1887

Abstract

Plasmid vectors were constructed to facilitate the insertion and expression of a foreign gene in the haemagglutinin (HA) gene locus of vaccinia virus. Five unique cloning sites adjacent to the P7.5 promoter of vaccinia virus permit the rapid insertion of a foreign sequence coding for a protein into these plasmids. This vector system provides a simple procedure to select recombinant viruses because they can be readily identified on the basis of their HA-defective phenotype. Recombinant vaccinia viruses expressing influenza virus HA were constructed to characterize the possible use of this system. The recombinant viruses did express the influenza HA through the authentic pathway of biosynthesis. In addition to having immunological characteristics similar to the authentic influenza HA, the expressed HA was found to possess haemagglutinating, haemadsorption and acid-inducible fusion activities. These findings demonstrate the usefulness of this eukaryotic vector system.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-6-1293
1990-06-01
2022-08-15
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/6/JV0710061293.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-6-1293&mimeType=html&fmt=ahah

References

  1. Boyle D. B., Coupar B. E. H., Both G. W. 1985; Multiplecloning-site plasmids for the rapid construction of recombinant poxviruses. Gene 35:169–177
    [Google Scholar]
  2. Chamberlain J. P. 1979; Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Analytical Biochemistry 98:132–135
    [Google Scholar]
  3. Chambers T. M., Kawaoka Y., Webster R. G. 1988; Protection of chickens from lethal influenza infection by vaccinia-expressed hemagglutinin. Virology 167:414–421
    [Google Scholar]
  4. Copeland C. S., Doms R. W., Bolzau E. M., Webster R. G., Helenius A. 1986; Assembly of influenza hemagglutinintrimers and its role in intracellular transport. Journal of Cell Biology 103:1179–1191
    [Google Scholar]
  5. Copeland C. S., Zimmer K.-P., Wagner K. R., Healey G. A., Mellman I., Helenius A. 1988; Folding, trimerization, and transport are sequential events in the biogenesis of influenza virus hemagglutinin. Cell 53:197–209
    [Google Scholar]
  6. Davis A. R., Nayak D. P., Ueda M., Hiti A. L., Dowbenko D., Kleid D. G. 1981; Expression of antigenic determinants of the hemagglutinin gene of a human influenza virus in Escherichia coli. Proceedings of the National Academy of Sciences, U.S.A 78:5376–5380
    [Google Scholar]
  7. De B. K., Shaw M. W., Rota P. A., Harmon M. W., Esposito J. J., Rott R., Cox N. J., Kendal A. P. 1988; Protection against virulent H5 avian influenza virus infection in chickens by an inactivated vaccine produced with recombinant vaccinia virus. Vaccine 6:257–261
    [Google Scholar]
  8. Doms R. W., Helenius A. 1986; Quaternary structure of influenza virus hemagglutinin after acid treatment. Journal of Virology 60:833–839
    [Google Scholar]
  9. Dunphy W. G., Rothman J. S. 1985; Compartmental organization of the Golgi stack. Cell 42:13–21
    [Google Scholar]
  10. Emtage J. S., Tacon W. C. A., Catlin G. H., Jenkins B., Porter A. G., Carey N. H. 1980; Influenza antigenic determinants are expressed from haemagglutinin genes cloned in Escherichia coli. Nature; London: 283171–174
    [Google Scholar]
  11. Fathi Z., Sridhar P., Pacha R. F., Condit R. C. 1986; Efficient targeted insertion of an unselected marker into the vaccinia virus genome. Virology 155:97–105
    [Google Scholar]
  12. Funahashi S., Sato T., Shida H. 1988; Cloning and characterization of the gene encoding the major protein of the A-type inclusion body of cowpox virus. Journal of General Virology 69:35–47
    [Google Scholar]
  13. Gething M.-J., Sambrook J. 1981; Cell-surface expression of influenza haemagglutinin from a cloned DNA copy of the RNA gene. Nature; London: 293620–625
    [Google Scholar]
  14. Gething M.-J., Mccammon K., Sambrook J. 1986; Expression of wild-type and mutant forms of influenza hemagglutinin: the role of folding in intracellular transport. Cell 46:939–950
    [Google Scholar]
  15. Heiland I., Gething M.-J. 1981; Cloned copy of the haemagglutinin gene codes for human influenza antigenic determinants in E. coli. Nature; London: 292851–852
    [Google Scholar]
  16. Huang R. T. C., Rott R., Klenk H.-D. 1981; Influenza viruses cause hemolysis and fusion of cells. Virology 110:243–247
    [Google Scholar]
  17. Ichihashi Y., Dales S. 1971; Biogenesis of poxviruses: interrelationship between hemagglutinin production and polykaryocytosis. Virology 46:533–543
    [Google Scholar]
  18. Jabbar M. A., Sivasubramanian N., Nayak D. P. 1985; Influenza viral (A/WSN/33) hemagglutinin is expressed and glycosylated in the yeast Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences, U.S.A 82:2019–2023
    [Google Scholar]
