bohmann D., Than R., Franza B. R. Jr 1988; Fos-associated protein p39 is the product of the jun proto-oncogene. Science 240:1010–1016
     [Google Scholar]
  34. Roesler W. J., Vandenbark G. R., Hanson R. W. 1988; Cyclic AMP and the induction of eukaryotic gene transcription. Journal of Biological Chemistry 263:9063–9066
     [Google Scholar]
  35. Rubinstein R., Harley E., Pare N. 1987; Structure and function of the transcriptional control region of non-passaged BK virus. Journal of Virology 61:1747–1750
     [Google Scholar]
  36. Sassone-Corsi P., Lamph W. W., Kamps M., Verma I. 1988a; Fos-associated cellular protein p39 is related to nuclear transcription factor AP-1. Cell 54:553–560
     [Google Scholar]
  37. Sassone-Corsi P., Visvader J., Ferland L., Mellon P. L., Verma I. 1988b; Induction of proto-oncogene fos transcription through the adenylatecyclase pathway: characterization of a cAMP-responsive element. Genes and Development 2:1529–1538
     [Google Scholar]
  38. Seamon K. B., Daly J. W. 1986; Forskolin: its biological and chemical properties. Advances in Cyclic and Nucleotide Protein Phosphorylation Research 20:1–150
     [Google Scholar]
  39. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
     [Google Scholar]
  40. Sten M., Tolonen A., Pitko V., Nevalainen T., Mantyjarvi R. 1976; Characterization of cell lines derived from hamster tumors induced with the BK virus. Archives of Virology 50:73–82
     [Google Scholar]
  41. Sugimoto C., Hara K., Taguchi F., Yogo Y. 1989; Growth efficiency of naturally occurring BK virus variants in vivo and in vitro . Journal of Virology 63:3195–3199
     [Google Scholar]
  42. Taylor S. S. 1989; cAMP-dependent protein kinase. Journal of Biological Chemistry 264:8443–8446
     [Google Scholar]
  43. Watanabe S., Yoshiike K. 1989; Inefficient expression in rat cells of transforming gene of BK virus with 68-bp tandem repeats in the viral promoter-enhancers. Virology 169:204–212
     [Google Scholar]
  44. Wasylyk C., Imler J. L., Perz-Mutul J., Wasylyk B. 1987; The c-Ha-ras oncogene and a tumor promoter activate the polyoma virus enhancer. Cell 48:525–534
     [Google Scholar]
  45. Yamaguchi Y., Satake M., Ito Y. 1989; Two overlapping sequence motifs within the polyomavirus enhancer are independently the targets of stimulation by both the tumor promoter 12-O-tetradecanoylphorbol-13-acetate and the Ha-ras oncogene. Journal of Virology 63:1040–1048
     [Google Scholar]
  46. Yamamoto K. K., Gonzales G. A., Biggs W. H. III Montminy M. R. 1988; Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB. Nature; London: 334494–498
     [Google Scholar]
  47. Yang R. C. A., Wu R. 1979; BK virus DNA: complete nucleotide sequence of a human tumor virus. Science 206:456–462
     [Google Scholar]
  48. Yoshiike K., Takemoto K. K. 1986; Studies with BK virus and monkey lymphotropic papovavirus. In The Papovaviridae 1 The Polyomaviruses pp. 295–326 Salzman N. P. Edited by New York & London: Plenum Press;
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-7-1461
Loading
BK virus early RNA transcripts in stably transformed cells: enhanced levels induced by dibutyryl cyclic AMP, forskolin and 12-O-tetradecanoylphorbol-13-acetate treatment
J. Gen. Virol. 71, 1461 (1990); https://doi.org/10.1099/0022-1317-71-7-1461
/content/journal/jgv/10.1099/0022-1317-71-7-1461
/content/journal/jgv/10.1099/0022-1317-71-7-1461
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error