1887

Journal of General Virology header logo

Volume 71, Issue 6

Conformation of the VP2 protein of bluetongue virus (BTV) determines the involvement in virus neutralization of highly conserved epitopes within the BTV serogroup Free

Abstract

Seven neutralizing monoclonal antibodies (N-MAbs) were generated to an Australian isolate of bluetongue virus serotype 1 [BTV-1 (Aust)]. At least five of the N- MAbs were specific for epitopes on the outer coat protein VP2 and one was capable of binding SDS- treated protein in a Western blot. Six of the N-MAb panel bound and four of these neutralized BTV-1 (South Africa). None of the N-MAbs neutralized other Australian or South African BTV serotypes. However four of the N-MAb panel bound to a majority and two others bound with varying efficiency, to a more restricted but significant number of heterologous serotypes. Thus epitopes involved in the definition of one BTV serotype may be preserved on other serotypes but not be involved in their neutralization. To investigate the association between these epitopes and factors governing their expression, pools of neutralization- escape variants of BTV-1 (Aust), selected using each of six N-MAbs, were tested in virus neutralization and ELISA binding assays against the N-MAb panel. Each N-MAb displayed a unique reaction pattern with all 25 variants tested. All variants except one showed resistance to neutralization and/or reduced binding with at least three heterologous N-MAbs indicating the N- MAb-defined epitopes were mutually interactive. All 25 variants demonstrated increased resistance to neutralization by a bovine antiserum to BTV-1 (Aust). In total, the results from the variants revealed that the N-MAbs define seven distinct, interdependent neutralization epitopes which form at least part of a major neutralization domain on BTV. A majority of variants bound at least four and up to six N-MAbs, yet still resisted neutralization by them. This observation and the reaction of N-MAbs with BTV-1 (South Africa) and heterologous serotypes suggested that the conformation of the VP2 protein determines whether epitopes, conserved within the BTV serogroup, are involved in neutralization of individual serotypes of the virus.

  • Received:
  • Accepted:
  • Published Online:
© Society for General Microbiology 1990
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-6-1325
1990-06-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/6/JV0710061325.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-6-1325&mimeType=html&fmt=ahah

