A panel of 10 monoclonal antibodies produced after immunization with two porcine subgroup I rotavirus strains (OSU and A46), and directed against the major inner capsid protein (VP6), fell into six patterns of reactivity when tested against a collection of human and animal group A rotavirus strains. Monoclonal antibodies of pattern 1 recognized all rotavirus strains. Antibodies of patterns 2 and 3 recognized all subgroup II strains and some, but not all, subgroup I strains. Pattern 4 antibodies identified all subgroup I strains and two strains (H2, equine; CC117, porcine) not reactive with reference subgroup monoclonal antibodies (strains non-I non-II). Pattern 5 antibody exhibited the same reactivity as pattern 4 except for not recognizing the non-I non-II equine strain. Pattern 6 antibodies reacted exclusively with subgroup I and non-I non-II rotaviruses of porcine origin. By competitive binding assays, monoclonal antibodies of patterns 4, 5 and 6 appeared to recognize a single antigenic site, which included at least three overlapping epitopes. In immunoblots all monoclonal antibodies, except one, recognized only the trimeric, but not the monomeric form of VP6.
BohlE. H., TheilK. W., SaifL.1984; Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. Journal of Clinical Microbiology 19:312–319
GorzigliaM., LarreaC., LiprandiF., EsparzaJ.1985; Biochemical evidence for the oligomeric (possibly trimeric) structure of the major inner capsid polypeptide (45K) of rotaviruses. Journal of General Virology 66:1889–1900
GorzigliaM., HoshinoY., NishikawaK., MaloyW. L., JonesR. W., KapikianA. Z., ChanockR. M.1988; Comparative sequence analysis of the genomic segment 6 of four rotaviruses each with a different subgroup specificity. Journal of General Virology 69:1659–1669
GreenbergH., McauliffeV., ValdesusoJ., WyattR., FloresJ., KalicaA., HoshinoY., SinghN.1983; Serological analysis of the subgroup protein of rotavirus using monoclonal antibodies. Infection and Immunity 39:91–99
HoshinoY., GorzigliaM., ValdesusoJ., AskaaJ., GlassR., KapikianA. Z.1987; An equine rotavirus (FI-14 strain) which bears both subgroup I and II specificities on its VP6. Virology 157:488–496
KapikianA. Z., ClineW. L., GreenbergH. B., WyattR. G., KalicaA. R., BanksC. E., JamesH. D., FloresJ., ChanockR. M.1981; Antigenic characterization of human and animal rotaviruses by immune adherence hemagglutination assay (IAHA): evidence for distinctness of IAHA and neutralization antigens. Infection and Immunity 33:415–425
LiprandiF., BritoB., PalenciaL., EsparzaJ.1986; Derivation of a monoclonal antibody against the group specific antigen of rotaviruses and its use in a diagnostic enzymatic immunoassay. Actacientificavenezolana 37:432–436
LiprandiF., GarciaD., BoteroL., GorzigliaM., CavazzaM. E., Perez-SchaelI., EsparzaJ.1987; Characterization of rotaviruses isolated from pigs with diarrhea in Venezuela. Veterinary Microbiology 13:35–45
MattionN. M., BellinzoniR. C., BlackhallJ. O., La TorreJ. L., ScodellerE. A.1989; Antigenic characterization of swine rotaviruses in Argentina. Journal of Clinical Microbiology 26:795–798
SabaraM., ReadyK. F. M., FrenchickP. J., BabiukL. A.1987; Biochemical evidence for the oligomeric arrangement of bovine rotavirus nucleocapsid protein and its possible significance in the immunogenicity of this protein. Journal of General Virology 68:123–133
SvenssonL., GrahnquistL., PettersonC., GrandienM., StintzingG., GreenbergH. B.1988; Detection of human rotaviruses which do not react with subgroup I- and II-specific monoclonal antibodies. Journal of Clinical Microbiology 26:1238–1240