Murine herpesvirus 68 is genetically related to the gammaherpesviruses Epstein-Barr virus and herpesvirus saimiri Free

Abstract

Short nucleotide sequence analysis of seven restriction fragments of murine herpesvirus 68 (MHV-68) DNA has been undertaken and used to determine the overall genome organization and relatedness of this virus to other well characterized representatives of the alpha-, beta- and gammaherpesvirus subgroups. Nine genes have been identified which encode amino acid sequences with greater similarity to proteins of the gammaherpesvirus Epstein-Barr virus (EBV) than to the homologous products of the alphaherpesviruses varicella-zoster virus and herpes simples virus type 1 or the betaherpesvirus human cytomegalovirus. In addition, the genome organization of MHV-68 is shown to have an overall collinearity with that of the gammaherpesviruses EBV and herpesvirus saimiri. In common with these viruses, dinucleotide frequency analysis of MHV-68 coding sequences reveals a marked reduction in CpG dinucleotide frequency thus implicating a dividing cell population as the site of latency

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1990-06-01
2024-03-28
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References

  1. Baer R., Bankier A. T., Biggin M. D., Deininger P. L., Farrell P. J., Gibson T. J., Hatfull G., Hudson G. S., Satchwell S. C., Seguin C., Tuffnell P. S., Barrell B. G. 1984; DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature; London: 310207–211
    [Google Scholar]
  2. Bankier A. T., Barrell B. G. 1983; Shotgun DNA sequencing. In Techniques in the Life Sciences B5 pp. 1–33 Flavell R. A. Edited by Dublin: Elsevier;
    [Google Scholar]
  3. Bankier A. T., Dietrich W., Baer R., Barrell B. G., Colbere-Garapin F., Fleckenstein B., Bodemer W. 1985; Terminal repetitive sequences in herpesvirus saimiri virion DNA. Journal of Virology 55:133–139
    [Google Scholar]
  4. Biggin M. D., Gibson T., Hong G. F. 1983; Buffer-gradient gels and 35S label as an aid to rapid DNA sequence determination. Proceedings of the National Academy of Sciences, U.S.A 80:3963–3965
    [Google Scholar]
  5. Bird A. P. 1980; DNA methylation and the frequency of GpG in animal DNA. Nucleic Acids Research 8:1499–1504
    [Google Scholar]
  6. Blaskovic D., Stancekova M., Svobodova J., Kresakova J. 1980; Isolation of five strains of herpesvirus from two species of free living small rodents. Acta virologica 24:468
    [Google Scholar]
  7. Buckmaster A. E., Scott S. D., Sanderson M. J., Boursnell M. E. G., Ross N. L. J., Binns M. M. 1988; Gene sequence and mapping data from Marek’s disease virus and herpesvirus of turkeys: implications for herpesvirus classification. Journal of General Virology 69:2033–2042
    [Google Scholar]
  8. Cameron K. R., Stamminger T., Craxton M., Bodemer W., Honess R. W., Fleckenstein B. 1987; The 160000 Mr virion protein encoded at the right end of the herpesvirussaimiri genome is homologous to the 140000 Mr membrane antigen encoded at the left end of the Epstein-Barr virus genome. Journal of Virology 61:2063–2070
    [Google Scholar]
  9. Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A.III Kouzarides T., Martignetti J. A., Satchwell S. C., Tomlinson P., Weston K. M., Barrell B. G. 1989a; An analysis of the protein coding content of the sequence of human cytomegalovirus strain AD169. Current Topics in Microbiology and Immunology in press
    [Google Scholar]
  10. Chee M. S., Rudolph S.-A., Plachter B., Barrell B., John G. 1989b; Identification of the major capsid protein gene of human cytomegalovirus. Journal of Virology 63:1345–1353
    [Google Scholar]
  11. Ciampor F., Stancekova M., Blaskovic D. 1981; Electron microscopy of rabbit embryo fibroblasts infected with herpesvirus isolates from Clethrionomys glareolus and Apodemus flavicollis. Acta virologica 25:101–107
    [Google Scholar]
  12. Davison A. J., Mcgeoch D. J. 1986; Evolutionary comparisons of the S segments in the genomes of herpes simplex virus type 1 and varicella-zoster virus. Journal of General Virology 67:597–611
    [Google Scholar]
  13. Davison A. J., Scott J. E. 1986; The complete DNA sequence of varicella-zoster virus. Journal of General Virology 67:1759–1816
    [Google Scholar]
  14. Davison A. J., Taylor P. 1987; Genetic relations between varicella-zoster virus and Epstein-Barr virus. Journal of General Virology 68:1067–1079
    [Google Scholar]
  15. Davison A. J., Wilkie N. M. 1983; Location and orientation of homologous sequences in the genomes of five herpesviruses. Journal of General Virology 64:1927–1942
    [Google Scholar]
  16. Dayhoff M. O., Baker W. C., Hunter L. T. 1983; Establishing homologies in protein sequences. Methods in Enzymology 91:524–545
    [Google Scholar]