References

  1. Appleton J. A., Letchworth G. J. 1983; Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124:286–299
     [Google Scholar]
  2. Blondel B., Crainic R., Fichot O., Dufraisse G., Candrea A., Diamond D., Girard M., Horaud F. 1986; Mutations conferring resistance to neutralization with monoclonal antibodies in type 1 poliovirus can be located outside or inside the antibodybinding site. Journal of Virology 57:81–90
     [Google Scholar]
  3. Breschkin A. M., Ahern J., White D. O. 1981; Antigenic determinants of influenza virus hemagglutinin. VIII. Topography of the antigenic regions of influenza virus hemagglutinin determined by competitive radioimmunoassay with monoclonal antibodies. Virology 113:130–140
     [Google Scholar]
  4. Cowley J. A., Gorman B. M. 1989; Cross-neutralization of genetic reassortants of bluetongue virus serotypes 20 and 21. Veterinary Microbiology 19:37–51
     [Google Scholar]
  5. Della-Porta A. J., Herniman K. A. J., Sellers R. F. 1981a; A serological comparison of the Australian isolate of bluetongue virus type 20 (CSIR019) with bluetongue group viruses. Veterinary Microbiology 6:9–21
     [Google Scholar]
  6. Della-Porta A. J., Mcphee D. A., Wark M. C., St George T. D., Cybinski D. H. 1981b; Serological studies of two additional Australian bluetongue virus isolates CSIR0154 and CSIR0156. Veterinary Microbiology 6:233–245
     [Google Scholar]
  7. Eaton B. T., Hyatt A. D., White J. R. 1987; Association of bluetongue virus with the cytoskeleton. Virology 157:107–116
     [Google Scholar]
  8. Eaton B. T., Hyatt A. D., White J. R. 1988; Localization of the nonstructural protein NS1 in bluetongue virus-infected cells and its presence in virus particles. Virology 163:527–537
     [Google Scholar]
  9. Garcia-Barreno B., Sanz A., Nogal M. L., Vinuela E., Enjuanes L. 1986; Monoclonal antibodies to African swine fever virus: antigen differences among field virus isolates and viruses passaged in cell culture. Journal of Virology 58:385–392
     [Google Scholar]
  10. Gerhard W., Yewdell J., Frankel M. E., Webster R. 1981; Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies. Nature; London: 290713–717
     [Google Scholar]
  11. Ghiasi H., Fukusho A., Eshita Y., Roy P. 1987; Identification and characterization of conserved and variable regions in the neutralization VP2 gene of bluetongue virus. Virology 160:100–109
     [Google Scholar]
  12. Gould A. R., Hyatt A. D., Eaton B. T. 1988; Morphogenesis of a bluetongue virus variant with an amino acid alteration at a neutralization site in the outercoat protein, VP2. Virology 165:23–32
     [Google Scholar]
  13. Huismans H., Erasmus B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Science 48:51–58
     [Google Scholar]
  14. Huismans H., van der Walt N. T., Cloete M., Erasmus B. J. 1987; Isolation of a capsid protein of bluetongue virus that induces a protective immune response in sheep. Virology 157:172–179
     [Google Scholar]
  15. Icenogle J., Shiwen H., Duke G., Gilbert S., Rueckert R., Anderegg J. 1983; Neutralization of poliovirus by a monoclonal antibody: kinetics and stoichiometry. Virology 127:412–425
     [Google Scholar]
  16. Letchworth G. J., Appleton J. A. 1983; Heterogeneity of neutralization-related epitopes within a bluetongue virus serotype. Virology 124:300–307
     [Google Scholar]
  17. Lunt R. A., White J. R., Della-Porta A. J. 1988a; Studies with enzyme-linked immunosorbent assays for the serodiagnosis of bluetongue and epizootic haemorrhagic disease of deer. Veterinary Microbiology 16:323–338
     [Google Scholar]
  18. Lunt R. A., White J. R., Blacksell S. D. 1988b; Evaluation of a monoclonal antibody blocking ELISA for the detection of group-specific antibodies to bluetongue virus in experimental antisera and field sera. Journal of General Virology 69:2729–2740
     [Google Scholar]
  19. Mccahon D., Crowther J. R., Belsham G. J., Kitson J. D. A., Duchesne M., Have P., Meloen R. H., Morgan D. O., de Simone F. 1989; Evidence for at least four antigenic sites on type O foot-and-mouth disease virus involved in neutralization; identification by single and multiple site monoclonal antibody-resistant mutants. Journal of General Virology 70:639–645
     [Google Scholar]
  20. Mertens P. R. G., Pedley S., Cowley J., Burroughs J. N., Coreyn A. H., Jeggo M. H., Jennings D. M., Gorman B. M. 1989; Analysis of the roles of bluetongue virus outer capsid proteins VP2 and VP5 in determination of virus serotype. Virology 170:561–565
     [Google Scholar]
  21. Ristow S., Leendersten L., Gorham J., Yilma T. 1988; Identification of a neutralizing epitope shared by bluetongue virus serotypes 2 and 13. Journal of Virology 62:2502–2504
     [Google Scholar]
  22. White J. R., Breschkin A. M., Della-Porta A. J. 1985; Immunochemical analyses of Australian bluetongue virus serotypes using monoclonal antibodies. In Bluetongue and Related Orbiviruses pp. 397–405 Barber T. L., Jochim M. M. Edited by New York: Alan R. Liss;
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-6-1325
Loading
Conformation of the VP2 protein of bluetongue virus (BTV) determines the involvement in virus neutralization of highly conserved epitopes within the BTV serogroup
J. Gen. Virol. 71, 1325 (1990); https://doi.org/10.1099/0022-1317-71-6-1325
/content/journal/jgv/10.1099/0022-1317-71-6-1325
/content/journal/jgv/10.1099/0022-1317-71-6-1325
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error