The complete nucleotide sequence of segment 4 of the rice stripe virus (RSV) genome was determined from overlapping cDNA clones and by direct RNA sequencing. The segment has two long open reading frames (ORFs). One of the ORFs (534 bases) is in the 5′ region of the viral sequence, and the other (858 bases) is in the viral complementary sequence of the viral 3′ region. Such ambisense genome organizations have been seen previously in RNAs of phleboviruses, uukuviruses and arenaviruses. The ORF in the viral sequence encodes the major non-structural protein, but the product of the ORF in the viral complementary sequence (Mr 32407) has not yet been identified. The first 18 bases of each of the 5′ and 3′ ends of segment 4 are complementary in sequence. Among the 3′-terminal 16 bases, 14 were identical between RSV genome segments 3 and 4. These terminal sequences are very similar to those of RNAs of phleboviruses and uukuviruses. These and other characteristics indicate a possible evolutionary relationship between RSV and phleboviruses and/or uukuviruses.
AkashiH.,
BishopD. H. L.1983; Comparison of the sequence and coding of La Crosse and snowshoe hare bunyavirus S RNA species. Journal of Virology 45:1155–1158
AuperinD. D.,
RomanowskyV.,
GalinskyM.,
BishopD. H. L.1984; Sequencing studies of Pichindearenavirus S RNA indicate a novel coding strategy, an ambisense viral S RNA. Journal of Virology 52:897–904
CashdollarL. W.,
EsparzaJ.,
HudsonG. R.,
ChmeloR.,
LeeP. W. K.,
JoklikW. K.1982; Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, U.S.A 79:7644–7648
Clerx-van HaasterC. M.,
ClerxJ. P. M.,
UshijimaH.,
AkashiH.,
FullerF.,
BishopD. H. L.1982; The 3′ terminal RNA sequences of bunyaviruses and nairoviruses (Bunyaviridae): evidence of end sequence generic differences within the virus family. Journal of General Virology 61:289–292
CollettM. S.,
PurchioA. F.,
KeeganK.,
FrazierS.,
HaysW.,
AndersonD. K.,
ParkerM. D.,
SchmaljohnC.,
SchmidtJ.,
DalrympleJ. M.1985; Complete nucleotide sequence of the M segment of Rift Valley fever virus. Virology 144:228–245
FalkB. W.,
TsaiJ. H.,
LommelS. A.1987; Differences in level of detection for the maize stripe virus capsid and major non-capsid proteins in plant and insect hosts. Journal of General Virology 68:1801–1811
HayanoY.,
KakutaniT.,
HayashiT.,
MinobeY.1990; Coding strategy of rice stripe virus: major nonstructural protein is encoded in viral RNA segment 4 and coat protein in RNA complementary to segment 3. Virology 176: in press
IshikawaK.,
OmuraT.,
TsuchizakiT.1989; Association of double- and single-stranded RNAs with each of the four components of rice stripe virus. Annals of the Phytopathological Society of Japan 55:315–323
KisoA.,
YamamotoT.1973; Infection and symptom development in rice stripe disease, with special reference to disease-specific protein other than virus. Review of Plant Protection Research 6:75–100
RobesonG.,
El SaidL. H.,
BrandtW.,
DalrympleJ. M.,
BishopD. H. L.1979; Biochemical studies on the phlebotomus fever group viruses (Bunyaviridae family). Journal of Virology 30:339–350
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of National Academy of Sciences, U.S.A 74:5463–5467
SchmaljohnC. S.,
HastyS. E.,
DalrympleJ. M.,
LeducJ. W.,
LeeH. W.,
Von BonsdorffC. H.,
Brummer-KorvenkontioM.,
VaheriA.,
TsaiT. F.,
RegneryH. L.,
GoldgaberD.,
LeeP. W.1985; Antigenic and genetic properties of virus linked to hemorrhagic fever with renal syndrome. Science 227:1041–1044
SimonsJ. F.,
HellmanU.,
PetterssonR. F.1990; Uukuniemi virus S segment: ambisense coding strategy, packaging of complementary strands into virions, and homology to members of the genus phlebovirus. Journal of Virology 64:247–255
ToriyamaS.1986b; An RNA-dependent RNA polymerase associated with the filamentous nucleoproteins of rice stripe virus. Journal of General Virology 67:1247–1255
ToriyamaS.,
WatanabeY.1989; Characterization of single- and double-stranded RNAs in particles of rice stripe virus. Journal of General Virology 70:505–511