  19. Joklik W. K. 1962; The purification of four strains of poxvirus. Virology 18:9–18
    [Google Scholar]
  20. Kuroda K., Hauser C., Rott R., Klenk H.-D., Doerfler W. 1986; Expression of the influenza virus haemagglutinin in insect cells by a baculovirus vector. EMBO Journal 5:1359–1365
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  22. Mackett M., Smith G. L. 1986; Vaccinia virus expression vectors. Journal of General Virology 67:2067–2082
    [Google Scholar]
  23. Mackett M., Smith G. L., Moss B. 1985; The construction and characterization of vaccinia virus recombinants expressing foreign genes. In DNA Cloning 2 pp. 191–211 Glover D. M. Edited by Oxford: IRL Pess;
    [Google Scholar]
  24. Maeda T., Kawasaki K., Ohnishi S. 1981; Interaction of influenza virus hemagglutinin with target membrane lipids is a key step in virus-induced hemolysis and fusion at pH 5.2. Proceedings of the National Academy of Sciences, U.S.A 78:4133–4137
    [Google Scholar]
  25. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Matlin K., Reggio H., Helenius A., Simons K. 1981; Infectious entry mechanism of influenza virus in a canine kidney cell line. Journal of Cell Biology 91:601–613
    [Google Scholar]
  27. Moss B., Flexner C. 1987; Vaccinia virus expression vectors. Annual Review of Immunology 5:305–324
    [Google Scholar]
  28. Panicali D., Davis S. W., Weinberg R. L., Paoletti E. 1983; Construction of live vaccines by using genetically engineered poxviruses: biological activity of recombinant vaccinia virus expressing influenza virus hemagglutinin. Proceedings of the National Academy of Sciences, U.S.A 80:5364–5368
    [Google Scholar]
  29. Perkus M. E., Piccini A., Lipinskas B. R., Paoletti E. 1985; Recombinant vaccinia virus: immunization against multiple pathogens. Science 229:981–984
    [Google Scholar]
  30. Rota P. A., Shaw M. W., Kendal A. P. 1987; Comparison of the immune response to variant influenza type B hemagglutinins expressed in vaccinia virus. Virology 161:269–275
    [Google Scholar]
  31. Shida H. 1986; Nucleotide sequence of the vaccinia virus hemagglutinin gene. Virology 150:451–462
    [Google Scholar]
  32. Shida H., Matsumoto S. 1983; Analysis of the hemagglutinin glycoprotein from mutants of vaccinia virus that accumulates on the nuclear envelope. Cell 33:423–433
    [Google Scholar]
  33. Shida H., Tochikura T., Sato T., Konno T., Hirayoshi K., Seki M., Ito Y., Hatanaka M., Hinuma Y., Sugimoto M., Takahashi-Nishimaki F., Maruyama T., Miki K., Suzuki K., Morita M., Sashiyama H., Hayami M. 1987; Effect of the recombinant vaccinia viruses that express HTLV-I envelope gene on HTLV-I infection. EMBO Journal 6:3379–3384
    [Google Scholar]
  34. Shida H., Hinuma Y., Hatanaka M., Morita M., Kidokoro M., Suzuki K., Maruyama T., Takahashi-Nishimaki F., Sugimoto M., Kitamura R., Miyazawa T., Hayami M. 1988; Effects and virulences of recombinant vaccinia viruses derived from attenuated strains that express the human T-cell leukemia virus type I envelope gene. Journal of Virology 62:4474–4480
    [Google Scholar]
  35. Smith G. L., Murphy B. R., Moss B. 1983; Construction and characterization of an infectious vaccinia virus recombinant that expresses the influenza hemagglutinin gene and induces resistance to influenza virus infection in hamsters. Proceedings of the National Academy of Sciences, U.S.A 80:7155–7159
    [Google Scholar]
  36. Smith G. L., Levin J. Z., Palese P., Moss B. 1987; Synthesis and cellular location of the ten influenza polypeptides individually expressed by recombinant vaccinia viruses. Virology 160:336–345
    [Google Scholar]
  37. Stephens E. B., Compans R. W., Earl P., Moss B. 1986; Surface expression of viral glycoproteins is polarized in epithelial cells infected with recombinant vaccinia viral vectors. EMBO Journal 5:237–245
    [Google Scholar]
  38. Venkatesan S., Baroudy B. M., Moss B. 1981; Distinctive nucleotide sequence adjacent to multiple initiation and termination sites of an early vaccinia virus gene. Cell 35:805–813
    [Google Scholar]
  39. White J., Matlin K., Helenius A. 1981; Cell fusion by Semliki Forest, influenza, and vesicular stomatitis viruses. Journal of Cell Biology 89:674–679
    [Google Scholar]
  40. Yewdell J. W., Bennink J. R., Smith G. L., Moss B. 1985; Influenza A virus nucleoprotein is a major target antigen for crossreactive anti-influenza A virus cytotoxic T lymphocytes. Proceedings of the National Academy of Sciences, U.S.A 82:1785–1789
    [Google Scholar]
  41. Yewdell J. W., Yellen A., Bachi T. 1988; Monoclonal antibodies localize events in the folding, assembly, and intracellular transport of the influenza virus hemagglutinin glycoprotein. Cell 52:843–852
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-6-1293
Loading
/content/journal/jgv/10.1099/0022-1317-71-6-1293
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error