  17. Efstathiou S., Gompels U. A., Craxton M. A., Honess R. W., Ward K. N. 1988; DNA homology between a novel human herpesvirus (HHV-6) and human cytomegalovirus. Lancet i:63–64
    [Google Scholar]
  18. Efstathiou S., Ho Y. M., Minson A. C. 1990; Cloning and molecular characterization of the murine herpesvirus 68 genome. Journal of General Virology 71:1355–1364
    [Google Scholar]
  19. Fleckenstein B., Desrosiers R. C. 1982; Herpesvirussaimiri and herpesvirusateles. In The Herpesviruses 1 pp. 253–332 Roizman B. Edited by New York & London: Plenum Press;
    [Google Scholar]
  20. Gompels U. A., Craxton M. A., Honess R. W. 1988; Conservation of gene organization in the lymphotropic herpesviruses, herpesvirussaimiri and Epstein-Barr virus. Journal of Virology 62:757–767
    [Google Scholar]
  21. Hirai K., Ikuta K., Kitamoto N., Kato S. 1981; Latency of herpesvirus of turkey and Marek’s disease virus genomes in a chicken T-lymphoblastoid cell line. Journal of General Virology 53:133–143
    [Google Scholar]
  22. Honess R. W. 1984; Herpes simplex and ‘the herpes complex’: diverse observations and a unifying hypothesis. Journal of General Virology 65:2077–2107
    [Google Scholar]
  23. Honess R. W., Watson D. H. 1977; Unity and diversity in the herpesviruses. Journal of General Virology 37:15–37
    [Google Scholar]
  24. Honess R. W., Gompels U. A., Barrell B. G., Craxton M., Cameron K. R., Staden R., Chang Y.-N., Hayward G. S. 1989a; Deviations from expected frequencies of CpGdinucleotides in herpesvirus DNAs may be diagnostic of differences in the states of their latent genomes. Journal of General Virology 70:837–855
    [Google Scholar]
  25. Honess R. W., Craxton M. A., Williams L., Gompels U. A. 19896b; A comparative analysis of the sequence of the thymidine kinase gene of a gammaherpesvirus, herpesvirus saimiri. Journal of General Virology 70:3003–3013
    [Google Scholar]
  26. Kouzarides T., Bankier A. T., Satchwell S. C., Weston K., Tomlinson P., Barrell B. G. 1987; Large-scale rearrangements of homologous regions in the genomes of HCMV and EBV. Virology 157:397–413
    [Google Scholar]
  27. Lawrence G., Chee M. S., Craxton M. A., Gompels U. A., Honess R. W., Barrell B. G. 1989; Human herpesvirus-6 is closely related to human cytomegalovirus. Journal of Virology 64:287–299
    [Google Scholar]
  28. Lipman D. J., Pearson W. R. 1985; Rapid and sensitive protein similarity searches. Science 227:1435–1441
    [Google Scholar]
  29. Mcgeoch D. J., Dolan A., Donald S., Rixon F. J. 1985; Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. Journal of Molecular Biology 181:1–13
    [Google Scholar]
  30. Mcgeoch D. J., Dalrymple M. A., Davison A. J., Dolan A., Frame M. C., Mcnab D., Perry L. J., Scott J. E., Taylor P. 1988; The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. Journal of General Virology 69:1531–1574
    [Google Scholar]
  31. Minson A. C. 1989; Herpesviridae. In Andrewe’s Viruses of Vertebrates pp. 293–332 Porterfield J. S. Edited by London: Ballière Tindall;
    [Google Scholar]
  32. Mistrikova J., Blaskovic D. 1985; Ecology of the murine alpha herpesvirus and its isolation from lungs of rodents in cell culture. Acta virologica 29:312–317
    [Google Scholar]
  33. Payne L. N. 1982; Biology of Marek’s disease virus and the herpesvirus of turkeys. In The Herpesviruses 1 pp. 343–431 Roizman B. Edited by New York: Plenum Press;
    [Google Scholar]
  34. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparisons. Proceedings of the National Academy of Sciences, U.S.A 85:2444–2448
    [Google Scholar]
  35. Roizman B. 1982; The family herpesviridae: general description taxonomy and classification. In The Herpesviruses 1 pp. 1–23 Roizman B. Edited by New York: Plenum Press;
    [Google Scholar]
  36. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, U.S.A 74:5463–5467
    [Google Scholar]
  37. Staden R. 1986; The current status and portability of our sequence handling software. Nucleic Acids Research 14:217–231
    [Google Scholar]
  38. Stamminger T., Honess R. W., Young D. F., Bodemer W., Blair E. D., Fleckenstein B. 1987; Organization of terminal reiterations in the virion DNA of herpesvirus saimiri. Journal of General Virology 68:1049–1066
    [Google Scholar]
  39. Svobodova J., Blaskovic D., Mistrikova I. 1982a; Growth characteristics of herpesviruses isolated from free living small rodents. Acta virologica 26:256–263
    [Google Scholar]
  40. Svobodova J., Stancekova M., Blaskovic D., Mistrikova J., Lesso J., Russ G., Masarova P. 1982b; Antigenic relatedness of alphaherpesviruses isolated from free-living rodents. Acta virologica 26:428–443
    [Google Scholar]
  41. Tautz D., Renz M. 1983; An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels. Analytical Biochemistry 132:14–19
    [Google Scholar]
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