1887

Abstract

The 3′-terminal genomic regions of an isolate of watermelon mosaic virus II (WMVII) and a Florida isolate of zucchini yellow mosaic virus (ZYMV-F) have been cloned. The nucleotide sequence of the WMVII cDNA clone shows the presence of the large nuclear inclusion protein gene, the coat protein gene and 3′ untranslated region. The nucleotide sequence of a ZYMV-F cDNA clone shows the presence of the coat protein gene and 3′ untranslated region. Comparisons of the nucleotide and deduced amino acid sequences of these clones with those from other potyviruses show that WMVII and the soybean mosaic virus N strain are closely related, thus supporting their classification as different strains of the same virus. Our comparisons also indicate that ZYMV-F is a distinct potyvirus type and that its closest relative is WMVII. Phylogenetic analysis using the most-parsimonious branching arrangement derived from the alignment of coat protein gene sequences suggests the existence of two major potyvirus groupings.

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1990-07-01
2022-10-03
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References

  1. Alderz W. C., Purcifull D. E., Simone G. W., Hiebert E. 1983; Zucchini yellow mosaic virus: a pathogen of squash and other cucurbits in Florida. Proceedings of the Florida State Horticultural Society 96:72–74
    [Google Scholar]
  2. Allison R., Johnston R. E., Dougherty W. G. 1986; The nucleotide sequence of the coding region of tobacco etch virus genomic RNA: evidence for the synthesis of a single polyprotein. Virology 154:9–20
    [Google Scholar]
  3. Carrington J. C., Dougherty W. G. 1987a; Small nuclear inclusion protein encoded by a plant potyvirus genome is a protease. Journal of Virology 61:2540–2548
    [Google Scholar]
  4. Carrington J. C., Dougherty W. G. 1987b; Processing of the tobacco etch virus 49K protease requires autoproteolysis. Virology 160:355–362
    [Google Scholar]
  5. Carrington J. C., Dougherty W. G. 1988; A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proceedings of the National Academy of Sciences, U.S.A 85:3391–3395
    [Google Scholar]
  6. Carrington J. C., Cary S. M., Dougherty W. G. 1988; Mutational analysis of tobacco etch virus polyprotein processing: cis and trans proteolytic activities of polyproteins containing the 49- kilodalton proteinase. Journal of Virology 62:2313–2320
    [Google Scholar]
  7. Chala V. H., Harrison C. W., Halliwell R. S. 1987; Identification of two distinct strains of watermelon mosaic virus 2 affecting cucurbits in Texas. Plant Disease 71:750–752
    [Google Scholar]
  8. Davis R. F. 1986; Partial characterization of zucchini yellow mosaic virus isolated from squash in Turkey. Plant Disease 70:735–738
    [Google Scholar]
  9. Davis R. F., Mizuki M. K. 1987; Detection of cucurbit viruses in New Jersey. Plant Disease 71:40–44
    [Google Scholar]
  10. Dayhoff M. O., Schwarz R. M., Orcutt B. C. 1979; A model of evolutionary change in proteins. In Atlas of Protein Sequence and Structure 5 supplement 3 pp. 345–352 Dayhoff M. O. Edited by Washington, D.C: National Biomedical Research Foundation;
    [Google Scholar]
  11. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  12. Domier L. L., Franklin K. M., Shahabuddin M., Hellmann G. M., Overmeyer J. H., Hiremath S. T., Siaw M. F. E., Lomonossoff G. P., Shaw J. G., Rhoads R. E. 1986; The nucleotide sequence of tobacco vein mottling virus RNA. Nucleic Acids Research 14:5417–5430
    [Google Scholar]
  13. Dougherty W. G., Carrington J. C. 1988; Expression and function of potyviral gene products. Annual Review of Phytopathology 26:123–143
    [Google Scholar]
  14. Dougherty W. G., Allison R. F., Parks T. D., Johnston R. E., Feild M. J., Armstrong F. B. 1985; Nucleotide sequence at the 3′ terminus of pepper mottle virus genomic RNA: evidence for an alternative mode of potyvirus capsid protein gene organization. Virology 146:282–291
    [Google Scholar]
  15. Dougherty W. G., Carrington J. C., Cary S. M., Parks T. D. 1988; Biochemical and mutational analysis of a plant virus polyprotein cleavage site. EMBO Journal 7:1281–1287
    [Google Scholar]
  16. Dougherty W. G., Cary S. M., Parks T. D. 1989a; Molecular genetic analysis of a plant virus polyprotein cleavage site: a model. Virology 171:356–364
    [Google Scholar]
  17. Dougherty W. G., Parks T. D., Cary S. M., Bazan J. F., Fletterick R. J. 1989b; Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase. Virology 172:302–310
    [Google Scholar]
  18. Eggenberger A. L., Stark D. M., Beachy R. N. 1989; The nucleotide sequence of a soybean mosaic virus coat protein-coding region and its expression in Escherichia coli, Agrobacterium tumefaciens and tobacco callus. Journal of General Virology 70:1853–1860
    [Google Scholar]
  19. Frenkel M. J., Ward C. W., Shukla D. D. 1989; The use of 3′ non-coding nucleotide sequences in the taxonomy of potyviruses: application to watermelon mosaic virus 2 and soybean mosaic virus-N. Journal of General Virology 70:2775–2783
    [Google Scholar]
  20. Gough K. H., Azad A. A., Hanna P. J., Shukla D. D. 1987; Nucleotide sequence of the capsid and nuclear inclusion protein genes from the Johnson grass strain of sugarcane mosaic virus RNA. Journal of General Virology 68:297–304
    [Google Scholar]
