Evidence for the Role of Subgenomic RNAs in the Production of Potato Virus S coat Protein During Translation Free

Abstract

Purified virus particles of potato virus S (PVS) when disrupted yielded only one readily resolved band of 7·5 kb in ethidium bromide-stained agarose gels. However Northern hybridization of viral RNA, probed with a clone specific to the viral coat protein gene, revealed a region of subgenomic RNAs of approximately 1·3 kb. Sucrose gradient fractionation of subgenomic RNA revealed that it coded for viral coat protein when translated in rabbit reticulocyte lysate. Virus particle lengths suggest that this subgenomic RNA may be encapsidated in 100 to 220 nm particles.

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1990-05-01
2024-03-29
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References

  1. Ahlquist P., Dasgupta R., Kaesberg P. 1984; Nucleotide sequence of the brome mosaic virus genome and its implications for virus replication. Journal of Molecular Biology 172:369–383
    [Google Scholar]
  2. Forster R. L. S., Guilford P. J., Faulds D. V. 1987; Characterization of the coat protein subgenomic RNA of white clover mosaic virus. Journal of General Virology 68:181–190
    [Google Scholar]
  3. Foster G. D., Mills P. R. 1990; Detection of strains of potato virus S by nucleic acid spot hybridization (NASH). Potato Research in press
    [Google Scholar]
  4. Goelet P., Lomonossoff G. P., Butler P. J. G., Akam M. E., Gait M. J., Karn J. 1982; Nucleotide sequence of tobacco mosaic virus RNA. Proceedings of the National Academy of Sciences U.S.A: 795818–5822
    [Google Scholar]
  5. Harbison S. A., Wilson M. A., Davies J. W. 1984; An encapsidated, subgenomic messenger RNA encodes the coat protein of carnation mottle virus. Bioscience Reports 4:949–956
    [Google Scholar]
  6. Koenig R. 1982; Carlavirus group. CMI/AAB Descriptions of Plant Viruses259
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227:680–685
    [Google Scholar]
  8. Mackenzie D. J., Tremaine J. H., Stace-Smith R. 1989; Organization and interviral homologies of the 3′-terminal portion of potato virus S RNA. Journal of General Virology 70:1053–1063
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Mayo M. A., Reddy D. V. R. 1985; Translation products of RNA from Indian peanut clump virus. Journal of General Virology 66:1347–1351
    [Google Scholar]
  11. Meinkoth J., Wahl G. 1984; Hybridization of nucleic acids immobilized on solid supports. Analytical Biochemistry 138:267–284
    [Google Scholar]
  12. Perbal B. V. 1984 A Practical Guide to Molecular Cloning New York; Wiley-lnterscience:
    [Google Scholar]
  13. Rose D. G. 1983; Some properties of an unusual isolate of potato virus S. Potato Research 26:49–62
    [Google Scholar]
  14. Rupasov V. V., Morozov S. YU., Kanyuka K. V., Zavriev S. K. 1989; Partial nucleotide sequence of potato virus M RNA shows similarities to potexviruses in gene arrangement and the encoded amino acid sequences. Journal of General Virology 70:1861–1869
    [Google Scholar]
  15. Shields S. A., Wilson T. M. A. 1987; Cell-free translation of turnip mosaic virus RNA. Journal of General Virology 68:169–180
    [Google Scholar]
  16. Short M. N., Davies J. W. 1983; Narcissus mosaic virus: a potexvirus with an encapsidated subgenomic messenger RNA for coat protein. Bioscience Reports 3:837–846
    [Google Scholar]
  17. Szybiak U., Legocki A. B. 1981; Translation in vitro of ribonucleic acids from potato virus X, potato virus M and white clover mosaic virus. Acta biochimica polonica 28:99–104
    [Google Scholar]
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