1887

Abstract

Ten monoclonal antibodies have been raised against lysates of cells infected with cytopathic border disease virus (BDV). These antibodies all recognize non- cytopathic BDV and react with a number of different strains of bovine viral diarrhoea virus (BVDV). Studies with radiolabelled cell lysates show that all the antibodies precipitate two polypeptides of apparent 80000 and 130000 from cells infected with cytopathic virus and a single polypeptide of apparent 120000 from cells infected with non-cytopathic virus. Two of the monoclonal antibodies react on immunoblots and show the same pattern of reactivity indicating that these three polypeptides are antigenically related.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-71-5-1227
1990-05-01
2024-10-13
Loading full text...

Full text loading...

/deliver/fulltext/jgv/71/5/JV0710051227.html?itemId=/content/journal/jgv/10.1099/0022-1317-71-5-1227&mimeType=html&fmt=ahah

References

  1. Barlow R. M. 1972; Experiments in border disease. IV. Pathological changes in ewes. Journal of Comparative Pathology 82:151–157
    [Google Scholar]
  2. Bolin S., Moennig V., Kelso Gourley N. E., Ridpath J. 1988; Monoclonal antibodies with neutralising activity segregate isolates of bovine viral diarrhea virus into groups. Archives of Virology 99:117–123
    [Google Scholar]
  3. Brodeur B. R., Tsang P., Larose Y. 1984; Parameters affecting ascites tumour formation. Journal of Immunological Methods 71:265–272
    [Google Scholar]
  4. Cay B., Chappuis G., Coulibaly C., Dinter Z., Edwards S., Greiser-Wilke I., Gunn M., Have P., Hess G., Juntti N., Liess B., Mateo A., Mchugh P., Moennig V., Nettleton P., Wensvoort G. 1989; Comparative analysis of monoclonal antibodies against pesti viruses: report of an international workshop. Veterinary Microbiology 20:123–129
    [Google Scholar]
  5. Collett M. S., Larson R., Gold C., Strick D., Anderson D. K., Purchoi A. F. 1988a; , Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology 165:191–199
    [Google Scholar]
  6. Collett M. S., Larson R., Belzer S. K., Retzel E. 1988b; Proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. Virology 165:200–208
    [Google Scholar]
  7. Collett M. S., Moennig V., Horzinek M. C. 1989; Recent advances in pestivirus research. Journal of General Virology 70:253–266
    [Google Scholar]
  8. Donis R. O., Dubovi E. J. 1987a; Characterization of bovine viral diarrhoea-mucosal disease virus-specific proteins in bovine cells. Journal of General Virology 68:1597–1605
    [Google Scholar]
  9. Donis R. O., Dubovi E. J. 1987b; Glycoproteins of bovine viral diarrhoea-mucosal disease virus in infected bovine cells. Journal of General Virology 68:1607–1616
    [Google Scholar]
  10. Donis R. O., Dubovi E. J. 1987c; Differences in virus-induced polypeptides in cells infected by cytopathic and noncytopathic biotypes of bovine virus diarrhea-mucosal disease virus. Virology 158:1597–1605
    [Google Scholar]
  11. Donis R. O., Corapi W., Dubovi E. J. 1988; Neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56K to 58K glycoprotein. Journal of General Virology 69:77–86
    [Google Scholar]
  12. Edwards S., Sands J. J., Harkness J. W. 1988; The application of monoclonal antibody panels to characterise pestivirus isolates from ruminants in Great Britain. Archives of Virology 102:197–206
    [Google Scholar]
  13. Fazekas De St Groth F., Scheidegger D. 1980; Production of monoclonal antibodies: strategy and tactics. Journal of Immuno-logical Methods 35:1–21
    [Google Scholar]
  14. Gardiner A. C., Nettleton P. F., Barlow R. M. 1983; Virology and immunology of a spontaneous and experimental mucosal-like syndrome in sheep recovered from clinical border disease. Journal of Comparative Pathology 93:463–469
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–684
    [Google Scholar]
  16. Nettleton P. F. 1987; Pathogenesis and epidemiology of border disease. Annales de Recherche Veterinaire 18:147–155
    [Google Scholar]
  17. Nettleton P. F., Gilmour J. S., Barlow R. M., Gardiner A. C. 1987; Border disease: the different effects of non-cytopathic and cytopathic virus in pregnant sheep. Abstracts of VII International Congress of Virology Edmonton, Alberta, Canada:August9–14 p 81
    [Google Scholar]
  18. Osburn B. I., Clarke G. L., Stewart W. C., Sawyer M. 1973; Border disease like syndrome in lambs: antibodies to hog cholera and bovine viral diarrhea viruses. Journal of the American Veterinary Medical Association 163:1165–1167
    [Google Scholar]
  19. Peters W., Greiser-Wilke I., Moennig V., Liess B. 1986; Preliminary serological characterisation of viral diarrhoea virus strains using monoclonal antibodies. Veterinary Microbiology 12:195–200
    [Google Scholar]
  20. Pocock D. H., Howard C. J., Clarke M. C., Brownlie J. 1987; Variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus. Archives of Virology 94:43–53
    [Google Scholar]
  21. Purchio A. F., Larson R., Collett M. S. 1984; Characterization of bovine viral diarrhea virus proteins. Journal of Virology 50:666–669
    [Google Scholar]
  22. Renard A., Guiot C., Schmetz D., Dagenais L., Pastoret P. P., Dina D., Martial J. A. 1985; Molecular cloning of bovine viral diarrhoea virus sequences. DNA 4:429–438
    [Google Scholar]
  23. Showalter S. D., Zweig M., Hampar B. 1981; Monoclonal antibodies to herpes simplex virus type 1 proteins including the immediate-early protein 1CP 4. Infection and Immunity 34:684–692
    [Google Scholar]
  24. Vantsis J. T., Barlow R. M., Fraser J., Rennie J. C., Mould D. L. 1976; Experiments in border disease. VIII. Propagation and properties of a cytopathic virus. Journal of Comparative Pathology 86:111–120
    [Google Scholar]
  25. Vantsis J. T., Barlow R. M., Gardiner A. C. 1980; The effects of challenge with homologous and heterologous strains of border disease virus on ewes with previous experience of the disease. Journal of Comparative Pathology 90:39–45
    [Google Scholar]
  26. Wensvoort G., Terpstra C., Boonstra J., Bloemraad M., Vanzaane D. 1986; Production of monoclonal antibodies against swine fever virus and their use in laboratory diagnosis. Veterinary Microbiology 12:101–108
    [Google Scholar]
  27. Westaway E. G., Brinton M. A., Gaidamovich S. YA., Horzinek M. C., Igarashi A., Kääriäinen L., Lvov D. K., Porterfield J. S., Russell P. K., Trent D. W. 1985; Togaviridae. Intervirology 24:125–139
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-71-5-1227
Loading
/content/journal/jgv/10.1099/0022-1317-71-5-1227
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error