  21. Hammond J., Hammond R. W. 1989; Molecular cloning, sequencing and expression in Escherichia coli of the bean yellow mosaic virus coat protein gene. Journal of General Virology 70:1961–1974
    [Google Scholar]
  22. Higgins D. G., Sharp P. M. 1988; CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
    [Google Scholar]
  23. Higgins D. G., Sharp P. M. 1989; Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS Communications 5:151–153
    [Google Scholar]
  24. Maiss E., Timpe U., Brisske A., Jelkmann W., Casper R., Himmler G., Mattanovich D., Katinger H. W. D. 1989; The complete nucleotide sequence of plum pox virus RNA. Journal of General Virology 70:513–524
    [Google Scholar]
  25. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  27. Miyamoto M. M., Slightom J. L., Goodman M. 1987; Phylogenetic relations of humans and African apes from DNA sequences in the ψn-globin region. Science 238:369–373
    [Google Scholar]
  28. Miyamoto M. M., Koop B. F., Slightom J. L., Goodman M., Tennant M. R. 1988; Molecular systematics of higher primates: genealogical relations and classification. Proceedings of the National Academy of Sciences, U.S.A 85:7627–7631
    [Google Scholar]
  29. Nagel J., Hiebert E. 1985; Complementary DNA cloning and expression of the papaya ringspotpotyvirus sequences encoding capsid protein and a nuclear inclusion-like protein in Escherichia coli. Virology 143:435–441
    [Google Scholar]
  30. Nameth S. T., Dodds J. A., Paulus A. O., Kishaba A. 1985; Zucchini yellow mosaic virus associated with severe diseases of melon and watermelon in Southern California desert valleys. Plant Disease 69:785–788
    [Google Scholar]
  31. Provvidenti R., Gonsalves D., Humaydan H. S. 1984; Occurrence of zucchini yellow mosaic virus in cucurbits from Connecticut, New York, Florida, and California. Plant Disease 68:443–446
    [Google Scholar]
  32. Purcifull D. E., Hiebert E. 1979; Serological distinction of watermelon mosaic virus isolates. Phytopathology 69:112–116
    [Google Scholar]
  33. Purcifull D. E., Alderz W. C., Simone G. W., Hiebert E., Christie S. R. 1984; Serological relationships and partial characterization of zucchini yellow mosaic virus isolated from squash in Florida. Plant Disease 68:230–233
    [Google Scholar]
  34. Quemada H., Kearney C., Gonsalves D., Slightom J. L. 1989; Nucleotide sequences of the coat protein genes and flanking regions of cucumber mosaic virus strains C and WL RNA 3. Journal of General Virology 70:1065–1073
    [Google Scholar]
  35. Quemada H., L’Hostis B., Gonsalves D., Reardon I. M., Heinrikson R., Hiebert E. L., Sieu L. C., Slightom J. L. 1990; The nucleotide sequences of the 3′-terminal regions of papaya ringspot virus strains W and P. Journal of General Virology 71:203–210
    [Google Scholar]
  36. Robaglia C., Durand-Tardif M., Tronchet M., Boudazin G., Astier-Manifacier S., Casse-Delbart F. 1989; Nucleotide sequence of potato virus Y (N strain) genomic RNA. Journal of General Virology 70:935–947
    [Google Scholar]
  37. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences U.S.A 74:5463–5467
    [Google Scholar]
  38. Shukla D. D., Ward C. W. 1988; Amino acid sequence homology of coat proteins as a basis of identification and classification of the potyvirus group. Journal of General Virology 69:2703–2710
    [Google Scholar]
  39. Shukla D. D., Ward C. W. 1989a; Structure of potyvirus coat proteins and its application in the taxonomy of the potyvirus group. Advances in Virus Research 36:273–314
    [Google Scholar]
  40. Shukla D. D., Ward C. W. 1989b; Identification and classification of potyviruses on the basis of coat protein sequence data and serology. Archives of Virology 106:171–200
    [Google Scholar]
  41. Shukla D. D., Mckern N. M., Ward C. W. 1988a; Coat protein of potyviruses. 5. Symptomatology, serology, and coat protein sequences of three strains of passionfruit woodiness virus. Archives of Virology 102:221–232
    [Google Scholar]
  42. Shukla D. D., Strike P. M., Tracy S. L., Gough K. H., Ward C. W. 1988b; The N and C termini of the coat proteins of potyviruses are surface-located and the N terminus contains the major virus-specific epitopes. Journal of General Virology 69:1497–1508
    [Google Scholar]
  43. Slightom J. L., Drong R. F. 1988; Procedures for constructing genomic clone banks. In Plant Molecular Biology Manual pp. A8:1–42 Gelvin S. B., Schilperoort R. A. Edited by Dordrecht: Kluwer Academic Publishers;
    [Google Scholar]
  44. Swofford D. L. 1985 PAUP: Phylogenetic Analysis Using Parsimony Urbana: Illinois Natural History Survey;
    [Google Scholar]
  45. Yeh S.-D., Gonsalves D. 1985; Translation of papaya ringspot virus RNA in vitro: detection of a posiblepolyprotein that is processed for capsid protein, cylindrical-inclusion protein and amorphous-inclusion protein. Virology 143:260–271
    [Google Scholar]
  46. Yu M. H., Frenkel M. J., Mckern N. M., Shukla D. D., Strike P. M., Ward C. W. 1989; Coat protein of potyviruses.6. Amino acid sequences suggest watermelon mosaic virus 2 and soybean mosaic virus-N are strains of the same potyvirus. Archives of Virology 105:55–64
    [Google Scholar]
  47. Zagursky R. J., Baumeister K., Lomax N., Berman M. L. 1985; Rapid and easy sequencing of large double-stranded DNA and supercoiled plasmid DNA. Gene Analytical Techniques 2:89–94
    [Google Scholar]
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