1887

Abstract

A panel of monoclonal antibodies which react with empty non-infectious type 3 poliovirus particles (C antigen) but not infectious virus (D antigen) were characterized for their reactivity with C antigen particles derived from neutralization-resistant virus strains which had single amino acid substitutions at each of the antigenic sites. Antibodies were identified which failed to bind to variant viruses with modifications at each of antigenic sites 2b, 3b and 4 indicating that the same amino acid sequences involved in the neutralization of infectious virus are also present on the surface of noninfectious particles but in different configurations.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-6-1271
1990-06-01
2022-10-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/6/JV0710061271.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-6-1271&mimeType=html&fmt=ahah

References

  1. Brown E., Sheppard R. C., Williams B. J. 1983; Peptide synthesis. Part 5. Solid-phase synthesis of (15-leucine) little gastrin. Journal of the Chemical Society Perkins Transactions 1:1161–1167
    [Google Scholar]
  2. Ferguson M., Minor P. D., Magrath D. I., Qui Y.-H., Spitz M., Schild G. C. 1984; Neutralization epitopes on poliovirus type 3 particles: an analysis using monoclonal antibodies. Journal of General Virology 65:197–201
    [Google Scholar]
  3. Ferguson M., Evans D. M. A., Magrath D. I., Minor P. D., Almond J. W., Schild G. C. 1985; Induction by synthetic peptides of broadly reactive, type-specific neutralizing antibody to poliovirus type 3. Virology 143:505–515
    [Google Scholar]
  4. Fernandez-Tomas C. B., Baltimore D. 1973; Morphogenesis of poliovirus. II. Demonstration of a new intermediate, the provirion. Journal of Virology 12:1122–1130
    [Google Scholar]
  5. Filman D. J., Syed R., Chow M., Macadam A. J., Minor P. D., Hogle J. M. 1989; Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO Journal 8:1567–1579
    [Google Scholar]
  6. Hogle J. M., Chow M., Filman D. J. 1985; The three dimensional structure of poliovirus at 2·9 Å resolution. Science 229:1358–1365
    [Google Scholar]
  7. Mayer M. M., Rapp H. J., Roizman B., Klein S. W., Cowan K. M., Lukens D., Schwerdt C. E., Schaffer F. L., Charney J. 1957; The purification of poliomyelitis virus as studied by complement fixation. Journal of Immunology 78:435–455
    [Google Scholar]
  8. Minor P. D., Schild G. C., Wood J. M., Dandawate C. N. 1980; The preparation of specific immune sera against type 3 poliovirus D-antigen and C-antigen and the demonstration of two C-antigenic components in vaccine strain populations. Journal of General Virology 51:147–156
    [Google Scholar]
  9. Minor P. D., Schild G. C., Bootman J., Evans D. M. A., Ferguson M., Reeve P., Spitz M., Stanway G., Cann A. J., Hauptmann R., Clarke L. D., Mountford R. C., Almond J. W. 1983; Location and primary structure of a major antigenic site for poliovirus neutralization. Nature; London: 301674–679
    [Google Scholar]
  10. Minor P. D., Evans D. M. A., Ferguson M., Schild G. C., Westrop G., Almond J. W. 1985; Principal and subsidiary antigenic sites of VP1 involved in the neutralization of poliovirus type 3. Journal of General Virology 66:1159–1165
    [Google Scholar]
  11. Minor P. D., Ferguson M., Evans D. M. A., Almond J. W., Icenogle J. P. 1986; Antigenic structure of polioviruses of serotypes 1, 2 and 3. Journal of General Virology 67:1283–1291
    [Google Scholar]
  12. Minor P. D., Ferguson M., Phillips A., Magrath D. I., Huovilainen A., Hovi T. 1987; Conservation in vivo of protease cleavage sites in antigenic sites of poliovirus. Journal of General Virology 68:1857–1865
    [Google Scholar]
  13. Page G. S., Mosser A. G., Hogle J. M., Filman D. J., Rueckert R. R., Chow M. 1988; Three-dimensional structure of poliovirus serotype 1 neutralizing determinants. Journal of Virology 62:1781–1794
    [Google Scholar]
  14. Schild G. C., Wood J. M., Minor P. D., Dandawate C. N., Magrath D. I. 1980; Immunoassay of poliovirus antigens by single-radial-diffusion: development and characteristics of sensitive autoradiographic zone size enhancement (ZE) technique. Journal of General Virology 51:157–170
    [Google Scholar]
  15. Wiegers K. J., Uhlig H., Dernick R. 1989; N-AgIB of poliovirus type 1: a discontinuous epitope formed by two loops of VP1 comprising residues 96-104 and 141-152. Virology 170:583–586
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-71-6-1271
Loading
/content/journal/jgv/10.1099/0022-1317-71-6-1271
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error