1887

Abstract

A subgenomic restriction fragment from cDNA prepared from Coxsackie B2 virus (CVB2) RNA was subeloned into a riboprobe vector allowing the production of enteroviral group-specific RNA probes complementary to either the positive (genomic) or negative (template) strand of enteroviral RNA. These ribo- probes were used to follow productive infection of cultured cells by CVB2; as expected, positive strand RNA was synthesized in approximately 100-fold excess over negative strand. RNA was extracted from muscle biopsy samples from patients with chronic fatigue syndrome and probed for the presence of enteroviral RNA. In cases where enteroviral RNA was detected the amounts of positive and negative strands of enteroviral RNA were approximately equal, in contrast to the situation in lytic infection of cultured cells. This suggests that entrovirus pesistence in muscle is due to a defect in control of viral RNA synthesis.

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1990-06-01
2023-02-06
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References

  1. Archard L. C., Bowles N. E., Behan P. O., Bell E. J., Doyle D. J. 1988; Postviral fatigue syndrome: persistence of enterovirus RNA in muscle and elevated creatine kinase. Journal of the Royal Society of Medicine 81:326–329
    [Google Scholar]
  2. Berger S. L. 1987; Isolation of cytoplasmic RNA. In Guide to Molecular Cloning Techniques pp. 227–234 Berger S. L., Kimmel A. R. Edited by London: Academic Press;
    [Google Scholar]
  3. Bowles N. E., Richardson P. J., Olsen E. G., Archard L. C. 1986; Detection of Coxsackie-B-virus-specific RNA sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy. Lancet i:1120–1123
    [Google Scholar]
  4. Bowles N. E., Sewry C. A., Dubowitz V., Archard L. C. 1987; Dermatomyositis, polymyositis, and Coxsackie-B-virus infection. Lancet i:1004–1007
    [Google Scholar]
  5. Bowles N. E., Rose M. L., Taylor P., Banner N. R., Morgan-Capner P., Cunningham L., Archard L. C., Yacoub M. H. 1989; End-stage dilated cardiomyopathy: persistence of enterovirus RNA in myocardium at cardiac transplantation and lack of immune response. Circulation 80:1128–1136
    [Google Scholar]
  6. Christensen M. L., Pachman L. M., Schneiderman R., Patel D. C., Friedman J. M. 1986; Prevalence of Coxsackie B virus antibodies in patients with juvenile dermatomyositis. Arthritis and Rheumatism 29:1365–1370
    [Google Scholar]
  7. Croen K. D., Ostrove J. M., Dragovic L. J., Smialek J. E., Straus S. E. 1987; Latent herpes simplex virus in human trigeminal ganglia. New England Journal of Medicine 317:1427–1432
    [Google Scholar]
  8. Holmes G. P., Kaplan J. E., Gantz N. M., Komaroff A. L., Schonberger L. B., Straus S. E., Jones J. F., Dubois R. E., Cunningham-Rundles C., Pahwa S., Tosato G., Zegans L. S., Purtilo D. T., Brown N., Schooley R. T., Brus I. 1988; Chronic fatigue syndrome: a working case definition. Annals of Internal Medicine 108:387–389
    [Google Scholar]
  9. Kaczmarek L., Calbretta B., Baserga R. 1985; Expression of cell cycle-dependent genes in phytohemagglutinin stimulated human lymphocytes. Proceedings of the National Academy of Sciences, U.S.A 82:5372–5379
    [Google Scholar]
  10. Melnick J. L. 1982; Enteroviruses. In Viral Infections of Humans 2 pp. 187–251 Evans A. S. Edited by New York: Plenum Press;
    [Google Scholar]
  11. Morgan-Capner P., Richardson P. J., Mcsorley C., Daly K., Pattison J. R. 1984; Virus investigations in heart muscle disease. In Viral Heart Disease pp. 95–115 Bolte H. D. Edited by Berlin: Springer-Verlag;
    [Google Scholar]
  12. Perez-Bercoff R. 1978; Replication of viral RNA. In The Molecular Biology of Picornaviruses pp. 293–318 Perez-Bercoff R. Edited by New York: Plenum Press;
    [Google Scholar]
  13. Rotbart H. A., Abzug M. J., Murray R. S., Murphy N. L., Levin M. J. 1988; Intracellular detection of sense and antisense enteroviral RNA by in situ hybridization. Journal of Virological Methods 22:295–301
    [Google Scholar]
  14. Stalhandske P. O. K., Lindberg M., Pettersson U. 1984; Replicase gene of coxsackievirus B3. Journal of Virology 51:742–746
    [Google Scholar]
  15. Travers R. L., Hughes G. R. V., Cambridge G., Sewell J. R. 1977; Coxsackie B neutralization titres in polymyositis/dermatomyositis. Lancet i:1268
    [Google Scholar]
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